Elevation of Blood Pressure During Pregnancy in Uninephrectomized and Salt-Loaded Wistar Rats

Nagai, Yohko; Yamaguchi, Shuji; Fujikawa, Katsuyuki; Nishihara, Masahiro; Nakanishi, Kazushige; Nakamura, Katsuhiko

doi:10.3109/10641959609015689

Cited by 1 publication

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[7][8][9] In 1996, we reported that uninephrectomized and saltloaded pregnant Wistar rats showed a preeclampsia-like syndrome characterized by an increase in blood pressure with mild proteinuria during pregnancy. 10 We histologically examined the glomeruli, but found no morphological changes except for glomerular hypertrophy. At present, generalized endothelial disorders of the vasculature are considered to be the most important factor in the pathophysiology of preeclampsia/eclampsia.…”

Section: Introductionmentioning

confidence: 99%

Preeclampsia-like glomerular lesions induced by a single injection of lipopolysaccharide (LPS) in pregnant rats

Nagai

Nakanishi

et al. 2002

Clinical and Experimental Nephrology

View full text Add to dashboard Cite

Background. We induced pathological changes in the glomerular capillary walls by causing damage to endothelial cells produced by lipopolysaccharide (LPS) injection in pregnant rats and compared the induced lesions with those of human preeclamptic nephropathy, known as glomerular endothelial damage. Methods. Forty-three uninephrectomized and salt-loaded Wistar rats were divided into three groups. Twenty-six pregnant rats (Group I) and 9 nonpregnant rats (Group II) were injected intravenously with LPS (E. coli 055:B5, 130 µg/kg) on day 15 of gestation, and 8 pregnant rats (Group III) were injected with vehicle. The kidneys of the rats were pathologically and physiologically examined on day 20. Glomerular hemodynamic changes were evaluated under a real-time confocal laser-scanning microscope (CLSM) with a high-speed CCD video camera. Results. Twenty-one (81%) rats in Group I showed glomerular microangiopathic lesions detected as endothelial swelling, a double contour of the basement membrane, and intracapillary fibrin deposits by light and electron microscopy. Immunofluorescent analysis showed that fibrinogen deposition colocalized with the endothelial cell markers. The lesions were observed in only one rat (11%) in Group II. The glomerular blood flow decreased significantly in Group I compared with Group III (P Ͻ 0.05, 218.7 Ϯ 122.0 vs 883.2 Ϯ 122.0 µm/s).Conclusion. The glomerular lesions induced by injecting LPS in pregnant rats showed endothelial cell abnormalities and secondary intracapillary coagulation. We consider that these glomerular lesions were "specified" during gestation, and they had many histopathological and physiological findings in common with human preeclamptic nephropathy.

show abstract

Section: Introductionmentioning

confidence: 99%