Summary:We have evaluated a recently introduced colour test kit for the determination of serum angiotensin I-converting enzyme catalytic activity. p-Hydroxyhippuric acid, liberated from p-hydroxyhippuryl-L-histidyl-L-leucine by angiotensin I-converting enzyme, is cpnverted into a quinoneimine dye with an absorption maximum at 505 nm. The procedure shows excellent linearity over the whole ränge of catalytic activities found in serum. Intra-and inter-assay coefficients of Variation are 2-5 and 7-10% respectively. Correlation with a modified Cushman-Cheung ((1971) Biochem. Pharmacol. 20, 1637-1648) method currently used in our laboratory is good, with r = 0.985 and a regression equation of y (colour kit) = 0.423 (CushmanCheung) + 0.765. Haemoglobin, lipids, bilirubin and prednisone do not interfere but uric acid in concentrations higher than 600 / does. No extraction step is required. The assay is very rapid, and more than twenty samples can be determined in an hour.
Bewertende Prüfung eines Testbestecks zur schnellen und einfachen kolorimetrischen Bestimmung von Angiotensin I-Converting Enzyme im Serum