Elevated levels of serum antibodies against alpha-1, 6-glucan in patients with systemic lupus erythematosus or rheumatoid arthritis

Dai, Hui; Gao, Xiao‐Ming

doi:10.1007/s13238-011-1095-1

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…However, of studies on the diagnostic value, Ag specificity, and pathogenic potential of autoantibodies correlated to the development of RA in humans, very few touched upon glycanspecific autoantibodies (20)(21)(22), even though carbohydrate structures represent a substantial portion of tissue Ags in the joints and could be targeted, just like any other autoantigens, by arthritogenic autoantibodies. Using polysaccharide-based ELISAs, we have previously found positive correlation between RA and serum Abs against dextran and mannan (23,24). In our more recent screening assays, an even stronger correlation between RA and circulating Abs against pectin and a1,4-polygalacturonic acid [(PGA); major structural component of pectin] was found.…”

mentioning

confidence: 73%

Disease Association and Arthritogenic Potential of Circulating Antibodies against the α1,4-Polygalacturonic Acid Moiety

Dai

Dong

Gong

et al. 2014

The Journal of Immunology

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Much progress has been made in recent years on the diagnostic value, Ag specificity, and pathogenic roles of autoantibodies correlated to the development of rheumatoid arthritis (RA) in humans. However, carbohydrate Ag-specific autoantibodies that may also play important roles in RA have largely been ignored. In this article, we report that serum levels of Abs capable of recognizing α1,4-polygalacturonic acid [(PGA); major structural component of pectin] strongly correlate with RA in humans. The measurements of PGA-specific Abs (PGA-Abs) in sera are comparable to rheumatoid factors and anti–cyclic citrullinated peptide Abs as serological diagnostic markers for RA in terms of sensitivity and specificity. Immunohistochemical staining results indicate that the PGA-Abs selectively bound synovial membrane cells and chondrocytes in the joints of both humans and rabbits (but not rodents). Induction of PGA-Abs by s.c. immunization of rabbits with carrier protein–conjugated synthetic PGA led to severe inflammatory reactions (synovial hyperplasia, small vessel proliferation, and inflammatory cell infiltration) in the joints. Injection of affinity purified anti-PGA IgG into the synovial cavity of rabbits resulted in accumulation of proinflammatory cytokines such as TNF-α, IL-8, and IL-1β in synovial fluid, as well as local pathological damage. We conclude that the PGA–cross-reactive moiety represents a major autoantigen in the joints and can be targeted by autoantibodies capable of triggering arthritogenic responses in vivo.

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confidence: 73%

Disease Association and Arthritogenic Potential of Circulating Antibodies against the α1,4-Polygalacturonic Acid Moiety

Dai

Dong

Gong

et al. 2014

The Journal of Immunology

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“…Sensitivity of this test was 64.81%, and specificity 84.62% ( 106 ). Anti-alpha-1,6-glucan-IgG levels were significantly elevated in patients with SLE and the sensitivity for detecting SLE was 93.3%, whereas the specificity was 73.3% and the area under the ROC curve was 0.863 ( 12 ). Antibodies to cell membrane associated DNA (mDNA) were identified by an indirect immunofluorescence assay using a B cell line fixed but not permeabilized with sensitivity of 65% and specificity of 98% ( 107 ).…”

Section: Biomarkers In Slementioning

confidence: 98%

Emerging Molecular Markers Towards Potential Diagnostic Panels for Lupus

et al. 2022

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Systemic lupus erythematosus (SLE) is a multifactorial autoimmune disease which can affect various tissues and organs, posing significant challenges for clinical diagnosis and treatment. The etiology of SLE is highly complex with contributions from environmental factors, stochastic factors as well as genetic susceptibility. The current criteria for diagnosing SLE is based primarily on a combination of clinical presentations and traditional lab testing. However, these tests have suboptimal sensitivity and specificity. They are unable to indicate disease cause or guide physicians in decision-making for treatment. Therefore, there is an urgent need to develop a more accurate and robust tool for effective clinical management and drug development in lupus patients. It is fortunate that the emerging Omics have empowered scientists in the discovery and identification of potential novel biomarkers of SLE, especially the markers from blood, urine, cerebrospinal fluids (CSF), and other bodily fluids. However, many of these markers have not been carefully validated for clinical use. In addition, it is apparent that individual biomarkers lack sensitivity or specificity. This review summarizes the sensitivity, specificity and diagnostic value of emerging biomarkers from recent studies, and discusses the potential of these markers in the development of biomarker panel based diagnostics or disease monitoring system in SLE.

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“…Noteworthy, also in the case of SLE and AR diseases the sera antibodies were found to target exogenous agents-derived glycans [39][40][41].…”

Section: Mechanism Investigations and Clinical Relevance Of Molecularmentioning

confidence: 99%

Glycoreplica peptides to investigate molecular mechanisms of immune-mediated physiological versus pathological conditions

Mazzoleni

Mallet

Rovero

et al. 2019

Archives of Biochemistry and Biophysics

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Investigation of the role of saccharides and glycoconjugates in mechanisms of immune-mediated physiological and pathological conditions is a hot topic. In fact, in many autoimmune diseases crossreactivity between sugar moieties exposed on exogenous pathogens and self-molecules has long been hinted. Several peptides have been reported as mimetics of glycans specifically interacting with sugar-binding antibodies. The seek for these glycoreplica peptides is instrumental in characterizing antigen mimicry pathways and their involvement in triggering autoimmunity. Therefore, peptides mimicking glycan-protein interactions are valuable molecular tools to overcome the difficulties of oligosaccharide preparations. The clinical impact of peptide-based probes for autoimmune diseases diagnosis and follow-up is emerging only recently as just the tip of the iceberg of an overlooked potential. Here we provide a brief overview of the relevance of the structural and functional aspects of peptide probes and their mimicry effect in autoimmunity mechanisms for promising applications in diagnostics and therapeutics.

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Elevated levels of serum antibodies against alpha-1, 6-glucan in patients with systemic lupus erythematosus or rheumatoid arthritis

Cited by 5 publications

References 21 publications

Disease Association and Arthritogenic Potential of Circulating Antibodies against the α1,4-Polygalacturonic Acid Moiety

Disease Association and Arthritogenic Potential of Circulating Antibodies against the α1,4-Polygalacturonic Acid Moiety

Emerging Molecular Markers Towards Potential Diagnostic Panels for Lupus

Glycoreplica peptides to investigate molecular mechanisms of immune-mediated physiological versus pathological conditions

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