Elicitation Enhanced the Production of Phenolic Compounds and Biological Activities in Hairy Root Cultures of Bitter melon ( Momordica charantia L.)

Chung, Ill‐Min; Thiruvengadam, Muthu; Rekha, Kaliyaperumal; Rajakumar, Govindasamy

doi:10.1590/1678-4324-2016160393

Cited by 29 publications

(11 citation statements)

References 34 publications

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“…Overall, elicitation with MeJA caused significant increases in sinapic, ferulic, and caffeic acids and in quercetin (Figure 3). This increased production of phenol derivates was also obtained when applying elicitors to roots of bitter melon (Chung et al, 2016) and stevia plants (González-Chavira et al, 2018). Finally, sterile filtrates of Verticillum non-alfalfae significantly increased PAL activity in hop cell lines (Kunej et al, 2020).…”

Section: Discussionmentioning

confidence: 64%

Effect of Methyl Jasmonate in Gene Expression, and in Hormonal and Phenolic Profiles of Holm Oak Embryogenic Lines Before and After Infection With Phytophthora cinnamomi

et al. 2022

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The dieback syndrome affecting Quercus ilex and other oak species impels the search for tolerant plant genotypes, as well as methods of plant immunization against such infections. Elicitation treatments can be an effective strategy to activate plant defense response and embryogenic lines represent a promising tool to generate new tolerant genotypes and also to study early markers involved in defense response. The aim of the presented work was to investigate changes in gene expression, and in hormonal and phenolic profiles induced in three holm oak embryogenic lines (ELs) elicited with methyl jasmonate (MeJA) before and after infection with the oomycete Phytophthora cinnamomi, which is the main biotic agent involved in this pathogenic process. The three ELs, derived from three genotypes, showed different basal profiles in all tested parameters, noting that the VA5 naïve genotype from a scape tree was characterized by a basal higher expression in NADPH-dependent cinnamyl alcohol dehydrogenase (CAD) and chalcone synthase (CHS) genes and also by higher caffeic acid content. Our work also identifies changes triggered by MeJA elicitation in holm oak embryogenic lines, such as increases in ABA and JA contents, as well as in levels of most of the determined phenolic compounds, especially in caffeic acid in Q8 and E00 ELs, but not in their biosynthesis genes. Irrespective of the EL, the response to oomycete infection in holm oak elicited plant material was characterized by a further increase in JA. Since JA and phenols have been described as a part of the Q. ilex defense response against P. cinnamomi, we propose that MeJA may act as an induced resistance (IR) stimulus and that in our embryogenic material induced both direct (detected prior to any challenge) and primed (detected after subsequent challenge) defense responses.

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Section: Discussionmentioning

confidence: 64%

Effect of Methyl Jasmonate in Gene Expression, and in Hormonal and Phenolic Profiles of Holm Oak Embryogenic Lines Before and After Infection With Phytophthora cinnamomi

et al. 2022

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“…In this respect, Ismail et al 88 , found that total flavonoid and phenolic contents were higher in hairy root cultures of Lactuca sativa as compared to the non-transformed ones. Hairy root cultures of Momordica charantia were elicited with jasmonic acid and salicylic acid to enhance biomass accumulation and phenolic compound production 89 . The rolB gene was introduced in Vitis amurensis cells and the rolB-transformed calli are able of producing up to 3.15% dry weight of resveratrol 90 .…”

