Eliciting Targeted Mutations in Medicago sativa Using CRISPR/Cas9-Mediated Genome Editing: A Potential Tool for the Improvement of Disease Resistance

Subedi, Udaya; Hughes, Kimberley Burton; Chen, Guanqun; Hannoufa, Abdelali; Singer, Stacy D.

doi:10.1007/978-1-0716-3159-1_16

Cited by 1 publication

(1 citation statement)

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“…The investigation into CRISPR/Cas9-mediated genome editing's potential to enhance disease resistance in alfalfa underscored its value as a precise genetic alteration tool in breeding. The procedures outlined for generating a single nonhomologous end-joining-derived indel at a targeted genomic locus demonstrate the method's precision and efficiency [57]. In a related study, CRISPR/Cas9 was used to knock out the isoflavone synthase gene in red clover, leading to significant reductions in isoflavone concentrations, without impacting nodulation, indicating its potential role in rhizosphere defense over rhizobial signaling [58].…”

Section: Genome Editingmentioning

confidence: 99%

Advances in Molecular Breeding of Forage Crops: Technologies, Applications and Prospects

Chen

2024

Agriculture

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Molecular breeding has revolutionized the improvement of forage crops by offering precise tools to enhance the yield, quality, and environmental resilience. This review provides a comprehensive overview of the current technologies, applications, and future directions in the field of forage crop molecular breeding. Technological advancements in the field, including Quantitative Trait Loci (QTL) mapping, Genome-Wide Association Studies (GWASs), genomic selection (GS), and genome-editing tools such as CRISPR-Cas9, have significantly advanced the identification and incorporation of beneficial traits into forage species. These approaches have dramatically shortened the breeding cycles and increased the efficiency of developing cultivars with improved yield, disease resistance, stress tolerance, and nutritional profiles. The implementation of these technologies has led to notable successes, as demonstrated by case studies on various forage crops, showcasing enhanced forage quality and adaptability to challenging environmental conditions. Furthermore, the integration of high-throughput phenotyping with advanced bioinformatics tools has streamlined the management of large-scale genomic data, facilitating more precise selection and breeding decisions. Looking ahead, this review explores the potential of emerging technologies, such as the application of artificial intelligence in predictive breeding, along with the associated ethical and regulatory considerations. While we stand to gain benefit from these innovations, the future of molecular breeding in forage crops must also confront the challenges posed by climate change and the imperative of sustainable agricultural practices. This review concludes by emphasizing the transformative impact of molecular breeding on the improvement of forage crop and the critical need for ongoing research and collaboration to fully realize its potential.

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Section: Genome Editingmentioning

confidence: 99%

Advances in Molecular Breeding of Forage Crops: Technologies, Applications and Prospects

Chen

2024

Agriculture

View full text Add to dashboard Cite

show abstract

Eliciting Targeted Mutations in Medicago sativa Using CRISPR/Cas9-Mediated Genome Editing: A Potential Tool for the Improvement of Disease Resistance

Cited by 1 publication

References 43 publications

Advances in Molecular Breeding of Forage Crops: Technologies, Applications and Prospects

Advances in Molecular Breeding of Forage Crops: Technologies, Applications and Prospects

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