Elevation of Pectobacterium carotovorum subsp. odoriferum to species level as Pectobacterium odoriferum sp. nov., proposal of Pectobacterium brasiliense sp. nov. and Pectobacterium actinidiae sp. nov., emended description of Pectobacterium carotovorum and description of Pectobacterium versatile sp. nov., isolated from streams and symptoms on diverse plants

Portier, Pérrine; Pédron, Jacques; Taghouti, Géraldine; Saux, Marion Fischer-Le; Caullireau, Emma; Bertrand, Claire; Laurent, Angélique; Chawki, Khaoula; Oulgazi, Saïd; Moumni, Mohedine; Andrivon, Didier; Dutrieux, Cécile; Faure, Denis; Hélias, Valerie; Barny, Marie‐Anne

doi:10.1099/ijsem.0.003611

Cited by 150 publications

(130 citation statements)

References 31 publications

Supporting

Mentioning

117

Contrasting

Unclassified

Order By: Relevance

“…Later, a number of further separations of P. carotovorum subsp. carotovorum were offered, forming new species, polaris (Dees et al, 2017), maceratum/versatile (Shirshikov et al, 2018;Portier et al, 2019), peruviense (Waleron et al, 2018, and aquaticum , based on genomic analysis of available MLST markers, draft and complete genomes in databases. Recently, a substantial redistribution of pectobacterial taxonomy was processed, elevating a number of P. carotovorum subspecies to the species level, including Pbr .…”

Section: Properties and Genomics Of Strain F152 (Pb29)mentioning

confidence: 99%

“…Recently, a substantial redistribution of pectobacterial taxonomy was processed, elevating a number of P. carotovorum subspecies to the species level, including Pbr . Strain F152 follows the general biochemical and physiological properties typical for Pbr species (Nabhan et al, 2012;Portier et al, 2019). Bacterial cells of strain F152 are gram-negative, facultative anaerobes, negative for oxidase, urease, indol production, and gelatin liquefaction.…”

Section: Properties and Genomics Of Strain F152 (Pb29)mentioning

confidence: 99%

See 1 more Smart Citation

Morphologically Different Pectobacterium brasiliense Bacteriophages PP99 and PP101: Deacetylation of O-Polysaccharide by the Tail Spike Protein of Phage PP99 Accompanies the Infection

et al. 2020

View full text Add to dashboard Cite

Soft rot caused by numerous species of Pectobacterium and Dickeya is a serious threat to the world production of potatoes. The application of bacteriophages to combat bacterial infections in medicine, agriculture, and the food industry requires the selection of comprehensively studied lytic phages and the knowledge of their infection mechanism for more rational composition of therapeutic cocktails. We present the study of two bacteriophages, infective for the Pectobacterium brasiliense strain F152. Podoviridae PP99 is a representative of the genus Zindervirus, and Myoviridae PP101 belongs to the still unclassified genomic group. The structure of O-polysaccharide of F152 was established by sugar analysis and 1D and 2D NMR spectroscopy:The recombinant tail spike protein of phage PP99, gp55, was shown to deacetylate the side chain talose residue of bacterial O-polysaccharide, thus providing the selective attachment of the phage to the cell surface. Both phages demonstrate lytic behavior, thus being prospective for therapeutic purposes.

show abstract

Section: Properties and Genomics Of Strain F152 (Pb29)mentioning

confidence: 99%

Section: Properties and Genomics Of Strain F152 (Pb29)mentioning

confidence: 99%

Morphologically Different Pectobacterium brasiliense Bacteriophages PP99 and PP101: Deacetylation of O-Polysaccharide by the Tail Spike Protein of Phage PP99 Accompanies the Infection

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Expression levels of the selected genes from different functional categories were verified by qPCR and found to correlate well with RNA-seq data (data not shown). 2 Only the first gene(s) of the larger operon shows differential expression 3 Divergently transcribed operons sharing the same regulatory region are shown as a single divergon. The largest of the fold change values is shown 4 Gene(s) are silent in the phoP mutant but expressed in the wild type cells…”

Section: Phopq System Controls Over a Hundred Genes In P Versatilementioning

confidence: 99%

“…Recently, more isolates from various sources, including Chrysanthemum, Iris and water, were united with Ca. P. maceratum strains and renamed as Pectobacterium versatile (2). The P. versatile taxon includes two strains which have been well characterised experimentally, Ecc71 and SCC1.…”

Section: Introductionmentioning

confidence: 99%

The PhoPQ two-component system is the major regulator of cell surface properties, stress responses and plant-derived substrate utilisation during development ofPectobacterium versatile-host plant pathosystem

