Elevation of Plasma TGF-β1 During Radiation Therapy Predicts Radiation-Induced Lung Toxicity in Patients With Non-Small-Cell Lung Cancer: A Combined Analysis From Beijing and Michigan

Zhao, Lujun; Wang, Lühua; Ji, Wei; Wang, Xiaozhen; Zhu, Xiaozhong; Hayman, James A.; Kalemkerian, Gregory P.; Yang, Wei; Brenner, Dean E.; Lawrence, Theodore S.; Kong, Feng-Ming

doi:10.1016/j.ijrobp.2008.10.065

Cited by 89 publications

(60 citation statements)

References 35 publications

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“…For example, among studies on the ability of TGF-β1 levels to predict radiation lung toxicity (4,5,8,23,24), two reporting negative results used insufficient gravitational force during the plasma preparation (8,24). These results validated our previous finding from one single Institute that blood sample handing process has significantly impact on the measure level of TGF-β1 in blood (11).…”

Section: Discussionsupporting

confidence: 65%

Ensuring sample quality for blood biomarker studies in clinical trials: a multicenter international study for plasma and serum sample preparation

Kong

Zhao

Wang

et al. 2017

Transl. Lung Cancer Res.

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Background: Sample quality is critical for biomarker detection in oncology, and platelet degradation and contamination in plasma have a remarkable impact on the ability to accurately quantify many blood-based biomarkers. Platelet factor 4 (PF4) can be used as an indicator to monitor sample quality. This multicenter study aimed to determine the impact of critical components of the blood sample handling process on platelet degradation/contamination and to establish an optimal method for collecting platelet-poor plasma samples.Methods: At each of six participating centers, blood samples were drawn from 12-13 healthy volunteers.Serum and plasma samples were prepared from whole blood samples using nine different methods that have been commonly used in ongoing multicenter trials. PF4 levels in the prepared samples were measured by enzyme-linked immunosorbent assay (ELISA). Paired t-tests were used for statistical analysis.Results: Blood samples were collected from 74 subjects enrolled in six centers. PF4 levels were significantly higher in serum samples than in plasma samples (P<0.001), in plasma samples from blood that sat at room temperature for 5 minutes (P=0.021), in plasma samples prepared at an insufficient centrifugal force (P<0.001), and in plasma samples prepared from blood that sat for longer than 4 hours on ice (P=0.001). For each method, the PF4 levels did not differ significantly among the centers or between Chinese and American subjects. The methods that resulted in normal levels of PF4 involved keeping blood samples on ice for 30 minutes to <4 hours and centrifugation at 2,500-3,000 ×g for 30 min.Conclusions: This multicenter study evaluated multiple blood sample handling conditions for minimizing platelet degradation during plasma serum preparation and determined an optimal method for preparing platelet-poor plasma. The findings of this study can be applied in future blood biomarker studies.

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Section: Discussionsupporting

confidence: 65%

Ensuring sample quality for blood biomarker studies in clinical trials: a multicenter international study for plasma and serum sample preparation

Kong

Zhao

Wang

et al. 2017

Transl. Lung Cancer Res.

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“…Though, the functional relevance of lipid droplet formation for radiation-induced pneumopathy remains to be defined. We speculate that as biomarkers for radiation-induced pneumopathy in patients [18,19]. Moreover, a time-dependent increase in tissue levels of TGF-ß, tumor necrosis factor-α, IL-1ß, and IL-6 mRNA as well as of MCP-1 and 3 and MIP-1γ proteins levels has been described in irradiated mice [13,17,20].…”

Section: Discussionmentioning

confidence: 78%

New insights into the molecular pathology of radiation-induced pneumopathy

Cappuccini¹,

Eldh

Bruder

et al. 2011

Radiotherapy and Oncology

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“…At the cellular level, RP is characterized by the infiltration of inflammatory cells into the pulmonary interstitium and alveolar spaces. Currently, both animal data and clinical reports have shown changes in the production of cytokines during radiotherapy and have suggested that these biologic factors could help to identify patients at risk of RP (16,17).…”

Section: Introductionmentioning

confidence: 99%