Elicitor activity of algino-oligosaccharide and its potential application in protection of rice plant (Oryza salivaL.) againstMagnaporthe grisea

Abstract

Rice blast caused by Magnaporthe grisea is considered to be one of the most devastating diseases in most rice-growing regions. In order to search for an effective agent to protect against rice infection, the elicitor activity of alginooligosaccharides was evaluated in germinating rice, in which accumulation of oryzalexin C could be detected sensitively. The maximal elicitor activity was detected in germinating rice seedlings when the fractions of alginate degradation products with a degree of polymerization of 7.9 (S3) and 6.5 (S4) were used as elicitors. Algino-oligosaccharides could induce the enzyme activities of phenylalanine ammonia lyase, peroxidase and catalase in rice plant cells for protection of the plant against the invading pathogen. With utilization of algino-oligosaccharides, the disease index caused by Magnaporthe grisea could be reduced from 17.74% to 10.81% and the protection efficacy was 39.06%. These findings suggest that algino-oligosaccharides could be applied as a biological agent to control rice blast disease.

Oligo-Alginate with Low Molecular Mass Improves Growth and Physiological Activity of Eucomis autumnalis under Salinity Stress

“…Oligo-alginate may stimulate ROS detoxifying enzymes and enhance activity of isozymes responsible for stress protection. This hypothesis was confirmed by Zhang et al [ 77 ], who reported that algino-oligosaccharides induced the activity of phenylalanine ammonia lyase (PAL), peroxidase (POD) and catalase (CAT) in rice cells and protected plants against pathogens. Changes in the activity of antioxidant enzymes may most probably determine the anti-stress effect of oligo-alginate in plants, but this requires further research.…”

Formulation of Microbial Inoculants by Encapsulation in Natural Polysaccharides: Focus on Beneficial Properties of Carrier Additives and Derivatives

“…The positive effect on plants of seaweed crude extracts, the raw material from which several polysaccharides used for encapsulation technology derive, is based on the synergic action of growth regulators, osmolytes, polysaccharides and other algal compounds (Battacharyya et al, 2015). Seaweeds are known for their action as bioelicitors and particularly laminarin, carrageenan, and alginate, have been studied for their plant defense stimulating effects (Chandía et al, 2004;Khan et al, 2009;El Modafar et al, 2012;Vera et al, 2012;Zhang et al, 2015;Abouraicha et al, 2017;Ben Salah et al, 2018). There are strong evidences that polysaccharides play an important role in the mechanisms of abiotic stress protection for microorganisms (Vassilev et al, 2012).…”

Glycans as Modulators of Plant Defense Against Filamentous Pathogens

“…Most oligosaccharides implicated in plant–pathogen interactions are generated by the enzymatic degradation of polysaccharides from the structural constituents of fungal cell wall or pathogen virulence factors ( Zhang S. et al, 2015 ). The activity of these oligosaccharides is highly dependent on their degree of polymerization (DP) ( Trouvelot et al, 2014 ).…”

“…Alginate oligomers are gaining increasing attention as new elicitor materials for inducing plant defense machinery by stimulating the accumulation of phytoalexin and inducing PAL activity in soybean cotyledons ( An et al, 2009 ). Furthermore, alginate oligosaccharides rich in polymannuronate are reported to activate defense responses in O. sativa against M. oryzae by inducing increased level of PAL, phytoalexin production, and POX activity ( Zhang S. et al, 2015 ).…”