Elevated Levels of CYP94 Family Gene Expression Alleviate the Jasmonate Response and Enhance Salt Tolerance in Rice

Abstract

The plant hormone jasmonate and its conjugates (JAs) have important roles in growth control, leaf senescence and defense responses against insects and microbial attacks. JA biosynthesis is induced by several stresses, including mechanical wounding, pathogen attacks, drought and salinity stresses. However, the roles of JAs under abiotic stress conditions are unclear. Here we report that increased expression of the Cyt P450 family gene CYP94C2b enhanced viability of rice plants under saline conditions. This gene encodes an enzyme closely related to CYP94C1 that catalyzes conversion of bioactive jasmonate-isoleucine (JA-Ile) into 12OH-JA-Ile and 12COOH-JA-Ile. Inactivation of JA was facilitated in a rice line with enhanced CYP94C2b expression, and responses to exogenous JA and wounding were alleviated. Moreover, salt stress-induced leaf senescence but not natural senescence was delayed in the transgenic rice. These results suggest that bioactive JAs have a negative effect on viability under salt stress conditions and demonstrate that manipulating JA metabolism confers enhanced salt tolerance in rice.

RNAseq analysis reveals pathways and candidate genes associated with salinity tolerance in a spaceflight-induced wheat mutant

“…CYPs catalyze the biooxidation of various substrates through activation of molecular oxygen and play important roles in metabolic processes and stress responses 54 . It has been suggested that increased expression of CYP94 genes in rice plants alleviates jasmonate responses and enhances salt tolerance 55 . Mutation of CYP709B3 in Arabidopsis conferred sensitivity to ABA and low tolerance to salt stress, indicating that CYP709B3 plays a role in ABA responses and salinity tolerance 56 .…”

A chloroplast‐localized protein LESION AND LAMINA BENDING affects defence and growth responses in rice

“…The pathway is modified from Arabidopsis, and genes that have been functionally confirmed or proposed in rice are shown in blue. Os OPR 7/Os08g0459600 (Tani et al ., ), Os JMT 1/Os05g0102000 (Qi et al ., ), Os JAR 1/Os05g0586200 and Os JAR 2/Os01g0221100 (Wakuta et al ., ), and CYP 94C2b/Os12g0150200 (Kurotani et al ., ). (b) OPDA , JA , and JA ‐Ile contents of 6‐wk‐old glasshouse‐grown wild‐type ( WT ) and llb plants.…”

“…Loss of LLB function resulted in a pleiotropic phenotype including increased hypersensitive response (HR)-like lesion formation and lamina bending, constitutive expression of stress/defence related genes, enhanced resistance to fungal and bacterial pathogens, elevated jasmonic acid (JA) and brassinosteroid (BR) accumulation/signalling, The pathway is modified from Arabidopsis, and genes that have been functionally confirmed or proposed in rice are shown in blue. OsOPR7/Os08g0459600 (Tani et al, 2008), OsJMT1/Os05g0102000 (Qi et al, 2015), OsJAR1/ Os05g0586200 and OsJAR2/Os01g0221100 , and CYP94C2b/Os12g0150200 (Kurotani et al, 2015). (b) OPDA, JA, and JA-Ile contents of 6-wk-old glasshouse-grown wild-type (WT) and llb plants.…”

OsCYP71D8L as a key regulator involved in growth and stress response by mediating gibberellins and cytokinins homeostasis in rice

“…Rice growth and development was affected by adverse environment. Overexpression of PtCYP714A3, OsDSS1 and CYP94C2b could improve rice tolerance to drought and salt stress (Kurotani et al, 2015; Tamiru et al, 2015; Wang et al, 2016). Here, we confirmed that OsCYP71D8L conferred rice with better tolerance to drought and salinity stress.…”