Eleven microsatellite loci developed from the Chinese Hesperid butterflies, Polytremis fukia (Lepidoptera: Hesperiidae), and cross-amplification in other species of Polytremis

Molecular Phylogeny and Taxonomy of Lepidoptera with Special Reference to Influence of Wolbachia Infection in the Genus Polytremis

“…Comparisons of pair-wise F ST among six populations show the genetic difference based on 12 microsatellite loci (Table 4). The population of Baishanzu showed the largest pair-wise F ST values, corresponding to relatively geographic isolation from the other five populations [33].…”

“…Observed (HO) and expected heterozygosity (HE) values ranged from 0.33 to 0.71 and from 0.61 to 0.90. Only one locus (Polynyas 13) (p < 0.01) that deviated from HWE showed significant heterozygote deficit in the populations of Lianglu, Shengtangshan, and Maoershan [33]. Analysis performed with Micro-Checker showed that the deviation was attributed to the homozygote excess with null allele frequency of 0.2041, 0.2914, and 0.3533 in the three populations, respectively [33].…”

“…Only one locus (Polynyas 13) (p < 0.01) that deviated from HWE showed significant heterozygote deficit in the populations of Lianglu, Shengtangshan, and Maoershan [33]. Analysis performed with Micro-Checker showed that the deviation was attributed to the homozygote excess with null allele frequency of 0.2041, 0.2914, and 0.3533 in the three populations, respectively [33]. No linkage disequilibrium was detected for any pair of loci (p > 0.01) in any populations following Bonferroni correction [33].…”

“…Eight PCGs (ND2, ATP8, ATP6, COIII, ND3, ND4, ND6, and ND1) employ the typical stop codon TAA, while the remaining five PCGs terminate with TA or T [22]. Alignment of amino acid sequences of each of individual 13 PCGs was performed through Clustal X [32], and the phylogenetic analysis was carried out using neighbor-joining (NJ) method with MEGA version 5.0 program [33] (Figure 5).…”

“…Analysis performed with Micro-Checker showed that the deviation was attributed to the homozygote excess with null allele frequency of 0.2041, 0.2914, and 0.3533 in the three populations, respectively [33]. No linkage disequilibrium was detected for any pair of loci (p > 0.01) in any populations following Bonferroni correction [33]. Comparisons of pair-wise F ST among six populations show the genetic difference based on 12 microsatellite loci (Table 4).…”