Elevated levels of circulating platelet microparticles, VEGF, IL-6 and RANTES in patients with gastric cancer: possible role of a metastasis predictor

Kim, H.K; Song, Kyung Soon; Park, Y.S; Kang, Young-Ah; Lee, Y.J; Lee, K.R; Ryu, Keun Won; Bae, Jae-Moon; Kim, S

doi:10.1016/s0959-8049(02)00596-8

Cited by 360 publications

(260 citation statements)

References 29 publications

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“…In our experiments, we employed doses of PMV that we considered to be clinically relevant. 5,53 Our major finding is that PMV derived from activated platelets interact with lung cancer cells and enhance their metastatic and angiogenic potential. PMV chemoattracted 5 out of the 6 human and murine lung cancer cell lines studied, with the strongest effects shown by the highly metastatic human A549 cell line and the murine LCC cells.…”

Section: Discussionmentioning

confidence: 99%

“…Further supporting our data is a recent report showing that the increased level of PMV circulating in PB is a strong predictor of metastasis in patients with gastric cancer. 53 More studies, however, are needed to identify the specific PMV components that exert these biologic effects, as a better understanding of the role PMV play in tumor progression and metastasis could help us to develop novel therapeutic approaches.…”

Section: Discussionmentioning

confidence: 99%

“…The concentrations of both the PMV and the exosomes were estimated by Bradford assay. As it has been reported that the concentration of PMV in peripheral blood as measured by protein concentration is about 5 g/mL 5 and can be elevated up to 10 times in cancer patients, 53 the doses of PMV we employed in the experiments described below were within the clinically relevant range.…”

Section: Human and Murine Pmv And Exosomesmentioning

confidence: 99%

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Microvesicles derived from activated platelets induce metastasis and angiogenesis in lung cancer

Janowska‐Wieczorek

Wysoczynski

Kijowski

et al. 2004

Intl Journal of Cancer

666

493

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Human and Murine Pmv And Exosomesmentioning

confidence: 99%

See 1 more Smart Citation

Microvesicles derived from activated platelets induce metastasis and angiogenesis in lung cancer

Janowska‐Wieczorek

Wysoczynski

Kijowski

et al. 2004

Intl Journal of Cancer

666

493

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“…Tumor cells release large amount of MVs, and the number of circulating MVs is increased in patients with cancer and correlates with poor prognosis. 67 This may depend on the pleyotropic effects of MVs. Thrombotic events are common among different cancer types and stages.…”

Section: Conflict Of Interestmentioning

confidence: 99%

The role of microvesicles in tissue repair

Bruno²,

et al. 2011

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“…Indeed, increased levels of PMPs were demonstrated in the circulation of patients with different cancers. 11 PMPs express platelet receptors on their surface and (along with exosomes, i.e., granule-derived vesicles) contain regulatory compounds from platelet granules. It has recently been reported that PMPs promote metastasis of lung and breast cancers, 12,13 a process in which MMPs were considered to play a central role.…”

mentioning

confidence: 99%

Platelet‐derived microparticles promote invasiveness of prostate cancer cells via upregulation of MMP‐2 production

Dashevsky

Varon

Brill

2009

Intl Journal of Cancer

124

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Prostate cancer commonly affects men in the Western world. A major factor of the life-threatening course of this disease is the high rate of metastasis, predominantly to bones. Circulating tumor cells encounter platelets and may activate them, resulting in a production of microparticles (MPs). MPs are small platelet fragments expressing membrane receptors as well as cytoplasmic constituents. Here, we report that prostate cancer cells, Clone-1 (Cl-1), preincubated with platelet-derived MPs (PMPs), demonstrate increased invasion through a gelatin-coated (a denatured form of collagen) membrane of the Boyden chamber system. This effect was accompanied by an increased secretion of metalloproteinase-2 (MMP-2) as demonstrated by a gelatin zymography. Application of MMP-2/9 inhibitor reversed the PMP-induced tumor cell invasion. PMPs were shown to adhere to Cl-1 cells, but direct contact between them may not be mandatory for MMP secretion because PMP lysate induced MMP-2 production by Cl-1 cells to the same extent as did intact PMPs. PMP-induced MMP-2 secretion was inhibited by neutralization of either PKC or total intracellular tyrosine phosphorylation, but was not affected by blocking major intraplatelet cytokines. Actinomycin D (a transcription inhibitor) did not modify this effect, whereas cycloheximide (an inhibitor of protein translation) abolished the MMP-2 release. MMP-2 secretion was accompanied by a rapid and transient increase in MMP-2 mRNA level after a 2-hr coincubation of prostate cancer cells with PMPs. Thus, PMPs promote tumor invasiveness, at least in part by stimulation of MMP-2 production. ' 2008 Wiley-Liss, Inc.Key words: platelet-derived microparticles; prostate cancer; MMP-2 Platelet involvement in dissemination of tumor metastasis was first experimentally demonstrated several decades ago. 1 Potential mechanisms of the platelet effect on metastatic dissemination include PAR receptors, platelet-derived lysophosphatidic acid and others. 2,3 Platelets were previously shown to directly mediate tumor cell adhesion to the vascular wall thus facilitating their future egress into tissues. 4 However, functional platelet depletion several weeks after tumor inoculation also results in reduction of metastasis, 3 an effect that cannot be explained by decreased tumor cell survival or extravasation. In addition, platelets may serve as a source of numerous proangiogenic cytokines that support growth of new capillaries in the vicinity of metastasis. 5 Angiogenesis is a prerequisite for tumor growth. 6 The ability of platelets as a cellular system to induce angiogenesis in spite of numerous antiangiogenic compounds in their alpha-granules has recently been demonstrated. 7,8 Matrix metalloproteinases (MMPs) belong to a family of zinccontaining enzymes that are tightly involved in the angiogenic process as well as in tumor cell extravasation and metastasis. 9 Among this group of endopeptidases, MMP-2 and MMP-9 are considered most important for metastatic spread. Both enzymes cleave collagen that constitutes a major co...

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Elevated levels of circulating platelet microparticles, VEGF, IL-6 and RANTES in patients with gastric cancer: possible role of a metastasis predictor

Cited by 360 publications

References 29 publications

Microvesicles derived from activated platelets induce metastasis and angiogenesis in lung cancer

Microvesicles derived from activated platelets induce metastasis and angiogenesis in lung cancer

The role of microvesicles in tissue repair

Platelet‐derived microparticles promote invasiveness of prostate cancer cells via upregulation of MMP‐2 production

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