Elevated matrix metalloproteinase 7 expression promotes the proliferation, motility and metastasis of tongue squamous cell carcinoma

Yuan, Shi‐Min; Lin, Lisong; Gan, Rui‐Huan; Huang, Li; Wu, Xiaoting; Zhao, Yong; Su, Bogong; Zheng, Dali

doi:10.1186/s12885-020-6521-4

Cited by 21 publications

(18 citation statements)

References 42 publications

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“…MMP7 also cleaves TNF-α and other MMPs 21 . MMP7 promotes cell proliferation 22 but we observe no significant role of MMP7 in nasal cell proliferation. The absence of MMP7 protects mice from LPS-induced intestinal permeability and lethality, possibly due to MMP7 activation of β-defensins 23 .…”

Section: Discussioncontrasting

confidence: 80%

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Elevated S100A9 expression in chronic rhinosinusitis coincides with elevated MMP production and proliferation in vitro

Boruk

Railwah

Lora

et al. 2020

Sci Rep

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Chronic rhinosinusitis (CRS) is a common condition associated with inflammation and tissue remodeling of the nose and paranasal sinuses, frequently occurring with nasal polyps and allergies. Here we investigate inflammation and the protease profile in nasal tissues and plasma from control non-CRS patients and CRS patients. Gene expression for several cytokines, proteases, and antiproteases was quantified in nasal tissue from non-CRS and CRS subjects with nasal polyps. Elevated expression of S100A9, IL1A, MMP3, MMP7, MMP11, MMP25, MMP28, and CTSK was observed in tissue from CRS subjects with nasal polyps compared to control tissue. Tissue protein analysis confirmed elevated levels of these targets compared to controls, and increased MMP3 and MMP7 observed in CRS subjects with nasal polyps compared to CRS subjects without polyps. Plasma concentrations of MMP3 and MMP7 were elevated in the CRS groups compared to controls. The nasal cell line, CCL-30, was exposed to S100A9 protein, resulting in increased MMP3, MMP7, and CTSK gene expression and elevated proliferation. Silencing MMP3 significantly reduced S100A9-mediated cell proliferation. Therefore, the elevated expression of S100A9 and MMPs are observed in CRS nasal tissue and S100A9 stimulated MMP3 responses to contribute to elevated nasal cell proliferation.

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Section: Discussioncontrasting

confidence: 80%

“…Cell proliferation was measured by counting the cells in the logarithmic phase using Cell Counting Kit-8 (#CK04; Dojindo Kumamoto, Japan) as previously outlined 22 . The cells were first transfected with siRNA or plasmid and then were plated into a 96-well plate.…”

Section: Proliferation Assays the Rpmi 2650/ccl-30 (Atcc) Nasal Epitmentioning

confidence: 99%

Elevated S100A9 expression in chronic rhinosinusitis coincides with elevated MMP production and proliferation in vitro

Boruk

Railwah

Lora

et al. 2020

Sci Rep

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“…Elevated MMP-2 and MMP-7 activity has been linked to a poor prognosis in many cancers including CRC[ 27 , 28 , 38 - 47 ]. As mentioned, the impact of MICR for CRC on plasma levels of MMP-2 and MMP-7 is unknown.…”

Section: Introductionmentioning

confidence: 99%

Plasma MMP-2 and MMP-7 levels are elevated first month after surgery and may promote growth of residual metastases

