Elevation of neuron-specific enolase and S-100β protein level in experimental acute spinal cord injury

Cao, Fei; Yang, Xiaofeng; Liu, Weiguo; Hu, Weiwei; Gu, Li; Zheng, Xianliang; Shen, Fang; Zhao, Xue-qun; Lv, Shi-ting

doi:10.1016/j.jocn.2007.05.014

Cited by 63 publications

(47 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10,27 This has also been identified in patients at risk of an ischemic SCI during endovascular stent grafting where S-100b concentrations peak at 6 h post injury. 10 GFAP on the other hand has been reported to reach peak levels after 24 h in ischemic SCI.…”

Section: Discussionmentioning

confidence: 96%

Structural biomarkers in the cerebrospinal fluid within 24 h after a traumatic spinal cord injury: a descriptive analysis of 16 subjects

et al. 2014

View full text Add to dashboard Cite

Study design: Prospective cohort study. Objectives: To characterize the cerebrospinal fluid (CSF) concentrations of glial fibrillary acidic protein, neuron specific enolase (NSE), S-100b, tau and neurofilament heavy chain (NFH) within 24 h of an acute traumatic spinal cord injury (SCI), and to correlate these concentrations with the baseline severity of neurologic impairment as graded by the American Spinal Injury Association impairment scale (AIS). Methods: A lumbar puncture was performed to obtain CSF from 16 acute traumatic SCI patients within 24 h post injury. Neurological examinations were performed within 24 h of injury and again at 6 or 12 months post injury. The correlations between the CSF concentrations and initial AIS were calculated by using Pearson correlation coefficients. In addition, an independent Student's t-test was used to test for differences in CSF concentrations between patients of different AIS grades. Results: The CSF NSE concentrations were significantly correlated with the baseline neurologic impairment being either 'motor complete' (AIS A, B) or 'motor incomplete' (AIS C, D) (r ¼ 0.520, Po0.05). The mean S-100b concentration in motor complete patients was significantly higher compared with motor incomplete patients; 377.2 mg l À1 (s.d. ± 523 mg l À1 ) vs 57.1 mg l À1 (s.d.±56 mg l À1 ) (Po0.05), respectively. Lastly, the mean NFH concentration in motor complete patients was significantly higher compared with motor incomplete patient, 11 813 ng l À1 (s.d.±16 195 ng l À1 ) vs 1446.8 ngl À1 (s.d.±1533 ng l À1 ), (Po0.05), respectively. Conclusion: In this study we identified differences in the structural CSF biomarkers NSE, S-100b and NFH between motor complete and motor incomplete SCI patients. Our data showed no clear differences in any of the protein concentrations between the different AIS grades.

show abstract

Section: Discussionmentioning

confidence: 96%

Structural biomarkers in the cerebrospinal fluid within 24 h after a traumatic spinal cord injury: a descriptive analysis of 16 subjects

et al. 2014

View full text Add to dashboard Cite

show abstract

“…This 373 enzyme is a marker of ischemic brain damage (74) and although it only has a short biologic half-life 374 (≤ 24h) (75), NSE holds promise as an acute indicator of neuronal damage. NSE levels are elevated in the CSF, plasma (76) and serum (77) of rats in the acute phase of SCI.…”

mentioning

confidence: 93%

“…S100 is involved in a diverse range of functions including calcium homeostasis, 396 enzyme activity and metabolism, cell proliferation and differentiation (80). Measurement of S100β 397 has potential as an acute marker of SCI, as it is significantly increased in the blood (76,77,81) and 398 CSF (76) of rats at 6h after severe contusion injury compared to sham injury. In the human acute 399 setting (<48h), S100β is also increased in the serum of patients with vertebral spine fractures (mean=0.77 μg/L; n=34) compared to uninjured patients (0.14 μg/L; n=29) (78) …”

mentioning

confidence: 99%

The developing landscape of diagnostic and prognostic biomarkers for spinal cord injury in cerebrospinal fluid and blood

Fuller

et al. 2016

Spinal Cord

View full text Add to dashboard Cite

show abstract

“…In the literature, the expression of neuron-specific enolase (g-enolase) is increased with prolonged time of acute SCI. 13 However, enolase was downregulated in our study, possibly representing the pathological differences between acute and ischemia-reperfusion SCI. PKM2, SDHA, PYGM, PYGB and GPD2 are carbon-centric enzymes involved in glycolysis and the tricarboxylic acid cycle.…”

Section: Protein Validation By Western Blot and Immunohistochemistrymentioning

confidence: 49%

Dynamic proteome analysis of spinal cord injury after ischemia–reperfusion in rabbits by two-dimensional difference gel electrophoresis

Zhu

et al. 2013

Spinal Cord

View full text Add to dashboard Cite

Study design: Spinal cord injury (SCI) is a devastating and common neurologic disorder that has profound influences on modern society from physical, psychosocial and socio-economic perspectives. Objectives: To analyze the dynamic changes in protein expression during SCI after ischemia-reperfusion. Methods: We used two-dimensional difference gel electrophoresis combined with matrix-assisted laser desorption/ionization time-offlight/time-of-flight MS to give a global analysis of protein dynamic change during SCI after ischemia-reperfusion. Dynamic changes in protein expression were investigated from 6 to 48 h in SCI after ischemia-reperfusion using a proteomics tool. Results: Twenty-one proteins were identified in total, including neuronal proteins, glycometabolism enzymes, stress-related proteins and cytoskeleton-related proteins. These were divided into upregulated and downregulated groups. Results identified 24 h as a key time point when all proteins were changed dramatically. In addition, changes in Fascin expression were discovered in SCI for the first time. Conclusion: In conclusion, we observed dynamic proteome change correlated with SCI by ischemia-reperfusion, and provided a clue to this pathological mechanism by protein identification and analysis.

show abstract

Elevation of neuron-specific enolase and S-100β protein level in experimental acute spinal cord injury

Cited by 63 publications

References 10 publications

Structural biomarkers in the cerebrospinal fluid within 24 h after a traumatic spinal cord injury: a descriptive analysis of 16 subjects

Structural biomarkers in the cerebrospinal fluid within 24 h after a traumatic spinal cord injury: a descriptive analysis of 16 subjects

The developing landscape of diagnostic and prognostic biomarkers for spinal cord injury in cerebrospinal fluid and blood

Dynamic proteome analysis of spinal cord injury after ischemia–reperfusion in rabbits by two-dimensional difference gel electrophoresis

Contact Info

Product

Resources

About