Elevated tissue levels of collagenase during dilation of uterine cervix in human parturition

Rajabi, Mohammad Taher; Dean, David D.; Beydoun, Samir N.; Woessner, Jeff

doi:10.1016/s0002-9378(88)80183-2

Cited by 141 publications

(45 citation statements)

References 13 publications

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“…Upon the death of cervical cells and subsequent infiltration of monocytes, proteolytic enzymes are re leased from these cells [24][25][26][27] that further degrade inter cellular materials including myosin and actin. These pro teases which modify the specific protease inhibitors may initiate the hydrolysis of connective tissues and lead to the softening of the cervix [24][25][26][27],…”

Section: Discussionmentioning

confidence: 99%

“…This process of apoptosis in ripening cervices resembles the deletion of cell popula tions that occurs in normal development and metamor phosis [28,29]. Furthermore, apoptosis is regulated by hormones [10,11,13,14], paracrines and cytokines [24][25][26][27], Interestingly, the onset of labor and cervical ripening has been shown to be regulated by estradiol and progester one antagonists [30][31][32][33], adrenocorticotropin [34] and prostaglandins [35].…”

Section: Discussionmentioning

confidence: 99%

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Apoptosis in the Cervix of Pregnant Rats in Association with Cervical Softening

Leppert

Yu²

1994

Gynecol Obstet Invest

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Cervical ripening in gestation occurs spontaneously in a timely but species specific fashion as if it were genetically programmed. This study shows that the numbers of dying smooth muscle cells in the cervix increased along with cervical softening in pregnant rats from day 12 of gestation to day 21. The morphological characteristics of the chromatin cleavage in apoptosis are identified. Internucleosomal DNA fragments in the DNA preparations as well as the ladder pattern of double-stranded DNA observed on gel electrophoresis are observed. The deletion and decrease of the cell population in the cervices was demonstrated by a decrease of total DNA in these tissues. The interesting possibility is raised that programmed smooth muscle cell death in the cervix may be involved in the processes of the cervical ripening.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Apoptosis in the Cervix of Pregnant Rats in Association with Cervical Softening

Leppert

Yu²

1994

Gynecol Obstet Invest

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“…Serine protease inhibitors and tissue inhibitors of metalloproteinases are groups of enzyme inhibitors that bind with enzymes and, in this case, regulate serine collagenase and metallocollagenase activities (Woessner, 1991;Salzet et al, 1999). Disrupting these bonds can be an important mechanism in activating latent collagenases (Rajabi et al, 1988). Metallocollagenases are, also, inhibited by serum proteins such as α 2 -macroglobulin (Sakamoto et al, 1972;Nagai, 1973;Sellers et al, 1977;Shinkai et al, 1977;Woessner, 1991).…”

Section: Factors Affecting Collagenase Activitymentioning

confidence: 99%

“…DTT, iodoacetamide and potassium iodide seem to follow a similar mechanism involving the removal, degradation or denaturation of the inhibitor (Abe and Nagai, 1973;Shinkai et al, 1977;Rajabi et al, 1988). Reagents such as DTT require care when used as activators because their thiolreducing nature that results in degradation of the inhibitor is, also, capable of inhibiting collagenase.…”

