Elicitin 172 from an isolate of Phytophthora nicotianae pathogenic to tomato

Capasso, Raffaele; Cristinzio, Gennaro; Evidente, Antonio; Visca, Carla; Ferranti, Pasquale; Blanco, F Del Vecchio; Parente, Augusto

doi:10.1016/s0031-9422(98)00539-1

Cited by 10 publications

(3 citation statements)

References 19 publications

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“…In addition, with the specific bioassay on tomato seedlings, the toxic activity was only found in the culture filtrates from P. cactorum and P. nicotianae; none was found from P. cinnamomi, P. cryptogea, P. capsici, P. citrophthora, or P. infestans. Furthermore, P. nicotianae, a well known tomato pathogen, from which other phytotoxic proteins have been characterized (18,35), lacked the PcF protein based on the PcF purification protocol. Therefore, the observed constitutive PcF protein expression and secretion from P. cactorum RACE, but not from other Phytophthora spp., leads us to hypothesize that PcF might be either unique for the P. cactorum, or, possibly, for those P. cactorum strains that are pathogenic to strawberry.…”

Section: Resultsmentioning

confidence: 99%

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Phytotoxic Protein PcF, Purification, Characterization, and cDNA Sequencing of a Novel Hydroxyproline-containing Factor Secreted by the Strawberry Pathogen Phytophthora cactorum

Orsomando¹,

Lorenzi²,

Raffaelli

et al. 2001

Journal of Biological Chemistry

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A novel protein factor, named PcF, has been isolated from the culture filtrate of Phytophthora cactorum strain P381 using a highly sensitive leaf necrosis bioassay with tomato seedlings. Isolated PcF protein alone induced leaf necrosis on its host strawberry plant. The primary structure and cDNA sequence of this novel phytotoxic protein was determined, and BLAST searches of Swiss-Prot, EMBL, and GenBank TM /EBI data banks showed that PcF shared no significant homology with other known sequences. The 52-residue PcF protein, which contains a 4-hydroxyproline residue along with three S-S bridges, exhibits a high content of acidic sidechains, accounting for its isoelectric point of 4.4. The molecular mass of isolated PcF is 5,622 ؎ 0.5 Da as determined by mass spectrometry and matches that calculated from the deduced amino acid sequence with cDNA sequencing. The cDNA sequence indicates that PcF is first produced as a larger precursor, comprising an additional N-terminal, 21-residue secretory signal peptide. Maturation of this protein involves the hydroxylation of proline 49, a feature that is unique among other known secreted fungal phytopathogenic proteins.In modern agriculture, the selection of pathogen-resistant cultivars remains of the utmost importance. Conventional breeding selection protocols are relatively inefficient as a consequence of the general lack of genetic variability in cultivated plants as well as the inability to keep pace with the rapid adaptation of pathogen genotypes. New strategies, aimed at achieving resistant plants through gene engineering, require an in depth knowledge of the mechanism of pathogenesis at the molecular level. Host-pathogen interactions can result in either "incompatibility" (resistance) or "susceptibility" (pathogenesis). In either case, mounting evidence suggests that the process is mediated by the production of so-called elicitor-and toxinsignaling molecules (1-3). Signal recognition at the plant cell surface triggers an ordered cascade of downstream events, leading to a range of host-cell responses (4 -7). Because elicitor and toxic mediators play a central role, elucidation of the mechanism of plant-pathogen interactions promises to provide insights about strategies for incorporating pathogen resistance in cultivated plants.The European cultivated strawberry plants (Fragaria vesca ϫ ananassa Duch.) are mostly susceptible to attack by Phytophthora cactorum (8, 9), a pathogenic oomycete for many herbaceous and woody plants because of its wide range host specificity. This pathogen is the causal agent of the "leather rot" and "root rot" diseases in strawberry plants, whose morphological symptoms are recognized by rotting of root, crown, and fruit tissues (10). Plich and Rudnicki (11) first reported that culture filtrates of P. cactorum possessed phytotoxins with action on tomato and involvement in the development of apple tree diseases. Most of these metabolites, however, have not been purified and characterized. The exception is "cactorein" from P. cactorum, a secreted pr...

