Elicitor-induced indole alkaloid biosynthesis in Catharanthus roseus cell cultures is related to Ca2+ influx and the oxidative burst

Zhao, Jian; Hu, Qingyuan; Guo, Yingqing; Zhu, Weiming

doi:10.1016/s0168-9452(01)00422-8

Cited by 88 publications

(34 citation statements)

References 27 publications

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“…(2005) shows that CAT activity was lower in JA treated Morinda elliptica cell cultures after 6 days of elicitation. The suppression in CAT activity have similarly been reported in fungal-elicited Catharanthus roseus cell cultures (Zhao et al, 2001) and water-stressed in strawberry plants (Wang, 1999). In contrast, MJ treatment of strawberry plant increases CAT activity (Wang, 1999).…”

Section: Discussionsupporting

confidence: 57%

“…In our work, induction of peroxidases (POD) in melon cells during the time course of elicitation, may result from enzyme activation and/or the de novo differential expression in POD isoforms, implicating them in stress alleviation, as POD expression is controlled by multiple genes (Gomez-vasquez et al, 2004). On the other hand, Bestwick et al (1998);Martinez et al (1998) and Zhao et al (2001) found that apoplastic peroxidases have been responsible for the oxidative burst in an increasing list of plant species. Cell wall-bound POD enzyme activity in melon tissues treated with JA collaborates in H 2 O 2 production, but it is not the major factor, but implicates it in cells membrane stability disturbance resulted in transient leakage of important compounds into growth media.…”

Section: Discussionmentioning

confidence: 73%

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Jasmonic acid elicits oxidative defense and detoxification systems in Cucumis melo L. cells

Nafie¹,

Hathout²,

Mokadem³

2011

Braz. J. Plant Physiol.

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This study investigated whether a jasmonic acid (JA) elicitation strategy developed in a conventional cell suspension culture could evoke melon resistance mechanisms, including secondary metabolite production. Twenty one day cultured melon cell suspensions grown in MS1 medium were supplemented with JA at the concentrations of 0.5, 5.0 and 10 µmol. Melon cultures were sampled 24, 48 and 72 h post elicitation to evaluate different defense related factors such as antioxidant enzymes, ascorbate metabolism and phenolic compounds. Results suggest that melon cells respond to JA reprogramming the primary and secondary metabolism which will result in melon plantlets with enhanced resistance against diverse stress conditions through the production of specific bioactive molecules. Jasmonic acid elicited melon cells exhibited enhanced oxidative enzymes activities and ascorbic acid, coumarin and p-coumaric amounts were found without growth retardation. Induced intracellular JA functions as a signal transducer acting upstream to H 2 O 2 , which is a secondary messenger triggering jasmonic signaling cascades by activating certain late genes that regulate the activity of catalase, peroxidase and de novo synthesis of five isozymes, ascorbic peroxidase detoxifying enzymes concomitant with ascorbate compound. Secondary metabolite production in melon cells seems to be activated upon JA exposure suggesting that this cell culture could be used as a source for rapid and increased production of coumarin, p-coumaric, ascorbic acid and likely other specific phenylpropanoids. These data provides further evidences for a role of jasmonic acid in the intracellular signal cascade that results in the accumulation of secondary compounds and ultimately induced melon resistance. This approach could assist further in understanding the metabolic mechanisms operating in melon cells under stress, and thus how to control them.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 73%

Jasmonic acid elicits oxidative defense and detoxification systems in Cucumis melo L. cells

Nafie¹,

Hathout²,

Mokadem³

2011

Braz. J. Plant Physiol.

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“…In contrast, the CAT activity was suppressed in MJ treatment while the GPx level was increased, which could suggest that the CAT functions in H 2 O 2 removal were compensated by the GPx enzyme. A reduction in the level of CAT activity was also observed in Panax ginseng (Ali et al, 2006) and C. roseus (Zhao et al, 2001), with the activities of these enzymes reverting back to normal levels 6 days after elicitation.…”

Section: Resultsmentioning

confidence: 77%

Effect of methyl jasmonate on isoflavonoid accumulation and antioxidant enzymes in Pueraria mirifica cell suspension culture

Saisavoey¹,

Thongchul²,

Sangvanich³

et al. 2014

J. Med. Plants Res.

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The effect of methyl jasmonate (MJ) on the isoflavonoid accumulation and activities of three antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) were studied in Pueraria mirifica cell suspension culture after MJ elicitation for up to 6 days. Treatment with MJ at a concentration of 1.0 µg/ml induced the highest isoflavonoid contents at the first day after elicitation, when compared to the control. This optimum concentration of MJ induced an oxidative stress in P. mirifica cells. The activity levels of superoxide dismutase and glutathione peroxidase were both increased, whilst that for catalase was decreased in the MJ-treated cells. Thus, P. mirifica cells have a defense mechanism against superoxide and hydrogen peroxide stress and MJ is one such potential stimulator of this defense mechanism.

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“…Interestingly, we found the increased levels of POD were closely associated with accumulation of TIAs, including VIN, VBL and CAT under the early drought stress. Previous reports showed that the abiotic stresses can increase the alkaloid content in plants by treatment of salinity, various chemicals and bioregulators (Zhao et al 2001;Karadge & Gaikwad 2003;Misra & Gupta 2006).…”

Section: Discussionmentioning

confidence: 99%

Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in Catharanthus roseus

Liu

Meng

Xi-Hua

et al. 2017

Journal of Plant Interactions

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Catharanthus roseus (C. roseus) plants were used to investigate the terpenoid indole alkaloids (TIAs) accumulation under the condition of PEG-induced drought stress. Multivariate analysis showed that 35% PEG6000 could induce more obvious and stable accumulation on proline (PRO) content and the relative water content (RWC). The results indicated that there were gradual increase and then decrease (p < .05) in the contents of vindoline (VIN) and catharanthine (CAT) under 35% PEG6000 stress, but the content of vinblastine (VBL) increased gradually. In addition, the expression levels of tryptophan decarboxylase (TDC), strictosidine synthase (STR) and deacetylvindoline-4-Oacetyltransferase (DAT) were upregulated in plants under 35% PEG6000 stress. Further correlation analysis indicated that CAT accumulation was significantly correlated with TDC gene expression, and VBL accumulation was significantly correlated with peroxidase (p < .05). Our results suggest that the cultivation of C. roseus in drought stress would serve as effective treatment for accumulating TIAs. ARTICLE HISTORY

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Elicitor-induced indole alkaloid biosynthesis in Catharanthus roseus cell cultures is related to Ca2+ influx and the oxidative burst

Cited by 88 publications

References 27 publications

Jasmonic acid elicits oxidative defense and detoxification systems in Cucumis melo L. cells

Jasmonic acid elicits oxidative defense and detoxification systems in Cucumis melo L. cells

Effect of methyl jasmonate on isoflavonoid accumulation and antioxidant enzymes in Pueraria mirifica cell suspension culture

Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in Catharanthus roseus

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