Kumara¹,

Miyagaki²,

Herath³

et al. 2021

WJGO

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BACKGROUND MMP-2 also known as gelatinase A and MMP-7 (matrilysin) are members of the zinc-dependent family of MMPs (Matrix metalloproteinase). MMP-2 and MMP-7 are remodeling enzymes that digest extracellular matrix; MMP-2 is extensively expressed during development and is upregulated at sites of tissue damage, inflammation, and in stromal cells of metastatic tumors. MMP-7 is expressed in the epithelial cells and in a variety of cancers including colon tumors. Plasma MMP-2 and MMP-7 levels were assessed before and after minimally invasive colorectal resection for cancer pathology. AIM To determine plasma MMP-2 and MMP-7 levels before and after minimally invasive colorectal resection for cancer pathology. METHODS Patients enrolled in a plasma bank for whom plasma was available were eligible. Plasma obtained from preoperative (Preop) and postoperative blood samples was used. Only colorectal cancer (CRC) patients who underwent elective minimally invasive cancer resection with preop, post-operative day (POD) 1, 3 and at least 1 late postop sample (POD 7-34) were included. Late samples were bundled into 7 d blocks (POD 7-13, 14-20, etc. ) and treated as single time points. Plasma MMP-2 and MMP-7 levels were determined via enzyme-linked immunosorbent assay in duplicate. RESULTS Total 88 minimally invasive CRC resection CRC patients were studied (right colectomy, 37%; sigmoid, 24%; and LAR/AR 18%). Cancer stages were: 1, 31%; 2, 30%; 3, 34%; and 4, 5%. Mean Preop MMP-2 plasma level (ng/mL) was 179.3 ± 40.9 ( n = 88). Elevated mean levels were noted on POD1 (214.3 ± 51.2, n = 87, P < 0.001), POD3 (258.0 ± 63.9, n = 80, P < 0.001), POD7-13 (229.9 ± 62.3, n = 65, P < 0.001), POD 14-20 (234.9 ± 47.5, n = 25, P < 0.001), POD 21-27 (237.0 ± 63.5, n = 17, P < 0.001,) and POD 28-34 (255.4 ± 59.7, n = 15, P < 0.001). Mean Preop MMP-7 level was 3.9 ± 1.9 ( n = 88). No significant differences were noted on POD 1 or 3, however, significantly elevated levels were noted on POD 7-13 (5.7 ± 2.5, n = 65, P < 0.001), POD 14-20 (5.9 ± 2.5, n = 25, P < 0.001), POD 21-27 (6.1 ± 3.6, n = 17, P = 0.002) and on POD 28-34 (6.8 ± 3.3, n = 15 P < 0.001,) vs preop levels. CONCLUSION MMP-2 levels are elevated for 5 wk and MMP-7 levels elevated for weeks 2-...

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“…MMP-2 knockdown was shown to suppress the proliferative capacity of hepatocellular carcinoma cells in vitro [ 26 ]. MMP-7 was shown to promote the proliferation of mouse-derived tongue squamous cell carcinoma [ 27 ]. MMP-7 was found to be directly involved in cell survival, proliferation, migration and invasion of the cervical cancer cells, and its expression was suggested as a novel prognostic biomarker [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Bioinformatics and Molecular Insights to Anti-Metastasis Activity of Triethylene Glycol Derivatives

Malik

Garg

Afzal

et al. 2020

IJMS

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The anti-metastatic and anti-angiogenic activities of triethylene glycol derivatives have been reported. In this study, we investigated their molecular mechanism(s) using bioinformatics and experimental tools. By molecular dynamics analysis, we found that (i) triethylene glycol dimethacrylate (TD-10) and tetraethylene glycol dimethacrylate (TD-11) can act as inhibitors of the catalytic domain of matrix metalloproteinases (MMP-2, MMP-7 and MMP-9) by binding to the S1’ pocket of MMP-2 and MMP-9 and the catalytic Zn ion binding site of MMP-7, and that (ii) TD-11 can cause local disruption of the secondary structure of vascular endothelial growth factor A (VEGFA) dimer and exhibit stable interaction at the binding interface of VEGFA receptor R1 complex. Cell-culture-based in vitro experiments showed anti-metastatic phenotypes as seen in migration and invasion assays in cancer cells by both TD-10 and TD-11. Underlying biochemical evidence revealed downregulation of VEGF and MMPs at the protein level; MMP-9 was also downregulated at the transcriptional level. By molecular analyses, we demonstrate that TD-10 and TD-11 target stress chaperone mortalin at the transcription and translational level, yielding decreased expression of vimentin, fibronectin and hnRNP-K, and increase in extracellular matrix (ECM) proteins (collagen IV and E-cadherin) endorsing reversal of epithelial–mesenchymal transition (EMT) signaling.

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Elevated matrix metalloproteinase 7 expression promotes the proliferation, motility and metastasis of tongue squamous cell carcinoma

Cited by 21 publications

References 42 publications

Elevated S100A9 expression in chronic rhinosinusitis coincides with elevated MMP production and proliferation in vitro

Elevated S100A9 expression in chronic rhinosinusitis coincides with elevated MMP production and proliferation in vitro

Plasma MMP-2 and MMP-7 levels are elevated first month after surgery and may promote growth of residual metastases

Bioinformatics and Molecular Insights to Anti-Metastasis Activity of Triethylene Glycol Derivatives

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