Elevated serum miR‐3129‐5p contributes to the progression of coronary heart disease via targeting mTOR

Abstract

The current study aims to explore the miRNA changes that occur in the serum of patients with coronary heart disease (CHD) and healthy controls using a microarray technique, thereby exploring the potential biomarkers in the diagnosis of CHD and the underlying mechanism. Clinical data were reviewed, and venous blood samples were collected from 66 cases of CHD and 58 cases of healthy controls. MicroRNAwide expression profiling identified 16 miRNAs that were aberrantly decreased bỹ 2-fold in the serum of patients with CHD compared to that of healthy controls. RT-PCR analysis indicated that the expression of miR-3129-5p was increased the most in patients with CHD compared with that of controls. Moreover, serum miR-3129-5p was found to be highest in the severe stenosis group, followed by the moderate stenosis group and mild stenosis group. ROC analysis showed that serum miR-3129-5p could differentiate patients with CHD from controls. Further study showed that mTOR was a target gene of miR-3129-5p. Western blot assays demonstrated that miR-3129-5p significantly suppressed the phosphorylation of S6 but increased LC3II/LC3I and Beclin1 levels. Consistently, GFP-LC3 and TEM assays indicated that miR-3129 increased autophagy puncta in H9C2 cells. More importantly, silencing mTOR significantly decreased the expression of p-S6 but increased LC3II/LC3I and Beclin expression even in H9C2 cells transfected with miR-3129-5p inhibitor, indicating that miR-3129-5p-induced cell autophagy was mediated via mTOR in H9C2 cells. In summary, elevated serum miR-3129-5p contributes to CHD by targeting mTOR signaling and may be a therapeutic target in the treatment of CHD.

Integrated analysis of miRNA-mRNA regulatory network and functional verification of miR-338-3p in coronary heart disease

“…De Yébenes et al con rmed that miRNA related to aging, miR-217, can aggravate atherosclerosis and promote cardiovascular dysfunction (de Yébenes et al, 2020). Wang et al also found that serum miR-3129-5p mediates mTOR to promote the progression of coronary heart disease, suggesting that miR-3129-5p may become one of the biological targets for the treatment of coronary heart disease (Wang et al, 2021). Therefore, we speculate that miR-338-3p may be involved in the pathological process of coronary heart disease by directly binding its target genes.…”

Effect of berberine on global modulation of lncRNAs and mRNAs expression profiles in patients with stable coronary heart disease

“…Abnormalities of “mTOR signaling pathway” and “MAPK signaling pathway” are associated with a variety of diseases, such as type 2 diabetes, cancer, neurodegenerative diseases, cardiovascular diseases, and autoimmune diseases [ 15 ]. Elevated serum miR-3129-5p in patients with CHD might contribute to CHD by targeting mTOR signaling [ 16 ]. Leucine induces cardioprotection in cardiac myocytes isolated from adult male Wistar rats by promoting mitochondrial function via mTOR signaling pathway [ 17 ].…”