Elevation of antibodies to xanthine oxidase in myocardial infarction

Harrison, R. J.; Benboubetra, Mustapha; Bryson, Shona; Elwood, Peter Creighton; Thomas, R.D.

doi:10.1016/0022-2828(90)91684-y

Cited by 2 publications

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“…Indeed, levels of both XO (55) and anti-XO antibodies (56) have been shown to be elevated in plasma of atherosclerosis patients, and the enzyme has been detected in their vessel walls and plaques (57,58).…”

Section: Discussionmentioning

confidence: 99%

Reduction of Nitrite to Nitric Oxide Catalyzed by Xanthine Oxidoreductase

Godber¹,

Doel²,

Sapkota³

et al. 2000

Journal of Biological Chemistry

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370

288

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Xanthine oxidase (XO) was shown to catalyze the reduction of nitrite to nitric oxide (NO), under anaerobic conditions, in the presence of either NADH or xanthine as reducing substrate. NO production was directly demonstrated by ozone chemiluminescence and showed stoichiometry of approximately 2:1 versus NADH depletion. With xanthine as reducing substrate, the kinetics of NO production were complicated by enzyme inactivation, resulting from NO-induced conversion of XO to its relatively inactive desulfo-form. Steady-state kinetic parameters were determined spectrophotometrically for urate production and NADH oxidation catalyzed by XO and xanthine dehydrogenase in the presence of nitrite under anaerobic conditions. pH optima for anaerobic NO production catalyzed by XO in the presence of nitrite were 7.0 for NADH and <6.0 for xanthine. Involvement of the molybdenum site of XO in nitrite reduction was shown by the fact that alloxanthine inhibits xanthine oxidation competitively with nitrite. Strong preference for Mo‫؍‬S over Mo‫؍‬O was shown by the relatively very low NADH-nitrite reductase activity shown by desulfo-enzyme. The FAD site of XO was shown not to influence nitrite reduction in the presence of xanthine, although it was clearly involved when NADH was the reducing substrate. Apparent production of NO decreased with increasing oxygen tensions, consistent with reaction of NO with XO-generated superoxide. It is proposed that XO-derived NO fulfills a bactericidal role in the digestive tract.

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Section: Discussionmentioning

confidence: 99%

Reduction of Nitrite to Nitric Oxide Catalyzed by Xanthine Oxidoreductase

Godber¹,

Doel²,

Sapkota³

et al. 2000

Journal of Biological Chemistry

Self Cite

370

288

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“…Peroxynitrite, from whatever source, has the capacity to effect endothelial injury [38] and may be seen as particularly relevant to cardiovascular disease. Indeed, levels of both XOR [39] and anti‐XOR antibodies [40] are known to be elevated in plasma of atherosclerosis patients and the presence of the enzyme has been demonstrated in their vessel walls and plaques [41,42].…”

Section: Discussionmentioning

confidence: 99%

A new route to peroxynitrite: a role for xanthine oxidoreductase

Godber¹,

Doel²,

Durgan³

et al. 2000

FEBS Letters

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Peroxynitrite, a potent oxidising, nitrating and hydroxylating agent, results from the reaction of nitric oxide with superoxide. We show that peroxynitrite can be produced by the action of a single enzyme, xanthine oxidoreductase (XOR), in the presence of inorganic nitrite, molecular oxygen and a reducing agent, such as pterin. The effects of oxygen concentration on peroxynitrite production have been examined. The physiologically predominant dehydrogenase form of the enzyme is more effective than the oxidase form under aerobic conditions. It is proposed that XOR-derived peroxynitrite fulfils a bactericidal role in milk and in the digestive tract. ß

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Elevation of antibodies to xanthine oxidase in myocardial infarction

Cited by 2 publications

References 0 publications

Reduction of Nitrite to Nitric Oxide Catalyzed by Xanthine Oxidoreductase

Reduction of Nitrite to Nitric Oxide Catalyzed by Xanthine Oxidoreductase

A new route to peroxynitrite: a role for xanthine oxidoreductase

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