Section: Enhancementmentioning

confidence: 99%

In vitro Production of Plant Nutraceuticals

Bekheet¹,

Sota²

2022

Biotechnology

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Biotechnology methods particularly tissue culture techniques offer new approaches for the in vitro production of valuable plant nutraceuticals. The large-scale production of secondary metabolites in a year-round system without seasonal constraints is one of the advantages of plant tissue culture techniques. Furthermore, enhancement of in vitro nutraceuticals production can be realized by a selection of high-producing cell lines and medium optimization. In this respect, several techniques have been adopted to improve the production of plant-derived nutraceuticals such as mutation, elicitation, precursor feeding, genetic transformation and metabolic engineering. In vitro mutagenesis of cultured cells and tissues by irradiation or chemical mutagens represents a feasible method for the improvement of nutraceuticals production. Likewise, transformation is another strategy that is used to enhance nutraceuticals in plant cells or organ tissues. At this point, hairy root culture induced by Agrobacterium rhizogenes is the most promising transformation technique used for in vitro production of valuable plant compounds. The benefits of hairy roots are high metabolite productivity, high growth rates and inherent genetic stability. On the other hand, due to its effectiveness and practical feasibility, elicitation is considered the most applied strategy for enhancing the production of desired compounds in plant biotechnology. Biotic (biological origin) and abiotic (non-biological origin) elicitors are used to increase the production of plant metabolites by different effects on the cellular processes in the plant system. Furthermore, bioreactors developed for in vitro industrial-scale production of desired compounds can be used for continuous and scaling-up plant nutraceuticals production. This article discusses different aspects of biotechnology used for the selection and enhancement production of nutraceuticals from plant cultures grown cells, in vitro.

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“…In cucurbits, many works have demonstrated the valuable production of bioactive compounds in tissue culture. In C. anguria, M. charantia, and M. dioica, the production of phenolic compounds in transgenic and nontransgenic roots was demonstrated to be efficient, with the production being higher in transgenic versions of the three species [254][255][256][257]. In vitro cultures with jasmonic and salicilyc acids enhanced polyphenol production in M. charantia [258].…”

Section: Tissue Culturementioning

confidence: 99%

Genetic and Pre- and Postharvest Factors Influencing the Content of Antioxidants in Cucurbit Crops

2021

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Cucurbitaceae is one of the most economically important plant families, and includes some worldwide cultivated species like cucumber, melons, and squashes, and some regionally cultivated and feral species that contribute to the human diet. For centuries, cucurbits have been appreciated because of their nutritional value and, in traditional medicine, because of their ability to alleviate certain ailments. Several studies have demonstrated the remarkable contents of valuable compounds in cucurbits, including antioxidants such as polyphenols, flavonoids, and carotenoids, but also tannins and terpenoids, which are abundant. This antioxidant power is beneficial for human health, but also in facing plant diseases and abiotic stresses. This review brings together data on the antioxidant properties of cucurbit species, addressing the genetic and pre- and postharvest factors that regulate the antioxidant content in different plant organs. Environmental conditions, management, storage, and pre- and postharvest treatments influencing the biosynthesis and activity of antioxidants, together with the biodiversity of this family, are determinant in improving the antioxidant potential of this group of species. Plant breeding, as well as the development of innovative biotechnological approaches, is also leading to new possibilities for exploiting cucurbits as functional products.

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Elicitation Enhanced the Production of Phenolic Compounds and Biological Activities in Hairy Root Cultures of Bitter melon ( Momordica charantia L.)

Abstract: Momordica charantia (Cucurbitaceae) is an important vegetable and also medicinal crop which produces the bioactive compounds for various biological activities with potential uses in human health DPPH (84 and 78%), reducing potential (0.53 and 0.48), phosphomolybdenum (3.6 and 3.2 mg/g)

Cited by 29 publications

References 34 publications

Effect of Methyl Jasmonate in Gene Expression, and in Hormonal and Phenolic Profiles of Holm Oak Embryogenic Lines Before and After Infection With Phytophthora cinnamomi

Effect of Methyl Jasmonate in Gene Expression, and in Hormonal and Phenolic Profiles of Holm Oak Embryogenic Lines Before and After Infection With Phytophthora cinnamomi

In vitro Production of Plant Nutraceuticals

Genetic and Pre- and Postharvest Factors Influencing the Content of Antioxidants in Cucurbit Crops

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