Elicitation of antioxidant metabolites in Musa species in vitro shoot culture using sucrose, temperature and jasmonic acid

Abstract

In vitro culture is not only known for the mass propagation of medicinal plants, it can also be used to boost the accumulation of useful metabolites. This work was aimed at studying the potential of elicitation on the accumulation of antioxidant secondary metabolites in different Musa species accessions. Shoot cultures of selected six Musa spp. accessions were micropropagated on Murashige and Skoog mineral-based culture medium, supplemented with indole-3-acetic acid and 6-benzylaminopurine. Different temperatures (15, 20 and 26 °C), doses of sucrose (30–50 g/L) and jasmonic acid concentrations (JA, 50–200 µM) were used as elicitors in the culture medium. High-performance thin-layer chromatography (HPTLC) coupled with effect-directed analysis (EDA) was used to compare and characterize all the 71 Musa spp. samples from the selected accessions and to reveal compound zones with antioxidant, antidiabetic and anticholinesterase activities. All elicitation and plant tissue culture experiments increased the total phenolic content (TPC) and antioxidant activity of the selected Musa spp. accessions. Extracts from plants grown at 20 °C gave the highest TPC and antioxidant activity. Addition of JA (200 µM) and higher sucrose contents (40 g/L or 45 g/L) increased the TPC and antioxidant activity as well. Not only compounds with antioxidant, but also antidiabetic and anticholinesterase activities were detected in the elicited accessions. The system presented in this study could serve as a sustainable, consistent and reliable alternative means of bioactive metabolites supply for the production of biopharmaceuticals.

In vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of Paris polyphylla Smith: an important Himalayan medicinal plant

“…Minimum antioxidant activity was recorded with DPPH assay (1.09 mM Ascorbic Acid Equivalent/100 g of dry weight) and maximum was recorded for ABTS assay (6.89 mM Ascorbic Acid Equivalent/100 g of dry weight). Similar results have been reported in recent studies ( Jalalpour et al., 2014 ; Makowczyńska et al., 2015 ; Ayoola-Oresanya et al., 2021 ).…”

“…According to studies by Jalalpour et al. (2014) and Ayoola-Oresanya et al. (2021) , MeJA was responsible for increasing phenolic and flavonoid accumulation in Taxus baccata cell culture.…”

“…However, using the DPPH assay, no appreciable difference in antioxidant activity was found. Many studies have shown how different PGRs and elicitors can be used to produce or augment secondary metabolites and other advantageous bioactive compounds ( Giri et al., 2012 ; Jalalpour et al., 2014 ; Makowczyńska et al., 2015 ; Ayoola-Oresanya et al., 2021 ). The elicitor MeJA (50 µM concentration) was found to be quite successful at increasing the total phenol, flavonoids, and tannin content in P. polyphylla , whereas TDZ was useful in improving callus biomass.…”

Elicitation Induced α-Amyrin Synthesis in Tylophora indica In Vitro Cultures and Comparative Phytochemical Analyses of In Vivo and Micropropagated Plants

“…These plant secondary metabolites are now being extensively utilized in herbal, cosmetic and nutraceutical formulations [ 4 ]. However, the over-exploitation of plants has led to a drastic decline of biodiversity [ 5 ]. In vitro techniques propagate plants at a much faster rate with no risk of pathogens, and this method can be used as a reliable tool for the high yield of SMs [ 6 , 7 ].…”

Effects of two abiotic elicitors on secondary metabolites accumulation and bioactivity in tree wormwood i<i>n vitro</i> shoot cultures

“…The in vitro application of various types of elicitors including abiotic stress, nanoparticles, jasmonic acid, salicylic acid among others has been shown efficient to enhance the production of useful bioactive compounds belonging to the classes of terpenoids, phenolics, flavonoids, alkaloids in various plant species (Ayoola-Oresanya et al, 2021;Khan et al, 2021;Yazdanian et al, 2022). In this study, the effect of NaCl and KCl used as two abiotic elicitors on the concentration of phenolics and volatile metabolites in the in vitro regenerated microshoots of Artemisia arborescens L. was investigated.…”