Eliciting and Analyzing Male Mouse Ultrasonic Vocalization (USV) Songs

Chabout, Jonathan; Jones-Macopson, Joshua; Jarvis, Erich D.

doi:10.3791/54137-v

Cited by 5 publications

(7 citation statements)

References 13 publications

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“…The thresh old of syllable frequency is from 35 kHz to 110 kHz. Syllable repertoire composition was classified into 4 categories: 1) simple syllables "s" only containing one note; 2) downward syllables "d" containing two notes that from a higher frequency note jumps to a lower frequency note; 3) upward "u" containing two notes that from a lower frequency note jumps to a higher frequency note; 4) multiple syllables "m" containing three or more notes by Mouse Song Analyzed v1.3 [68]. We termed syllable transition as one syllable to another syllable, like "s" to "s", "s" to "d", "m" to "s", and so on.…”

Section: Mouse Behavior Analysismentioning

confidence: 99%

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TCF7L2 acts as a molecular switch in midbrain to control mammal vocalization through its DNA binding domain but not transcription activation domain

Luo

et al. 2023

Mol Psychiatry

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Vocalization is an essential medium for social signaling in birds and mammals. Periaqueductal gray (PAG) a conserved midbrain structure is believed to be responsible for innate vocalizations, but its molecular regulation remains largely unknown. Here, through a mouse forward genetic screening we identified one of the key Wnt/β-catenin effectors TCF7L2/TCF4 controls ultrasonic vocalization (USV) production and syllable complexity during maternal deprivation and sexual encounter. Early developmental expression of TCF7L2 in PAG excitatory neurons is necessary for the complex trait, while TCF7L2 loss reduces neuronal gene expressions and synaptic transmission in PAG. TCF7L2-mediated vocal control is independent of its β-catenin-binding domain but dependent of its DNA binding ability. Patient mutations associated with developmental disorders, including autism spectrum disorders, disrupt the transcriptional repression effect of TCF7L2, while mice carrying those mutations display severe USV impairments. Therefore, we conclude that TCF7L2 orchestrates gene expression in midbrain to control vocal production through its DNA binding but not transcription activation domain.

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Section: Mouse Behavior Analysismentioning

confidence: 99%

“…We termed syllable transition as one syllable to another syllable, like "s" to "s", "s" to "d", "m" to "s", and so on. Syllable transitions of conditional probability for each context were analyzed by a customized script generated in Microsoft Excel [68].…”

Section: Mouse Behavior Analysismentioning

confidence: 99%

TCF7L2 acts as a molecular switch in midbrain to control mammal vocalization through its DNA binding domain but not transcription activation domain

Luo

et al. 2023

Mol Psychiatry

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“…For the USV recordings, a novel age‐matched B6 female mouse was used to stimulate USV emissions in each test animal. To avoid USV production by B6 mice, we anaesthetised them using an intraperitoneal (IP) injection of pentobarbital (50 mg/kg body weight pentobarbital [Sigma‐Aldrich]) and kept them in the home cage of the test animal (Figure 1D), as described in previous studies 40,41 . The test was performed between 14:00 and 17:00.…”

Section: Methodsmentioning

confidence: 99%

Association of tameness and sociability but no sign of domestication syndrome in mice selectively bred for active tameness

Venkatachalam,

Biswa,

Nagayama

et al. 2024

Genes Brain and Behavior

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Domesticated animals have been developed by selecting desirable traits following the initial unconscious selection stage, and now exhibit phenotypes desired by humans. Tameness is a common behavioural trait found in all domesticated animals. At the same time, these domesticated animals exhibit a variety of morphological, behavioural, and physiological traits that differ from their wild counterparts of their ancestral species. These traits are collectively referred to as domestication syndrome. However, whether this phenomenon exists is debatable. Previously, selective breeding has been used to enhance active tameness, a motivation to interact with humans, in wild heterogeneous stock mice derived from eight wild inbred strains. In the current study, we used tame mice to study how selective breeding for active tameness affects behavioural and morphological traits. A series of behavioural and morphological analyses on mice showed an increased preference for social stimuli and a longer duration of engagement in non‐aggressive behaviour. However, no differences were observed in exploratory or anxiety‐related behaviours. Similarly, selection for tameness did not affect ultrasonic vocalisations in mice, and no changes were observed in known morphological traits associated with domestication syndrome. These results suggest that there may be a link between active tameness and sociability and provide insights into the relationship between tameness and other behaviours in the context of domestication.

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“…The MATLAB Mouse Song Analyzer analysis procedure has been previously described 17,18 . Specifically, vocalization .wav files were converted to sonograms using MATLAB syntax Comparison of Analysis Systems that was developed by the Jarvis lab (http://jarvislab.net/research/ mouse-vocal communication/).…”

Section: Matlab Mouse Song Analyzer Analysismentioning

confidence: 99%

“…One such program is the Mouse Song Analyzer (v. 1.3), developed by the Jarvis lab (http://jarvislab.net/research/mouse-vocal-communication/). The Mouse Song Analyzer is a widely available system used in numerous publications [17][18][19][20][21][22] . It is fully automated and extremely time efficient, as each file takes approximately 2-3 minutes to assess.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the DeepSqueak and Mouse Song Analyzer vocalization analysis systems in C57BL/6J, FVB.129, and FVB neonates

Binder

Pranske

Lugo

2021

Journal of Neuroscience Methods

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Eliciting and Analyzing Male Mouse Ultrasonic Vocalization (USV) Songs

Cited by 5 publications

References 13 publications

TCF7L2 acts as a molecular switch in midbrain to control mammal vocalization through its DNA binding domain but not transcription activation domain

TCF7L2 acts as a molecular switch in midbrain to control mammal vocalization through its DNA binding domain but not transcription activation domain

Association of tameness and sociability but no sign of domestication syndrome in mice selectively bred for active tameness

Evaluating the DeepSqueak and Mouse Song Analyzer vocalization analysis systems in C57BL/6J, FVB.129, and FVB neonates

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