Elevation in metallothionein messenger RNA in rat tissues after exposure to X-irradiation

Shiraishi, Noriyuki; Hayashi, Hidehiro; Hiraki, Yoshio; Aono, Kaname; Itano, Yoshitaro; Kosaka, Futami; Noji, Sumihare; Taniguchi, Shigehiko

doi:10.1016/0041-008x(89)90178-6

Cited by 37 publications

(5 citation statements)

References 15 publications

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“…[18"19] MT is characterized by a high thiol content and absence of aromatic amino acids, including tyrosine, tryptophan, and phenylalanine. [21 The synthesis of MT is increased by various stressful conditions causing oxidative stress including high oxygen tension, I4°l X-ray irradiation, [41][42][43] UV irradiation, [44] The results outlined in this report provide the direct evidence using ESR that MT-I and II are able to protect against diphenyl picrylhydrazyl radicals (Figure 3 Although both MT-I and II were efficacious in scavenging free radicals, the reason for a greater potency of MT-I is not clear at this time. Twenty of the 61 amino acid residues in the molecule are cysteinyl residues, all of which are involved in metal binding.…”

Section: Discussionmentioning

confidence: 99%

Free radical scavenging actions of metallothionein isoforms I and II

Kumari

Hiramatsu²,

Ebadi

1998

Free Radical Research

220

123

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By employing electron spin resonance spectroscopy, we examined the free radicals scavenging effects of hepatic metallothionein (MT) isoforms I and II (MTs-I and II) on four types of free radicals. Solutions of 0.15 mM of MT-I and 0.3 mM of MT-II were found to scavenge the 1,1-diphenyl-2-picrylhydrazyl radicals (1.30 x 10(15) spins/ml) completely. In addition, both isoforms exhibited total scavenging action against the hydroxyl radicals (1.75 x 10(15) spins/ml) generated in a Fenton reaction. Similarly, 0.3 mM of MT-I scavenged almost 90% of the superoxide (2.22 x 10(15) spins/ml) generated by the hypoxanthine and xanthine oxidase system, while a 0.3 mM MT-II solution could only scavenge 40% of it. By using 2,2,6,6-tetramethyl-4-piperidone as a "spin-trap" for the reactive oxygen species (containing singlet oxygen, superoxide and hydroxyl radicals) generated by photosensitized oxidation of riboflavin and measuring the relative signal intensities of the resulting stable nitroxide adduct, 2,2,6,6-tetramethyl-4-piperidine-1-oxyl, we observed that MT-II (0.3 mM) could scavenge 92%, while MT-I at 0.15 mM microl/ml concentrations could completely scavenge all the reactive species (2.15 x 10(15) spins/ml) generated. The results of these studies suggest that although both isoforms of MT are able to scavenge free radicals, the MT-I appears to be a superior scavenger of superoxide and 1,1 diphenyl-2-picrylhydrazyl radicals.

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Section: Discussionmentioning

confidence: 99%

Free radical scavenging actions of metallothionein isoforms I and II

Kumari

Hiramatsu²,

Ebadi

1998

Free Radical Research

220

123

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“… 1 MT is constitutively expressed in various species and tissues, including testis, and is considered as one of the stress-response proteins. 2 In the liver, MT protein is induced readily by many stress-associated challenges such as glucocorticoids, bacterial lipopolysaccharide, interferon, alkylating agents and irradiation. 3 These challenges produce reactive oxygen species (ROS) or radicals, which damage tissues and cells, and elicit acute or chronic inflammation.…”

Section: Introductionmentioning

confidence: 99%

Diabetes Mellitus induces alterations in metallothionein protein expression and metal levels in the testis and liver

Lian

Sun

et al. 2017

J Int Med Res

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ObjectiveTo investigate the effect of diabetes with and without vitamin E treatment on testicular metallothionein (MT) and metal (zinc, copper and iron) changes.MethodsDiabetes was induced with a single intraperitoneal injection (i.p.) of streptozotocin in rats, and diabetic rats were given Vitamin E by i.p. every other day for 4 weeks. MT protein was measured by the cadmium-haeme assay and metal levels were detected by an atomic absorption spectrophotometer.ResultsDiabetes did not change testicular MT protein, but significantly increased hepatic MT protein. Diabetes significantly decreased testicular copper, but not hepatic copper. Zinc and iron levels were unchanged in both diabetic testis and liver. Vitamin E significantly enhanced both testicular and hepatic MT, and zinc levels in diabetic rats. Vitamin E slightly decreased the copper levels, but did not change the testicular and hepatic iron in diabetic rats.ConclusionsTesticular MT protein expression was not increased, even though hepatic MT significantly increased independent of metal changes, in diabetic rats. Vitamin E enhanced testicular and hepatic MT, which correlated with increased zinc levels.

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“…Pooled tissues re-NEN). Hybridization and posthybridization washes were carried out at 42 °C and 68 °C, respectively, as described previously [27], The damp filter was wrapped in Saran Wrap, and exposed to X-ray film (X-Omat AR, Eastman Kodak) using an intensifying screen at -8 0 °C. The autoradiograms were analyzed moved from 5-10 mice were used for the metal deter mination.…”

Section: Methodsmentioning

confidence: 99%

Metallothionein Messenger RNA Levels in the Macular Mutant Mouse: An Animal Model of Menkes’ Kinky-Hair Disease

Shiraishi

Taguchi

Kinebuchi³

1991

Neonatology

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Levels of metallothionein-1 (MT-1) messenger RNA (mRNA) in various tissues from normal and macular mutant mice at different stages of development (17 days of gestation, 1-, 3-, 7- and 14-day-old) were determined by Northern blot analysis. Renal MT-1 mRNA levels in mutant mice were slightly elevated at 3 stages compared to normal mice, with the exception of mutant fetus and 3-day-old mutant mice. Intestinal MT-1 mRNA levels in mutant mice were elevated at 3 stages compared to normal mice with the exception of mutant fetus and 3-day-old mutant mice. Hepatic MT-1 mRNA levels in mutant fetus and 1-day-old mutant mice were approximately the same compared to normal mice. In 3- and 7-day-old mutant mice, hepatic MT-1 mRNA levels were depressed and in 14-day-old mutant mice, they were increased compared to normal mice. Brain MT-1 mRNA could not be detected in normal and mutant mice at the 4 stages with the exception of 14-day-old mice. MT-1 mRNA in 14-day-old mice was detected and the level of that in mutant mice was slightly elevated compared to normal mice. After injection of Cu, MT-1 mRNA levels in kidney, liver and intestine were determined. The injection of Cu increased the level of MT-1 mRNA in the tissues of normal and mutant mice compared to control (saline-injected) mice. Significant differences in MT-1 mRNA levels in the tissues of both Cu-injected mice were not observed.

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Elevation in metallothionein messenger RNA in rat tissues after exposure to X-irradiation

Cited by 37 publications

References 15 publications

Free radical scavenging actions of metallothionein isoforms I and II

Free radical scavenging actions of metallothionein isoforms I and II

Diabetes Mellitus induces alterations in metallothionein protein expression and metal levels in the testis and liver

Metallothionein Messenger RNA Levels in the Macular Mutant Mouse: An Animal Model of Menkes’ Kinky-Hair Disease

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