Elevated levels of synovial fluid PLA₂, strome-lysin (MMP-3) and TIMP in early osteoarthrosis after tibial valgus osteotomy in young beagle dogs

Decline after immobilisation and recovery after remobilisation of synovial fluid IL1, TIMP, and chondroitin sulphate levels in young beagle dogs

“…These observations are in contrast with those obtained after induction of canine osteoarthritis by tibial valgus osteotomy 88. The assays were the same as used in this study.…”

“…3 Tissue inhibitor of metalloproteinases (TIMP) inhibits the activity of matrix metalloproteinases 4-6. Increased concentrations of metalloproteinases, and their tissue inhibitors have been detected in human osteoarthritis, in rheumatoid arthritis,7 and in early experimental osteoarthritis induced in beagles by tibial osteotomy 8. The activation of metalloproteinases can be initiated by cytokines such as interleukin 1 (IL1), whereas IL6 induces the synthesis of TIMP-1 but not of the metalloproteinases 9…”

“…Interest was focused on changes which would allow comparison between the synovial fluid marker changes in immobilisation-induced atrophy and experimental osteoarthritis 8. The effects of increased weight bearing on the contralateral limb were also investigated.…”

Assessment of cellular, biochemical, and histologic effects of bipolar radiofrequency treatment of canine articular cartilage

“…Although this may be indicative of tissue remodeling, it seems more likely that the cells that were not killed had nevertheless undergone insult that resulted in a negative shift in the balance between synthesis and degradation of the extracellular matrix. [33][34][35][36][37][38] Regardless of whether the increased MMP-13 activity detected was a direct result of the radiofrequency treatment or a sequela to radiofrequency-induced changes in tissue permeability, this response could be potentially devastating because radiofrequency treatment may enhance rather than slow the progression of osteoarthritis.…”

Isolation, characterization, and expression of stromelysin-1 in primary tumors of dogs

“…[2][3][4] Physiologic processes affected by stromelysin-1 activity include cartilage repair, 5,6 uveoscleral outflow, 7 controlled inflammatory processes, 8 menstruation, 9 parturition, 10 cell adhesion, 11 wound repair, 11 and cell migration. 12 Increased activity of stromelysin-1 increases the pathogenicity of various conditions, including benign and malignant neoplasias, 1,13-16 diabetes mellitus, 17 viral meningitis, 18 coronary artery disease, 19,20 cutis laxa, 21 hepatic cirrhosis, 22 osteoarthritis, 23,24 rheumatoid arthritis, 25,26 Crohn' s disease, 27 glomerular nephritis disease, 28 infection with Epstein-Barr virus, 29 response to damage caused by UV radiation, 30 chronic ulcers, 31 and autoimmune diseases. 32 Neoplasms that express stromelysin-1 in humans include carcinomas of the breast, prostate, squamous epithelium, liver, and biliary system as well as melanoma and bone cancer.…”