Elevated PLGF contributes to small-cell lung cancer brain metastasis

Li, B; Wang, C; Zhang, Y; Zhao, Xiuli; Huang, Bo; Wu, Pengfei; Li, Q; Li, H; Liu, Yongshuo; Dai, Wu; Fang, Wen-Gang; Shang, De-Shu; Cao, Ling; Zhao, Wei; Chen, Y H

doi:10.1038/onc.2012.313

Cited by 89 publications

(64 citation statements)

References 47 publications

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“…Analyses were performed using an FACSCalibur flow cytometer (BD Biosciences). Immunodetection of VEGFR-1 phosphorylation was performed by immunoprecipitation assay as described previously (19). Briefly, MSC (1 3 10 6 ) were stimulated with 10 ng/ml recombinant PlGF (R&D Systems), and cell lysates were immunoprecipitated with rabbit anti-human VEGFR-1 Ab (clone Y103, ab32152; Abcam) and control rabbit IgG.…”

Section: Analysis Of Vegfr-1 Expression and Its Phosphorylationmentioning

confidence: 99%

Interaction of Mesenchymal Stem Cells with Fibroblast-like Synoviocytes via Cadherin-11 Promotes Angiogenesis by Enhanced Secretion of Placental Growth Factor

Park

Kim

et al. 2014

The Journal of Immunology

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Bone marrow–derived mesenchymal stem cells (MSC) exist in the synovium of patients with rheumatoid arthritis (RA), yet the role of MSC in RA is elusive. Placental growth factor (PlGF) expression is increased in RA synovial fluids, and blocking of PlGF attenuates progression of arthritis in mice. In this study, we observed that PlGF induced chemotaxis of MSC in a dose-dependent manner, which was blocked by anti–vascular endothelial growth factor receptor-1 peptide. MSC exposed to PlGF elicited increased phosphorylation of Akt and p38 MAPK. PlGF-mediated chemotaxis was inhibited by PI3K inhibitor (LY294002) and p38 MAPK inhibitor (SB203580), but not by ERK1/2 inhibitor (PD98059). Fibroblast-like synoviocytes (FLS) constitutively produced PlGF, but MSC released negligible amounts of PlGF. Of note, when FLS of RA patients and MSC were cocultured, PlGF production by FLS was significantly increased; such an increase was dependent on the number of added MSC. Moreover, coculture conditioned medium promoted chemotaxis of MSC and increased angiogenesis in Matrigel plugs assay, and these were suppressed by preincubation of the medium with anti-PlGF Ab. Transwell experiments revealed that MSC to FLS contact was required for the increase in PlGF production by coculture. Cadherin-11 was expressed both in FLS and MSC, and small interfering RNA knockdown of cadherin-11 in FLS significantly abrogated the enhanced PlGF production under coculture conditions. These data indicate that increased levels of PlGF in RA joints could induce the migration of MSC to the synovium, and interaction of migrated MSC with FLS via cadherin-11 may contribute to angiogenesis and chronic synovitis by enhancing the secretion of PlGF.

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Section: Analysis Of Vegfr-1 Expression and Its Phosphorylationmentioning

confidence: 99%

Interaction of Mesenchymal Stem Cells with Fibroblast-like Synoviocytes via Cadherin-11 Promotes Angiogenesis by Enhanced Secretion of Placental Growth Factor

Park

Kim

et al. 2014

The Journal of Immunology

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“…Attention to the trajectory of instruments in the neuroforamen, 11 as well as maintaining a flexed patient posture during surgery may prevent some of these events. Previous reports have shown the rate of the dysesthesias after endoscopic foraminal decompression to be 3.5% to 6.1%, 12,13 while the rate of dysesthesias after open far lateral decompression is 6% to 24%. 14,15 Dysesthetic pain symptoms typically resolve with nonsteroidal anti-inflammatory drug, or rarely, dorsal root ganglion blockade.…”

Section: Discussionmentioning

confidence: 99%

Facet Sparing Foraminal Decompression Using the Flexible Shaver Foraminotomy System: Nerve Safety, Pain Relief, and Patient Satisfaction

