Abstract. Aluminium alloys are promising candidates for energy-and cost-efficient components in automotive and aerospace industries, due to their excellent strength-to-weight ratio and relatively low cost compared to titanium alloys. As modern cast processing and post-processing, e.g. hot isostatic pressing, result in decreased frequency and size of defects, the weakest link depends on microstructural characteristics, e.g. secondary dendrite arm spacing (SDAS), Si eutectic morphology and α-Al solid solution hardness. Hereby, fatigue investigations of the effect of the microstructure characteristics on the cyclic stress-strain behaviour as well as fatigue mechanisms in the low cycle and high cycle fatigue regime are performed. For this purpose, samples of the aluminium cast alloy EN AC-AlSi7Mg0.3 with different Si eutectic morphology and α-Al solid solution hardness were investigated. To compare the monotonic and cyclic stress-strain curves, quasistatic tensile tests and incremental step tests were performed on two microstructure conditions. The results show that the cyclic loading leads to a hardening of the material compared to monotonic loading. Based on damage parameter Woehler curves, it is possible to predict the damage progression and fatigue life for monotonic and cyclic loading in hypo-eutectic Al-Si-Mg cast alloys by one power law.