Kravchenko

Gogoleva

Kolubako

et al. 2020

Preprint

View full text Add to dashboard Cite

The PhoPQ two-component system, originally described in pectobacteria as PehRS, was previously shown to regulate a single gene, pehA. Using an insertional phoP mutant of Pectobacterium versatile, we demonstrate that PhoP controls a regulon of at least 116 genes with a large fraction of regulon members specific for pectobacteria. The functions performed by the PhoP controlled genes include transport and metabolism of plant-derived carbon sources (polygalacturonate, arabinose and citrate), modification of bacterial cell envelope and stress resistance. High concentrations of calcium and magnesium ions were found to abolish the PhoPQdependent transcription activation. Reduced PhoP expression and minimisation of PhoP dependence of regulon members' expression in the cells isolated from rotten potato tuber tissues suggest that PhoPQ system may adjust expression levels of multiple virulence-related genes during the course of P. versatile-host plant pathosystem development.PehR was originally described as a transcriptional activator of the pehA gene encoding endopolygalacturonase, a major virulence factor in soft rot bacteria (16). PehR is a response regulator that forms a two-component sensory system (TCS) with the membrane histidine kinase PehS. In the SCC3193 strain, which is currently classified as Pectobacterium parmentieri, inactivation of either pehR or pehS resulted in reduced virulence and Ca 2+ was reported as the ligand detected by the PehS sensor (16,17). It was also suggested that the PehRS system is responsible for the decrease of the polygalacturonase and increase of the pectate lyase activities in response to Ca 2+ released from the degraded cell walls (17).The pectobacterial PehRS two-component system received very little attention since its discovery two decades ago. However, much information has been accumulated about the orthologous system called PhoPQ in other bacteria from the order Enterobacetrales. In Dickeya dadantii, which belongs to the Pectobacteriaceae family together with Pve, PhoPQ TCS was shown to control pellicle formation, motility, resistance to cationic antimicrobial peptides and expression of pectate lyases in response to Mg 2+ concentration (18,19). The PhoPQ system has been thoroughly characterised in Salmonella and to a lesser extent in few more Enterobacteriaceae species including Escherichia coli and Yersinia spp. (see (20) for a review). The PhoQ sensor histidine kinase activates PhoP by phosphorylation in response to several stimuli, including low Mg 2+ concentration (21), cationic peptides (22), acidity (23) and high osmolarity (24). PhoP binds to direct repeats with a consensus gGTTTA which seems to be well conserved in enterobacteria (25,26). The regulon composition nevertheless varies widely even between closely related bacteria. In Salmonella enterica, over a hundred genes are regulated by PhoP, but only three of them (phoP, phoQ and slyB) are always under PhoP control in other enterobacteria. Some regulon members are shared by several species, but most PhoP controlled gene...

show abstract

“…odoriferum NCPPB3841 (isolated from Cichorium intybus in France, 1979) and P. polaris NCPPB3395 (Dees et al, 2017) were obtained from the National Collection of Plant Pathogenic Bacteria (NCPPB), United Kingdom. In addition, P. versatile SCC1 (CFBP8537) (Niemi et al, 2017;Portier et al, 2019) and P. parmentieri SCC3193 (CFBP8536) (Nykyri et al, 2012;Khayi et al, 2016) stored at the University of Helsinki were used. The strains were grown overnight at 28 °C in liquid Luria-Bertani (LB) medium (1% tryptone, 0.5% yeast extract, 0.5% NaCl) with shaking (200 rpm) or on LB plates solidified with 1.5% agar unless otherwise stated.…”

Section: Bacterial Strainsmentioning

confidence: 99%

Single gene enables plant pathogenic Pectobacterium to overcome host‐specific chemical defence

Bosch

Niemi

Welte

2019

Molecular Plant Pathology

View full text Add to dashboard Cite

Plants of the Brassicales order, including Arabidopsis and many common vegetables, produce toxic isothiocyanates to defend themselves against pathogens. Despite this defence, plant pathogenic microorganisms like Pectobacterium cause large yield losses in fields and during storage of crops. The bacterial gene saxA was previously found to encode isothiocyanate hydrolase that degrades isothiocyanates in vitro. Here we demonstrate in planta that saxA is a virulence factor that can overcome the chemical defence system of Brassicales plants. Analysis of the distribution of saxA genes in Pectobacterium suggests that saxA from three different phylogenetic origins are present within this genus. Deletion of saxA genes representing two of the most common classes from P. odoriferum and P. versatile resulted in significantly reduced virulence on Arabidopsis thaliana and Brassica oleracea. Furthermore, expressing saxA from a plasmid in a potato‐specific P. parmentieri strain that does not naturally harbour this gene significantly increased the ability of the strain to macerate Arabidopsis. These findings suggest that a single gene may have a significant role in defining the host range of a plant pathogen.

show abstract

Cited by 150 publications

References 31 publications

Morphologically Different Pectobacterium brasiliense Bacteriophages PP99 and PP101: Deacetylation of O-Polysaccharide by the Tail Spike Protein of Phage PP99 Accompanies the Infection

Morphologically Different Pectobacterium brasiliense Bacteriophages PP99 and PP101: Deacetylation of O-Polysaccharide by the Tail Spike Protein of Phage PP99 Accompanies the Infection

The PhoPQ two-component system is the major regulator of cell surface properties, stress responses and plant-derived substrate utilisation during development ofPectobacterium versatile-host plant pathosystem

Single gene enables plant pathogenic Pectobacterium to overcome host‐specific chemical defence

Contact Info

Product

Resources

About