Elevated serum haptoglobin in pigs infected with porcine reproductive and respiratory syndrome virus

Asai, Tetsuo; Mori, Masafumi; Okada, Mizue; Uruno, Katsuyoshi; Yazawa, Shigeto; Shibata, Isao

doi:10.1016/s0165-2427(99)00069-0

Cited by 78 publications

(54 citation statements)

References 17 publications

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“…Moreover, in previous field investigations, serum Hp levels were also markedly increased after exposure to the PRRSv in pigs at 8 to 10 weeks of age from a farm with chronic PRRSv infection (Asai et al, 1999). The percentage of infected pigs in the report of Asai (1999) was similar to that found in our study (85% v. 90%, respectively) at the time of Hp increment. The overall lower concentrations of APP obtained in farm 2, in comparison with the other two farms studied, could be because of the young age of animals, as it has been reported that APP in healthy animals increase with age in saliva samples (Gutié rrez et al, 2009a).…”

Section: Discussionsupporting

confidence: 90%

“…These results were similar to those obtained in experimental PRRS infections, where the highest levels of serum Hp were found at the time of seroconversion of pigs (4 times higher than those obtained at the beginning of the experiment; Díaz et al, 2005). Moreover, in previous field investigations, serum Hp levels were also markedly increased after exposure to the PRRSv in pigs at 8 to 10 weeks of age from a farm with chronic PRRSv infection (Asai et al, 1999). The percentage of infected pigs in the report of Asai (1999) was similar to that found in our study (85% v. 90%, respectively) at the time of Hp increment.…”

Section: Discussionsupporting

confidence: 88%

“…Under field conditions, APPs have been monitored in serum samples during the clinical course of PMWS (Grau-Roma et al, 2009) and in pigs chronically infected with PRRSv (Asai et al, 1999). To the authors' knowledge, our study showed for the The farms used in this study were certified as having high sanitary status, but recently had outbreaks of different viral infections: one farm had PCV2 infections, another had PRRS outbreaks and the third was seropositive to both PRRS and PCV2.…”

Section: Discussionmentioning

confidence: 81%

“…Under field conditions, APP have been monitored in pigs during the clinical course of post-weaning multisystemic wasting syndrome (PMWS; Grau-Roma et al, 2009) and in pigs with PRRS virus (PRRSv) chronic infections (Asai et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Longitudinal analysis of acute-phase proteins in saliva in pig farms with different health status

Gutiérrez

Cerón

Fuentes

et al. 2012

Animal

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This study assesses the utility of saliva samples to monitor the time course of the acute-phase response to different viruses in pigs under field conditions by using time-resolved immunofluorometric assays (TR-IFMA). A total of 30 pigs from three different farms, located in Southeast Spain, were used. Farm 1 had outbreaks of porcine circovirus type 2, farm 2 had infections with porcine reproductive and respiratory syndrome virus and farm 3 had concomitant infections with both viruses. Serology was used to determine the time of seroconversion of pigs to two different pathogens. The levels of two acute-phase proteins (APPs), C-reactive protein (CRP) and haptoglobin (Hp), were measured in saliva and serum samples and compared with pig's serology. Kinetic curves of both APPs across the study obtained in saliva samples were similar to those of serum, with R of 0.68 and 0.78 for CRP and Hp, respectively. The median CRP and Hp concentrations in saliva were higher around the theorized time of infection, according to previous experimental studies, and at seroconversion of animals. CRP increments were apparent 1 week before the increments obtained in Hp. These findings indicate that salivary APP concentrations, by using TR-IFMA, can be used in longitudinal studies as non-invasive early indicators of health status.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

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Longitudinal analysis of acute-phase proteins in saliva in pig farms with different health status

Gutiérrez

Cerón

Fuentes

et al. 2012

Animal

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“…It has often been suggested that in humans, viral infections caused a weaker response compared to that of acute bacterial infections [41]. However, not much is known about APP response elicited by viruses in pigs [2,11,35,38]. The aim of this work was to give a general view of the APP response elicited during two relevant viral infections that have been extensively studied in swine: African swine fever (ASF) and Aujeszky's disease (AD).…”

Section: Introductionmentioning

confidence: 99%

Pig Major Acute-Phase Protein and apolipoprotein A-I responses correlate with the clinical course of experimentally induced African Swine Fever and Aujeszky's disease

et al. 2007

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-In the present work, we studied the acute phase protein response after experimental virus infection in pigs. The animals were experimentally infected with African Swine Fever (ASF) or Aujeszky's disease (AD) viruses. The clinical course of ASF infection correlated with increasingly high levels of pig Major Acute-phase Protein (pig-MAP) (mean value of 6 mg/mL on day 6 post infection (p.i.), from 6 to 9 times higher than day 0) and sharp apolipoprotein A-I (apo A-I) decrease (mean value of 0.5 mg/mL, from 4 to 10 times lower than day 0 on day 4 p.i.). AD-clinical signs appeared at day 3 p.i., both in vaccinated (moderate clinical signs) and non-vaccinated pigs (severe outcome within 48 h p.i.). Pig-MAP and apo A-I profiles also followed clinical signs (changing from 0.70 mg/mL to around 3 mg/mL and from around 3 mg/mL to 0.96 mg/mL, respectively in non-vaccinated animals), with minor changes in concentration in the vaccinated group. Haptoglobin levels significantly increased in ASF and AD infected animals (mean maximum values of 2.77 and 3.96 mg/mL, respectively). Minor differences for the C-Reactive Protein in the case of ASF were observed, whereas its concentration increased more than 7 times in AD-infection. The albumin level was not modified in either case. The correlation of clinical signs to our data suggests the potential use of pig-MAP and apo A-I in monitoring infections in swine.

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Potential serum biomarkers associated with mild and severe leptospirosis infection: A cohort study in the Malaysian population

et al. 2017

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Leptospirosis is an emerging zoonotic infectious disease in Malaysia. The symptoms of leptospirosis vary from mild nonspecific flu-like illness to a severe condition which is usually associated with serious complication and fatality. To study the protein expression profile of mild and severe leptospirosis, 15 paired sera were collected from the patients who were mildly infected and following that progressed to severe stage. The proteome profiles of mild and severe cases were studied using 2DE analysis in combination with LC-MS/MS. The expression of proteins that were significantly different and had a fold difference of at least 2 had been identified and then validated using Western blot. Our study demonstrated apolipoprotein A-I (APOA-I), serum amyloid A (SAA), transferrin (TF), haptoglobin (HP) and transthyretin (TTR) have significantly different expression between mild and severe leptospirosis. The Ingenuity Pathway Analysis software suggested the expression of these five proteins were modulated by acute phase response signaling pathway. Besides that, a functional network of lipid metabolism, molecular transport and small molecule biochemistry that interconnects these five proteins with interactomes also had been predicted by this software. In conclusion, this finding supports the potential of these five proteins to be the biomarkers for mild and severe human leptospirosis.

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Elevated serum haptoglobin in pigs infected with porcine reproductive and respiratory syndrome virus

Cited by 78 publications

References 17 publications

Longitudinal analysis of acute-phase proteins in saliva in pig farms with different health status

Longitudinal analysis of acute-phase proteins in saliva in pig farms with different health status

Pig Major Acute-Phase Protein and apolipoprotein A-I responses correlate with the clinical course of experimentally induced African Swine Fever and Aujeszky's disease

Potential serum biomarkers associated with mild and severe leptospirosis infection: A cohort study in the Malaysian population

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