Elevated miR-615-3p Expression Predicts Adverse Clinical Outcome and Promotes Proliferation and Migration of Prostate Cancer Cells

Laursen, Emma B.; Fredsøe, Jacob; Schmidt, Linnéa; Strand, Siri H.; Kristensen, Helle; Rasmussen, Anne; Daugaard, Tina Fuglsang; Mouritzen, Peter; Høyer, Søren; Kristensen, Gitte; Stroomberg, Hein Vincent; Brasso, Klaus; Røder, Martin Andreas; Borre, Michael; Sørensen, Karina Dalsgaard

doi:10.1016/j.ajpath.2019.08.007

Cited by 16 publications

(14 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Experiments on cell lines of various types of cancer have shown that overexpression of miR-615-3p supports cell proliferation and migration [45,46]. Functional studies carried out on PC3M prostate cancer cell lines showed that miR-615-3p promotes the proliferation, apoptosis and migration of PC3M prostate cancer cells in vitro, which indicates that miR-615-3p is a significant oncogenic microRNA in prostate cancer [47].…”

Section: Upregulated Micrornas Associated With Lymphatic Disseminatiomentioning

confidence: 99%

miRNAs expression signature potentially associated with lymphatic dissemination in locally advanced prostate cancer

et al. 2020

View full text Add to dashboard Cite

Background Prostate cancer is one of the most common and socially significant cancers among men. The aim of our study was to reveal changes in miRNA expression profiles associated with lymphatic dissemination in prostate cancer and to identify the most prominent miRNAs as potential prognostic markers for future studies. Methods High-throughput miRNA sequencing was performed for 44 prostate cancer specimens taken from Russian patients, with and without lymphatic dissemination (N1 – 20 samples; N0 – 24 samples). Results We found at least 18 microRNAs with differential expression between N0 and N1 sample groups: miR-182-5p, miR-183-5p, miR-96-5p, miR-25-3p, miR-93-5p, miR-7-5p, miR-615-3p, miR-10b, miR-1248 (N1-miRs; elevated expression in N1 cohort; p < 0.05); miR-1271-5p, miR-184, miR-222-3p, miR-221-5p, miR-221-3p, miR-455-3p, miR-143-5p, miR-181c-3p and miR-455-5p (N0-miRs; elevated expression in N0; p < 0.05). The expression levels of N1-miRs were highly correlated between each other (the same is applied for N0-miRs) and the expression levels of N0-miRs and N1-miRs were anti-correlated. The tumor samples can be divided into two groups depending on the expression ratio between N0-miRs and N1-miRs. Conclusions We found the miRNA expression signature associated with lymphatic dissemination, in particular on the Russian patient cohort. Many of these miRNAs are well-known players in either oncogenic transformation or tumor suppression. Further experimental studies with extended sampling are required to validate these results.

show abstract

Section: Upregulated Micrornas Associated With Lymphatic Disseminatiomentioning

confidence: 99%

miRNAs expression signature potentially associated with lymphatic dissemination in locally advanced prostate cancer

et al. 2020

View full text Add to dashboard Cite

show abstract

“…miRNAs are short non-coding RNAs approximately 22 nucleotides in length, which post-transcriptionally regulate gene expression of up to 60% of all human mRNAs and often play a significant role in oncogenesis [13,17,18]. Dysregulation of miRNA expression in PC has previously been demonstrated in both tumor tissue samples and liquid biopsies [19][20][21][22][23][24]. Coupled with the high stability of miRNAs in biofluids [25], this makes them particularly appealing targets as minimally invasive biomarkers.…”

Section: Introductionmentioning

confidence: 99%

Profiling of Circulating microRNAs in Prostate Cancer Reveals Diagnostic Biomarker Potential

