Elevated Serum Level of MicroRNA (miRNA)-200c and miRNA-371-5p in Children with Kawasaki Disease

Abstract

MicroRNAs (miRNAs) are small, non-coding RNAs that regulate the expression of protein-coding genes. Recently, miRNA levels have been used as a novel non-invasive biomarker for the diagnosis of various diseases. We aimed to identify serum miRNAs elevated in patients with Kawasaki disease (KD) and to explore the potential biological function of identified candidate miRNAs. Serum specimens were collected from children with KD (n = 12) and healthy controls (n = 6). miRNA microarray assays were performed using the PANArray™ miRNA expression profiling kit (PANAGENE Co., Daejeon, Korea). We used TargetScan and the database for annotation, visualization, and integrated discovery program to obtain a list of enriched biological pathways targeted by miRNAs elevated in KD patients. As a result, miR-200c and miR-371-5p were significantly upregulated in the KD group compared with the control group (p = 0.032 in both). By using TargetScan, we obtained a list of 421 and 542 genes predicted to be targeted by miR-200c and miR-371, respectively, and these genes were significantly (p < 0.05) clustered in 17 and 3 pathways, respectively. Many of them are major pathways involved in inflammatory responses. The present data support the hypothesis that the inflammatory response is a crucial mechanism for pathogenesis of KD, and miRNAs might be the main regulators of this inflammatory response.

Identification of differentially expressed miRNAs through high-throughput sequencing in the chicken lung in response to Mycoplasma gallisepticum HS

“…KEGG pathway analysis facilitates understanding of the biological functions of genes (Kanehisa et al, 2008;Yun et al, 2014). In this study, KEGG pathway annotation showed that there were 23 and 25 significantly enriched pathways for these targets in phase I and phase II, respectively, and 21 pathways were shared between both time points.…”

miR-200c: a versatile watchdog in cancer progression, EMT, and drug resistance

“…Similarly, in prostate cancer, together with four other circulating miRNAs, elevated levels of miR-200c have been shown to be higher in sera taken from patients with metastatic castration-resistant prostate cancer as compared to healthy controls [132]. Dysregulated level of miR-200c in serum has also been observed in non-cancer diseases such as Crohn's disease [126] and Kawasaki disease [128] which further proves its fate as a diagnostic biomarker.…”

miR-27b Suppresses Endothelial Cell Proliferation and Migration by Targeting Smad7 in Kawasaki Disease

“…Aberrant miR-155/SOCS-1 signaling and the overexpression of miR-31 may be involved in the function of regulatory T cells, which are associated with the development of KD [24]. Another study revealed that serum miR-200c and miR-371-5p were overexpressed in KD patients [25]. In our study, serum validation and ROC curve analysis were performed and revealed that miR-27b was highly up-regulated in acute KD serum and was highly specific and sensitive for the diagnosis of KD.…”