Elevation of the Thromboxane A2/Prostacyclin Ratio in Urine of Diabetic Mice Analyzed by Gas Chromatography/Selected Ion Monitoring

Hishinuma, Takanori; Koseki, Yuko; Murai, Yuriko; Kotake, Jiro; Ishii, Fumio; Suzuki, Kenichi; Mizugaki, Michinao

doi:10.1016/s0090-6980(98)00012-4

Cited by 13 publications

(1 citation statement)

References 29 publications

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“…The prostanoid TXA 2 , belonging to the family of thromboxanes (TX), is recognized to have some pro‐aggregatory, vasoconstrictory and bronchoconstrictory effects 3–5. On the opposite side, PGI 2 , a member of the prostaglandins (PG) family, has anti‐aggregatory and vasodilatatory effects 6…”

Section: Introductionmentioning

confidence: 99%

Setting up a 2D‐LC/MS/MS method for the rapid quantitation of the prostanoid metabolites 6‐oxo‐PGF_1α and TXB₂ as markers for hemostasis assessment

Casetta¹,

Vecchione

Tomaiuolo

et al. 2008

J. Mass Spectrom.

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6-oxo-PGF(1alpha) and TXB(2) are the metabolites of the prostanglandin PGI(2) and of the thromboxane TXA(2), respectively. PGI(2) and TXA(2) are arachidonic acid-derived compounds which regulate the blood hemostasis. Their quick metabolism leads to the 6-oxo-PGF(1alpha) and TXB(2) metabolites in plasma. In order to study on a large base the external factors influencing the hemostatic conditions, there is a need for a fast and reliable assay for quantitating these metabolites. Some methods have been published for the analysis of the arachidonic acid-derived compounds and some are dealing with mass spectrometry but nonspecifically centered on these specific compounds with a fast and cheap protocol, amenable for large-scale studies. Here we describe an analytical strategy that incorporates a two-dimensional chromatography running coupled to tandem mass spectrometry that minimizes the sample preparation and addresses the presence of the TXB(2) anomers for a robust quantitation measurement. After a protein precipitation, 100 microl of the supernatant (corresponding to 50 microl of the original plasma) was injected in a two-dimensional chromatographic system which operates an on-line clean-up and a subsequent chromatographic separation of the targeted analytes with a limit of quantitation (LOQ) of 22 pg/ml for 6-oxo-PGF(1alpha), and and a LOQ of 25 pg/ml for TXB(2).

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Section: Introductionmentioning

confidence: 99%

Setting up a 2D‐LC/MS/MS method for the rapid quantitation of the prostanoid metabolites 6‐oxo‐PGF_1α and TXB₂ as markers for hemostasis assessment

Casetta¹,

Vecchione

Tomaiuolo

et al. 2008

J. Mass Spectrom.

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Simultaneous quantification of seven prostanoids using liquid chromatography/tandem mass spectrometry: The effects of arachidonic acid on prostanoid production in mouse bone marrow-derived mast cells

Hishinuma

Suzuki

Saito

et al. 2007

Prostaglandins, Leukotrienes and Essential Fatty Acids

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Troglitazone has a reducing effect on thromboxane production

Hishinuma

Yamazaki

Mizugaki

2000

Prostaglandins & Other Lipid Mediators

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Elevation of the Thromboxane A2/Prostacyclin Ratio in Urine of Diabetic Mice Analyzed by Gas Chromatography/Selected Ion Monitoring

Cited by 13 publications

References 29 publications

Setting up a 2D‐LC/MS/MS method for the rapid quantitation of the prostanoid metabolites 6‐oxo‐PGF_1α and TXB₂ as markers for hemostasis assessment

Setting up a 2D‐LC/MS/MS method for the rapid quantitation of the prostanoid metabolites 6‐oxo‐PGF_1α and TXB₂ as markers for hemostasis assessment

Simultaneous quantification of seven prostanoids using liquid chromatography/tandem mass spectrometry: The effects of arachidonic acid on prostanoid production in mouse bone marrow-derived mast cells

Troglitazone has a reducing effect on thromboxane production

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Elevation of the Thromboxane A2/Prostacyclin Ratio in Urine of Diabetic Mice Analyzed by Gas Chromatography/Selected Ion Monitoring

Cited by 13 publications

References 29 publications

Setting up a 2D‐LC/MS/MS method for the rapid quantitation of the prostanoid metabolites 6‐oxo‐PGF1α and TXB2 as markers for hemostasis assessment

Setting up a 2D‐LC/MS/MS method for the rapid quantitation of the prostanoid metabolites 6‐oxo‐PGF1α and TXB2 as markers for hemostasis assessment

Simultaneous quantification of seven prostanoids using liquid chromatography/tandem mass spectrometry: The effects of arachidonic acid on prostanoid production in mouse bone marrow-derived mast cells

Troglitazone has a reducing effect on thromboxane production

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Setting up a 2D‐LC/MS/MS method for the rapid quantitation of the prostanoid metabolites 6‐oxo‐PGF_1α and TXB₂ as markers for hemostasis assessment

Setting up a 2D‐LC/MS/MS method for the rapid quantitation of the prostanoid metabolites 6‐oxo‐PGF_1α and TXB₂ as markers for hemostasis assessment