Elevated microRNA-21 Is a Brake of Inflammation Involved in the Development of Nasal Polyps

Liu, Ruowu; Du, Jing; Zhou, Jiao; Zhong, Bing; Ba, Luo; Zhang, Jie; Liu, Ya-Feng; Liu, Shixi

doi:10.3389/fimmu.2021.530488

Cited by 16 publications

(15 citation statements)

References 51 publications

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“… 10 Liu et al found that upregulated miR-21 can inhibit activation of NF-κB P65 and increase IL-10 expression through targeting to PDCD4 in nasal epithelial cells, which could further limit the expression of pro-inflammatory cytokines in CRSwNP. 11 Although many studies have indicated differential expressions of circRNAs and miRNAs between patients with CRSwNP and other patients or controls, the specific regulatory mechanisms through regulation of circRNA/miRNA/mRNA networks contributing to CRSwNP remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Identification of a circRNA/miRNA/mRNA ceRNA Network as a Cell Cycle-Related Regulator for Chronic Sinusitis with Nasal Polyps

Sun¹,

Liu²,

Xu³

et al. 2022

JIR

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Purpose To explore the mechanisms by which circRNA/miRNA/mRNA competitive endogenous RNAs (ceRNA) networks regulate CRSwNP. Methods The expression profiles of circRNAs, miRNAs, and mRNAs from patients with CRSwNP and control subjects were acquired from the Gene Expression Omnibus database. The circRNA/miRNA/mRNA ceRNA network was constructed based on the predicted circRNA–miRNA interactions and miRNA–mRNA interactions. Hub-mRNAs were screened by protein–protein interaction network analysis and Cytoscape molecular complex detection. The expression of factors in tissue and in hsa_circ_0031594 siRNA transfection cells was verified by RT-qPCR and the association between them was revealed by Spearman correlation analysis. Receiver operating characteristic curve analysis was performed with the pROC R package. Results The differential expression of 5423 circRNAs, 415 miRNAs, and 3673 mRNAs was identified in CRSwNP subjects compared to control subjects. Among these, 9 circRNAs, 39 miRNAs, and 78 mRNAs were screened to construct a ceRNA network. Ultimately, a subnetwork including circRNA hsa_circ_0031594, hsa-miR-1260b, hsa-miR-6507-5p, NCAPG2, RACGAP1, CHEK1 and PRC1 was screened out. RT-qPCR validated that the expression of hsa_circ_0031594, NCAPG2, PRC1 was significantly increased, and hsa-miR-1260b and hsa-miR-6507-5p were expressed significantly less in patients with CRSwNP than in control subjects. In addition, the AUCs of hsa_circ_0031594, hsa-miR-1260b, hsa-miR-6507-5p, NCAPG2, and PRC1 to discriminate CRSwNP patients were 0.995, 0.842, 0.862, 0.765, and 0.816. Spearman correlation showed that the expression of hsa_circ_0031594 was negatively correlated with hsa-miR-1260b and hsa-miR-6507-5p, and positively correlated with NCAPG2 and PRC1. In human nasal epithelial cell (HNEpC) line, knocking down hsa_circ_0031594 could increase the expression of hsa-miR-1260b and hsa-miR-6507-5p, and reduce the expression of NCAPG2 and PRC1. Conclusion CeRNA networks including hsa_circ_0031594, hsa-miR-1260b, and NCAPG2, and hsa_circ_0031594, hsa-miR-6507-5p, and PRC1 may be key regulators for CRSwNP occurrence, and may be potential targets for the pathogenesis and treatment development of CRSwNP.

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Section: Introductionmentioning

confidence: 99%

Identification of a circRNA/miRNA/mRNA ceRNA Network as a Cell Cycle-Related Regulator for Chronic Sinusitis with Nasal Polyps

