Elevated proton motive force is a tetracycline resistance mechanism that leads to the sensitivity to gentamicin in Edwardsiella tarda
Shao‐hua Li,
Jiao Xiang,
Ying‐yue Zeng
et al.
Abstract:Tetracycline is a commonly used human and veterinary antibiotic that is mostly discharged into environment and thereby tetracycline‐resistant bacteria are widely isolated. To combat these resistant bacteria, further understanding for tetracycline resistance mechanisms is needed. Here, GC–MS based untargeted metabolomics with biochemistry and molecular biology techniques was used to explore tetracycline resistance mechanisms of Edwardsiella tarda. Tetracycline‐resistant E. tarda (LTB4‐RTET) exhibited a globally… Show more
“…Quantitative real-time PCR (qRT-PCR) was performed as previously described with modifications [ 81 ]. Bacterial cells (1.5 mL) were harvested at OD 600 = 1.0 by centrifugation (12,000 g, 4°C, 3 min) and immediately quenched in liquid nitrogen.…”
“…Quantitative real-time PCR (qRT-PCR) was performed as previously described with modifications [ 81 ]. Bacterial cells (1.5 mL) were harvested at OD 600 = 1.0 by centrifugation (12,000 g, 4°C, 3 min) and immediately quenched in liquid nitrogen.…”
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