et al. 2018

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Background: A number of surgical options exist for decompressing lumbar foraminal stenosis. Flexible shaver foraminotomy is a recent addition to this armamentarium. While the foraminotomy device has been incorporated into clinical practice, the literature on its safety and efficacy remain limited. We aimed to evaluate nerve safety, pain relief, and patient satisfaction in a series of patients treated with the iO-Flex shaver system (Amendia, Inc., Marietta, Georgia).Methods: Thirty-one consecutive patients with lumbar foraminal stenosis underwent foraminal decompression using the flexible microblade shaver system at 62 neuroforamina. The shavers were inserted into each foramen using an open hemilaminotomy and fluoroscopic guidance. Nerve mapping via mechanomyography (MMG) was used to ensure nerve safety. Perioperative charts were reviewed to find the incidence of neurologic complications and to quantify pain relief. Average office-based follow-up was 5.3 months. A 3-item questionnaire was administered to assess patient satisfaction during late follow-up, which occurred at an average of 21 months.Results: No planned iO-Flex foraminotomies were aborted. Neurologic complications included transient dysesthetic pain in 1 patient (3.2%, n ¼ 31), and transient numbness in 3 patients (9.7%, n ¼ 31). There were no motor deficits. The composite nerve complication rate was 12.7%. Preoperative visual analog scale scores decreased from a mean of 7.1 (n ¼ 31, standard deviation [SD] 2.0) to a mean of 3.5 (n ¼ 30, SD 2.5). If asked to repeat their decision to do surgery, 81% of patients would redo the procedure. The rate of patient dissatisfaction was 19%.Conclusions: Decompression of lumbar foramina using the flexible shaver system and MMG nerve mapping is safe and effective, although the short-term sensory complication with this technique may be higher than previously reported. Patient satisfaction with iO-Flex foraminotomy is comparable to reported satisfaction outcomes for traditional lumbar decompression.Level of Evidence: 4.

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“…Tight junction disruption is generally induced by the re-distribution of tight junction proteins such as ZO-1, claudin-5, and occludin. 6,[56][57][58] However, the GM-CSF effect on BBB permeability has remained controversial. GM-CSF mediates the lipopolysaccharide-enhanced transcellular transport of HIV-1 across BBB.…”

Section: Discussionmentioning

confidence: 99%

“…BBB properties are primarily determined by tight junctions between brain microvascular endothelial cells, which form a barrier not only against the passage of ions and molecules 2 but also T lymphocytes 5,6 and cancer cells. 7,56 Generally, the passage of cells is characterized by a lack of fully continuous tight junctions between constituent cells. Tight junction disruption is generally induced by the re-distribution of tight junction proteins such as ZO-1, claudin-5, and occludin.…”

Section: Discussionmentioning

confidence: 99%

Intracerebral GM-CSF contributes to transendothelial monocyte migration in APP/PS1 Alzheimer’s disease mice

Shang

Yang

Zhang

et al. 2016

J Cereb Blood Flow Metab

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Although tight junctions between human brain microvascular endothelial cells in the blood-brain barrier prevent molecules or cells in the bloodstream from entering the brain, in Alzheimer's disease, peripheral blood monocytes can ''open'' these tight junctions and trigger subsequent transendothelial migration. However, the mechanism underlying this migration is unclear. Here, we found that the CSF2RB, but not CSF2RA, subunit of the granulocyte-macrophage colony-stimulating factor receptor was overexpressed on monocytes from Alzheimer's disease patients. CSF2RB contributes to granulocyte-macrophage colony-stimulating factor-induced transendothelial monocyte migration. Granulocyte-macrophage colony-stimulating factor triggers human brain microvascular endothelial cells monolayer tight junction disassembly by downregulating ZO-1 expression via transcription modulation and claudin-5 expression via the ubiquitination pathway. Interestingly, intracerebral granulocyte-macrophage colony-stimulating factor blockade abolished the increased monocyte infiltration in the brains of APP/PS1 Alzheimer's disease model mice. Our results suggest that in Alzheimer's disease patients, high granulocyte-macrophage colony-stimulating factor levels in the brain parenchyma and cerebrospinal fluid induced blood-brain barrier opening, facilitating the infiltration of CSF2RB-expressing peripheral monocytes across blood-brain barrier and into the brain. CSF2RB might be useful as an Alzheimer's disease biomarker. Thus, our findings will help to understand the mechanism of monocyte infiltration in Alzheimer's disease pathogenesis.

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Elevated PLGF contributes to small-cell lung cancer brain metastasis

Cited by 89 publications

References 47 publications

Interaction of Mesenchymal Stem Cells with Fibroblast-like Synoviocytes via Cadherin-11 Promotes Angiogenesis by Enhanced Secretion of Placental Growth Factor

Interaction of Mesenchymal Stem Cells with Fibroblast-like Synoviocytes via Cadherin-11 Promotes Angiogenesis by Enhanced Secretion of Placental Growth Factor

Facet Sparing Foraminal Decompression Using the Flexible Shaver Foraminotomy System: Nerve Safety, Pain Relief, and Patient Satisfaction

Intracerebral GM-CSF contributes to transendothelial monocyte migration in APP/PS1 Alzheimer’s disease mice

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