Sun¹,

Liu²,

Xu³

et al. 2022

JIR

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“…In nasal epithelial cells, TGF-β1 is claimed to promote EMT via the upregulation of α-smooth muscle actin, leading to polyp formation. [ 32 ] Furthermore, Fan et al [ 16 ] revealed that miR-203a-3p is underexpressed and regulates TGF-β1-induced EMT in asthma patients. By targeting SIX1, miR-203a-3p, regulates the Smad3 pathway, thus inhibiting TGF-β1 and stopping the induction of EMT in bronchial epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs: Potential Biomarkers of Disease Severity in Chronic Rhinosinusitis with Nasal Polyps

et al. 2023

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Background and Objectives: Chronic rhinosinusitis with nasal polyps (CRwNP) has multiple clinical presentations, and predictors of successful treatment are correlated to different parameters. Differentially expressed microRNAs in nasal polyps emerge as possible facilitators of precise endotyping in this disease. We aimed to evaluate the correlation between the clinical parameters of CRSwNP and two different microRNAs. Materials and Methods: The expression of miR-125b and miR-203a-3p in nasal polyps (n = 86) and normal nasal mucosa (n = 20) was determined through microarray analysis. Preoperative workup included CT scan, nasal endoscopy, blood tests, symptoms and depression questionnaires. Results: MiR-125b showed significant overexpression in NP compared to the normal nasal mucosa. miR-125b expression levels were positively and significantly correlated with blood eosinophilia (p = 0.018) and nasal endoscopy score (p = 0.021). Although high CT scores were related to miR-125b overexpression, the correlation did not reach statistical significance. miR-203a-3p was underexpressed in nasal polyps and was significantly underexpressed in CRSwNP patients with environmental allergies. Conclusions: Both miR-125b and miR-203a-3p are potential biomarkers in CRSwNP. miR-125b also correlates with the clinical picture, while miR-203a-3p could help identify an associated allergy.

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“…Clinically, Lund‐Kennedy and Lund‐MacKay scores are commonly used to determine the disease severity of CRS patients (Bezerra et al, 2021). Because CRS comprises a wide array of mechanisms for pathophysiology, there are few markers labeling the generation and predicting the progress of CRS, which brings great difficulties to clinical diagnosis, treatment, and prognosis (Liu et al, 2021). Based on clinical data collected in this study, we analyzed Lund‐Kennedy and Lund‐MacKay scores of all CRS patients.…”

Section: Discussionmentioning

confidence: 99%

Chemerin and IL‐17 are potential predictors and Chemerin silencing alleviates inflammatory response and bone remodeling in chronic rhinosinusitis

Xie,

Bai,

Zhou

et al. 2023

Chem Biol Drug Des

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Chronic rhinosinusitis (CRS) is an inflammatory disease of paranasal sinuses. This study is formulated to explore the roles of pro‐inflammatory factors Chemerin and interleukin‐17 (IL‐17) in CRS. Patients suffering from CRS without/with nasal polyps (CRSsNP/CRSwNP), along with volunteers, were recruited. CRS rabbit models were constructed by Staphylococcus aureus infection and rabbits were injected with lentiviral vectors of short hairpin RNA‐targeting Chemerin (shChemerin), followed by micro‐computed tomography (CT) scan. Levels of Chemerin and IL‐17 were determined, and histopathological lesions were observed in subjects and CRS rabbits. Correlations between Chemerin/IL‐17 level and Lund‐Mackay/Lund‐Kennedy scores of subjects and the predictive value of Chemerin or IL‐17 for CRS were analyzed. In CRS patients and rabbits, inflammatory degrees and the level of Chemerin/IL‐17 were increased in pathological tissues or plasma, while Chemerin silencing alleviated CRS symptoms of CRS rabbits. Chemerin and IL‐17 were mainly located in the immune cells of pathological tissues and presented the positive correlation with Lund‐Mackay/Lund‐Kennedy score of CRS patients. Also, they showed high predictive value for CRS. Micro‐CT scan uncovered that CRS rabbits had increased bone remodeling, which was alleviated by Chemerin silencing. Collectively, Chemerin and IL‐17 are potential predictors and Chemerin silencing alleviates inflammatory response and bone remodeling in chronic rhinosinusitis.

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Elevated microRNA-21 Is a Brake of Inflammation Involved in the Development of Nasal Polyps

Cited by 16 publications

References 51 publications

Identification of a circRNA/miRNA/mRNA ceRNA Network as a Cell Cycle-Related Regulator for Chronic Sinusitis with Nasal Polyps

Identification of a circRNA/miRNA/mRNA ceRNA Network as a Cell Cycle-Related Regulator for Chronic Sinusitis with Nasal Polyps

MicroRNAs: Potential Biomarkers of Disease Severity in Chronic Rhinosinusitis with Nasal Polyps

Chemerin and IL‐17 are potential predictors and Chemerin silencing alleviates inflammatory response and bone remodeling in chronic rhinosinusitis

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