Fredsøe

Rasmussen²,

Mouritzen³

et al. 2020

Diagnostics

Self Cite

View full text Add to dashboard Cite

Early detection of prostate cancer (PC) is paramount as localized disease is generally curable, while metastatic PC is generally incurable. There is a need for improved, minimally invasive biomarkers as current diagnostic tools are inaccurate, leading to extensive overtreatment while still missing some clinically significant cancers. Consequently, we profiled the expression levels of 92 selected microRNAs by RT-qPCR in plasma samples from 753 patients, representing multiple stages of PC and non-cancer controls. First, we compared plasma miRNA levels in patients with benign prostatic hyperplasia (BPH) or localized prostate cancer (LPC), versus advanced prostate cancer (APC). We identified several dysregulated microRNAs with a large overlap of 59 up/down-regulated microRNAs between BPH versus APC and LPC versus APC. Besides identifying several novel PC-associated dysregulated microRNAs in plasma, we confirmed the previously reported upregulation of miR-375 and downregulation of miR-146a-5p. Next, by randomly splitting our dataset into a training and test set, we identified and successfully validated a novel four microRNA diagnostic ratio model, termed bCaP (miR-375*miR-33a-5p/miR-16-5p*miR-409-3p). Combined in a model with prostate specific antigen (PSA), digital rectal examination status, and age, bCaP predicted the outcomes of transrectal ultrasound (TRUS)-guided biopsies (negative vs. positive) with greater accuracy than PSA alone (Training: area under the curve (AUC), model = 0.84; AUC, PSA = 0.63. Test set: AUC, model = 0.67; AUC, PSA = 0.56). It may be possible in the future to use this simple and minimally invasive bCaP test in combination with existing clinical parameters for a more accurate selection of patients for prostate biopsy.

show abstract

“…On the other hand, some studies on cancer cell lines have involved this miRNA in the regulation of cell growth, proliferation and migration [9], with it acting as a tumor suppressor [6] or as a tumor promoter [10][11][12].…”

Section: Functions Of Mir-615mentioning

confidence: 99%

“…miR-615-3p has been identified as a tumor suppressor in cancers including lung [18], esophageal [22] and renal [19]. In contrast, overexpression of miR-615-3p is involved in other cancers, such as hepatocellular cancer [10], gastric cancer [11] and prostate cancer [12].…”

Section: Mir-615-3p Dual Role As Oncogene and Tumor Suppressor In Cancermentioning

confidence: 99%

miR-615 Fine-Tunes Growth and Development and Has a Role in Cancer and in Neural Repair

Godínez-Rubí

Ortuño-Sahagún

2020

Cells

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are small noncoding RNAs that function as epigenetic modulators regulating almost any gene expression. Similarly, other noncoding RNAs, as well as epigenetic modifications, can regulate miRNAs. This reciprocal interaction forms a miRNA-epigenetic feedback loop, the deregulation of which affects physiological processes and contributes to a great diversity of diseases. In the present review, we focus on miR-615, a miRNA highly conserved across eutherian mammals. It is involved not only during embryogenesis in the regulation of growth and development, for instance during osteogenesis and angiogenesis, but also in the regulation of cell growth and the proliferation and migration of cells, acting as a tumor suppressor or tumor promoter. It therefore serves as a biomarker for several types of cancer, and recently has also been found to be involved in reparative processes and neural repair. In addition, we present the pleiad of functions in which miR-615 is involved, as well as their multiple target genes and the multiple regulatory molecules involved in its own expression. We do this by introducing in a comprehensible way the reported knowledge of their actions and interactions and proposing an integral view of its regulatory mechanisms.

show abstract

Elevated miR-615-3p Expression Predicts Adverse Clinical Outcome and Promotes Proliferation and Migration of Prostate Cancer Cells

Cited by 16 publications

References 45 publications

miRNAs expression signature potentially associated with lymphatic dissemination in locally advanced prostate cancer

miRNAs expression signature potentially associated with lymphatic dissemination in locally advanced prostate cancer

Profiling of Circulating microRNAs in Prostate Cancer Reveals Diagnostic Biomarker Potential

miR-615 Fine-Tunes Growth and Development and Has a Role in Cancer and in Neural Repair

Contact Info

Product

Resources

About