Section: Factors Affecting Collagenase Activitymentioning

confidence: 99%

Extraction and Purification of Collagenase Enzymes: A Critical Review

Daboor¹,

Budge²,

Ghaly

et al. 2010

American Journal of Biochemistry and Biotechnology

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Problem statement: Enzymes have vital roles in several industrial processes (foods, cosmetics, nutraceuticals and pharmaceuticals) due to their highly selective nature and high activity at very low concentrations. Recent efforts to identify new sources of useful enzymes have been concentrated on the marine environment because of the potential to make use of processing wastes. About 35-50% of the mass of the fish caught is a waste that is disposed off at sea or in landfills. The extraction of enzymes from fish processing waste can reduce environment problems and improve the economics of the fish industry. Collagenases are a group of enzymes that can be extracted from fish waste. Approach: Comprehensive reviews of the literature on the extraction, purification, characterization and use of collagenases was carried out. Results: Collagenases have different molecular weights based on their types and sources. They have the ability to break down the peptide bonds in collagen at physiological pH. They are classified into two types: serine and metallocollagenase. Collagenolytic activities have been shown at a wide range of temperatures (20-40°C) and pH (6-8). Many activators can be used to achive collagenase activity including 4-Aminophenylmercuric Acetate (APMA), trypsin, potassium or sodium thiocyanate, iodoacetamide and potassium iodide. Dithiothreitol (DTT), mercaptoethanol, ethylendiaminetetracetic acid, ophenanthroline and cysteine inactivate the enzyme. Collagenases enzymes can be extracted with a variety of techniques using different buffering systems (tris-HCl, sodium bicarbonate, calcium chloride and cacodylate). All techniques involve the use of ammonium sulphate fractionation and centrifugation to precipitate the enzyme. Collagenases are normally purified using chromatographic techniques such as gel-filtration, ion-exchange and affinity column chromatography. Collagenase can be assayed with a number of methods, including: colorimetric absorbance, viscometry, radioactivity and fluorescence spectroscopy. Collagenases are partly responsible for toughness in red meats and are used as tenderizers in food industry, have application in the fur and hide tanning to ensure uniform dying of leather, used in medicine to treat burns and ulcers, eliminate scar tissues, transplantation of organs. Conclusion: Understanding of the nature of the enzymes and identifying the most suitable resources and the methods for their extraction and purification will have significant impact on the fish processing, food and medical industries.

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“…and 0.72 for 7S collagen. These data suggest that serum 7S collagen closely reflects collagen degradation in the cer vix and so should be a good marker of cervical maturation.Cervical maturation is characterized by degradation of collagen through the actions of various collagenases [1,2], and this enzymatic breakdown of collagen and loss of col lagen fragments from the ripening cervical tissue result in cervical softening before labor [3,4], The main collagens of the cervix are types I, III and IV. There are several reports on changes of collagen types during pregnancy or labor [3], These changes have, however, mainly been mea sured in biopsy specimens, and little is known about changes in the blood levels of collagens.…”

mentioning

confidence: 99%

Relationship of Serum Levels of Pro-Type I Collagen Peptide, Pro-Type III Collagen Peptide and Type IV 7S Collagen with Cervical Maturation

Kanayama¹,

Terao²

1992

Gynecol Obstet Invest

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The serum levels of pro-type I collagen peptide (PIP), pro-type III collagen peptide (PIIIP) and type IV 7S collagen (7S collagen) were measured in 41 women in various gestational weeks of pregnancy (5–41 weeks). The PIP level did not change during pregnancy, being 450 ± 380 mg/ml before week 37 and 448 ± 280 mg/ml after week 37. The PIIIP level also did not change being 0.55 ± 0.21 and 0.59 ± 0.22 U/ml before and after 37 weeks. The PIIIP level increased slightly but not significantly after week 37. The 7S collagen level, however, increased significantly (p < 0.01), being 5.1 ± 0.8 and 6.6 ± 0.8 mg/ml before and after week 37. The correlation coefficients with the Bishop score were 0.22 for PIP, 0.42 for PIIIP, and 0.72 for 7S collagen. These data suggest that serum 7S collagen closely reflects collagen degradation in the cervix and so should be a good marker of cervical maturation.

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Elevated tissue levels of collagenase during dilation of uterine cervix in human parturition

Cited by 141 publications

References 13 publications

Apoptosis in the Cervix of Pregnant Rats in Association with Cervical Softening

Apoptosis in the Cervix of Pregnant Rats in Association with Cervical Softening

Extraction and Purification of Collagenase Enzymes: A Critical Review

Relationship of Serum Levels of Pro-Type I Collagen Peptide, Pro-Type III Collagen Peptide and Type IV 7S Collagen with Cervical Maturation

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