Elicitation improves rosmarinic acid content and antioxidant activity in Thymus lotocephalus shoot cultures

Gonçalves, Sandra; Mansinhos, Inês; Rodríguez‐Solana, Raquel; Pérez‐Santín, Efrén; Coelho, Natacha

doi:10.1016/j.indcrop.2019.04.071

Cited by 37 publications

(41 citation statements)

References 52 publications

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“…These in vitro techniques have been widely used for the production of several SMs, notably taxol [ 31 , 32 , 33 ], podophyllotoxin [ 34 , 35 , 36 ], withanolides [ 37 ], centellosides [ 38 , 39 ], and rosmarinic acid (RA) [ 40 , 41 , 42 , 43 ], as well as for mass propagation [ 44 ], in vitro cloning [ 45 , 46 , 47 ], and polyploidization [ 48 , 49 ]. Many RA-producing biotechnological platforms have been established, based on shoots [ 50 , 51 , 52 ], callus [ 53 ], cells [ 41 , 42 , 54 ], and hairy root cultures [ 55 , 56 , 57 , 58 ] of numerous species of the Boraginaceae, Anthocerotaceae, and Lamiaceae.…”

Section: Introductionmentioning

confidence: 99%

Powerful Plant Antioxidants: A New Biosustainable Approach to the Production of Rosmarinic Acid

et al. 2020

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Modern lifestyle factors, such as physical inactivity, obesity, smoking, and exposure to environmental pollution, induce excessive generation of free radicals and reactive oxygen species (ROS) in the body. These by-products of oxygen metabolism play a key role in the development of various human diseases such as cancer, diabetes, heart failure, brain damage, muscle problems, premature aging, eye injuries, and a weakened immune system. Synthetic and natural antioxidants, which act as free radical scavengers, are widely used in the food and beverage industries. The toxicity and carcinogenic effects of some synthetic antioxidants have generated interest in natural alternatives, especially plant-derived polyphenols (e.g., phenolic acids, flavonoids, stilbenes, tannins, coumarins, lignins, lignans, quinines, curcuminoids, chalcones, and essential oil terpenoids). This review focuses on the well-known phenolic antioxidant rosmarinic acid (RA), an ester of caffeic acid and (R)-(+)-3-(3,4-dihydroxyphenyl) lactic acid, describing its wide distribution in thirty-nine plant families and the potential productivity of plant sources. A botanical and phytochemical description is provided of a new rich source of RA, Satureja khuzistanica Jamzad (Lamiaceae). Recently reported approaches to the biotechnological production of RA are summarized, highlighting the establishment of cell suspension cultures of S. khuzistanica as an RA chemical biofactory.

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Section: Introductionmentioning

confidence: 99%

Powerful Plant Antioxidants: A New Biosustainable Approach to the Production of Rosmarinic Acid

et al. 2020

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“…In addition to the main anti-cancer alkaloids, which have been presented above, a number of other terpenes, or phenolic compounds, which are common in many other plant species of the Lamiaceae, Asteracea and Fabaceae families, among others, are of potential value. Many such compounds are known to possess various anti-inflammatory, anticancer, antioxidant, antidiabetic, hepatoprotective or antimicrobial properties [15][16][17][18][19][20][21][22][23]. Of these, the antibacterial properties have drawn significant interest due to the growing problem of bacterial infections around the world [92,93].…”

Section: Overproduction Of Other Secondary Metabolites In Transgenic mentioning

confidence: 99%

“…Secondary metabolites can affect cancer cells by interfering with their division, and by changing their metabolism and even the expression of selected genes. Many also have antioxidant [15,16], anti-inflammatory [17,18], antibacterial [19], antifungal [20,21], neurological [22] or hepatoprotective [23] effects. As plants represent such an important source of many secondary metabolites, there is great interest in increasing their biosynthetic rates as part of green biotechnology, which includes the use of transgenic plants or other photosynthetic organisms for industrial purposes.…”

Section: Introductionmentioning

confidence: 99%

Transgenesis as a Tool for the Efficient Production of Selected Secondary Metabolites from Plant in Vitro Cultures

Kowalczyk

Wieczfińska

Skała

et al. 2020

Plants

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The plant kingdom abounds in countless species with potential medical uses. Many of them contain valuable secondary metabolites belonging to different classes and demonstrating anticancer, anti-inflammatory, antioxidant, antimicrobial or antidiabetic properties. Many of these metabolites, e.g., paclitaxel, vinblastine, betulinic acid, chlorogenic acid or ferrulic acid, have potential applications in medicine. Additionally, these compounds have many therapeutic and health-promoting properties. The growing demand for these plant secondary metabolites forces the use of new green biotechnology tools to create new, more productive in vitro transgenic plant cultures. These procedures have yielded many promising results, and transgenic cultures have been found to be safe, efficient and cost-effective sources of valuable secondary metabolites for medicine and industry. This review focuses on the use of various in vitro plant culture systems for the production of secondary metabolites.

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“…It is one of the most common natural elicitors used in in vitro culture to induce secondary metabolites production (Cheng et al 2013 ; Karalija et al 2020 ). It has been used also successfully in various studies to improve the production of secondary metabolites such as rosmarinic acid (Gonçalves et al 2019 ), sanguinarine (Guízar-González et al 2016 ), xanthone (Krstić-Milošević et al 2017 ) isoflavonoid (Rani et al 2020 ), plumbagin (Singh et al 2020 ) and astragalosides (Park et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology

Farjaminezhad

Garoosi

2021

AMB Expr

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Neem is a medicinal plant used as antimalarial, antibacterial, antiviral, insecticide, and antimicrobial drug. This study aimed to investigate and predict the effect of yeast extract and sampling time on cell growth, secondary metabolites synthesis, SQS1 and MOF1 genes expression by response surface methodology. The highest fresh and dry cell weights were 580.25 g/L and 21.01 g/L, respectively obtained 6 days after using 100 mg/L yeast extract. The highest azadirachtin accumulation and production were 16.08 mg/g DW and 219.78 mg/L obtained 2 and 4 days, respectively after using 25 mg/L yeast extract. Maximum mevalonic acid accumulation (1.75 mg/g DW) and production (23.77 mg/L) were observed 2 days after application of 50 mg/L yeast extract. The highest amount of squalene accumulation (0.22 mg/g DW) and production (4.53 mg/L) were achieved 4 days after using 50 mg/L yeast extract. Prediction results exhibited the highest azadirachtin accumulation (13.61 mg/g DW) and production (190.50 mg/L), mevalonic acid accumulation (0.50 mg/g DW) and production (5.57 mg/L), and squalene accumulation (0.30 mg/g DW) by using 245 mg/L yeast extract for 2 days, 71 mg/L yeast extract for 2 days, 200 mg/L yeast extract for 4.96 days, without yeast extract for 6.54 days and 4 days, respectively. Also, it was predicted that the highest squalene production is achieved by long-term exposure to high concentrations of yeast extract. The qRT-PCR analysis displayed the maximum relative gene expression of SQS1 and MOF1 by using 150 and 25 mg/L yeast extract for 4 and 2 days treatment.

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Elicitation improves rosmarinic acid content and antioxidant activity in Thymus lotocephalus shoot cultures

Cited by 37 publications

References 52 publications

Powerful Plant Antioxidants: A New Biosustainable Approach to the Production of Rosmarinic Acid

Powerful Plant Antioxidants: A New Biosustainable Approach to the Production of Rosmarinic Acid

Transgenesis as a Tool for the Efficient Production of Selected Secondary Metabolites from Plant in Vitro Cultures

Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology

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