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Section: Resultsmentioning

confidence: 99%

“…Bioassays-Phytotoxic activity of either crude culture filtrate or purified fractions, was routinely assayed on tomato seedlings (18). Tomato seedlings (Lycopersicon esculentum cv.…”

Section: Methodsmentioning

confidence: 99%

Phytotoxic Protein PcF, Purification, Characterization, and cDNA Sequencing of a Novel Hydroxyproline-containing Factor Secreted by the Strawberry Pathogen Phytophthora cactorum

Orsomando¹,

Lorenzi²,

Raffaelli

et al. 2001

Journal of Biological Chemistry

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“…The M. oryzae genome harbors 19 proteins with the CFEM domain (Kou et al, 2017). Two CFEM-containing proteins Wang et al, 2003;Zhang et al, 2004; Billard et al, 1988;Huet and Pernollet, 1989 P. colocasiae † 15-kDa glycoprotein Elicitin ND Induces cell death and SAR in taro leaf Mishra et al, 2009 P. cryptogea † Cryptogein Elicitin ND Induces cell death, SAR and defense of tobacco against P. nicotianae Ricci et al, 1989;Galiana et al, 1997;Mikes et al, 1997;Leborgne-Castel et al, 2008;Coursol et al, 2015;Kulik et al, 2015;Ptáčková et al, 2015;Starý et al, Huet et al, 1994;Kamoun et al, 1997Kamoun et al, , 1998Kanzaki et al, 2003;Huitema et al, 2005;Du et al, 2015 (Continued) Frontiers in Genetics | www.frontiersin.org Mateos et al, 1997;Khatib et al, 2004 P. parasitica † PpNLP/NLP Pp NLP ND Induces cell death; carries a nlp20 pattern recognized by RLP23 which associates with SOBIR1 and BAK1 complex to trigger immune responses Fellbrich et al, 2002;Qutob et al, 2006;Ottmann et al, 2009;Böhm et al, Kamoun et al, 1993;Mouton-Perronnet et al, 1995;Capasso et al, 1999 PPTG_02039, PPTG_14297, PPTG_09966 MoCDIP2 and MoCDIP11 have been identified to induce cell in the non-host N. benthamiana and host rice cells Guo et al, 2019). MoCDIP2 contains a CFEM domain at the N-terminus, and a GPI-anchored site at the C-terminus, whereas MoCDIP11 contains only a CFEM domain at the N-terminus.…”

Section: Major Protein Families Of Oomycete and Fungus Apoplastic CDImentioning

confidence: 99%

Apoplastic Cell Death-Inducing Proteins of Filamentous Plant Pathogens: Roles in Plant-Pathogen Interactions

Han

et al. 2020

Front. Genet.

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Filamentous pathogens, such as phytopathogenic oomycetes and fungi, secrete a remarkable diversity of apoplastic effector proteins to facilitate infection, many of which are able to induce cell death in plants. Over the past decades, over 177 apoplastic cell death-inducing proteins (CDIPs) have been identified in filamentous oomycetes and fungi. An emerging number of studies have demonstrated the role of many apoplastic CDIPs as essential virulence factors. At the same time, apoplastic CDIPs have been documented to be recognized by plant cells as pathogen-associated molecular patterns (PAMPs). The recent findings of extracellular recognition of apoplastic CDIPs by plant leucine-rich repeat-receptor-like proteins (LRR-RLPs) have greatly advanced our understanding of how plants detect them and mount a defense response. This review summarizes the latest advances in identifying apoplastic CDIPs of plant pathogenic oomycetes and fungi, and our current understanding of the dual roles of apoplastic CDIPs in plant-filamentous pathogen interactions.

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Signal Transduction in Host-Parasite Interactions

Huang

2001

Plant Pathogenesis and Resistance

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Elicitin 172 from an isolate of Phytophthora nicotianae pathogenic to tomato

Cited by 10 publications

References 19 publications

Phytotoxic Protein PcF, Purification, Characterization, and cDNA Sequencing of a Novel Hydroxyproline-containing Factor Secreted by the Strawberry Pathogen Phytophthora cactorum

Phytotoxic Protein PcF, Purification, Characterization, and cDNA Sequencing of a Novel Hydroxyproline-containing Factor Secreted by the Strawberry Pathogen Phytophthora cactorum

Apoplastic Cell Death-Inducing Proteins of Filamentous Plant Pathogens: Roles in Plant-Pathogen Interactions

Signal Transduction in Host-Parasite Interactions

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