Elevated Levels of a Disintegrin and Metalloproteinase 8 in Gingival Crevicular Fluid of Patients With Periodontal Diseases

Abstract

The ADAM8 levels are elevated in the GCF of patients with periodontal diseases, including gingivitis, CP, and AgP, in comparison to control participants who are healthy, and they correlate with four clinical parameters that reflect the degree of disease severity.

Inducible expression of A Disintegrin and Metalloproteinase 8 in chronic periodontitis and gingival epithelial cells

“…Because significantly elevated ADAM8 levels in gingival crevicular fluid collected from patients with chronic periodontitis have previously been shown , ADAM8 mRNA and protein expression was determined in gingival biopsies from 33 patients with chronic periodontitis compared with that from 23 healthy volunteers. The degrees of ADAM8 mRNA expression varied among different individuals in both the chronic periodontitis and normal groups (Fig.…”

“…Subsequent to written informed consent obtained from all participants, surgical biopsies were undertaken at the Oral and Maxillofacial Surgery Clinic, Faculty of Dentistry, Chiang Mai University, to collect gingival tissues (~3 × 3 mm) surrounding unsalvageable teeth planned for tooth extraction of 33 patients (age range 41–63 years), diagnosed with chronic periodontitis according to the criteria of the American Academy of Periodontology, 1999 , and normal non‐inflamed gingival tissues overlying bony impaction of third molars from 23 healthy volunteers (age range 23–32 years). The exclusion criteria for participants were as previously described , including diabetes mellitus, rheumatoid arthritis, osteoarthritis, osteoporosis, smoking, febrile illnesses, oral lesions, orthodontic and periodontal treatment, pregnancy and lactation, and antibiotic, anti‐inflammatory or immunosuppressive drug use within 3 mo before sample collection. The research protocol (no.…”

“…Elevated levels of ADAM8 are detected in serum, bronchoalveolar lavage fluid and amniotic fluid in association with several pathological conditions, including rheumatoid arthritis, asthma, pre‐term delivery, pneumonia, allergy and myocardial infarction . With respect to periodontal disease, significantly elevated levels of ADAM8 have been recently demonstrated in gingival crevicular fluid collected from patients with chronic periodontitis . Consequently, it was hypothesized in this study that ADAM8 expression in gingival biopsies of patients with chronic periodontitis would be increased when compared with that in normal non‐inflamed gingival tissues.…”

“…The resulting uncontrolled inflammatory responses and failure to resolve inflammation can lead to overproduction of cytokines, chemokines and other effector molecules of innate immunity, such as matrix metalloproteinases that belong to the metzincin superfamily, whose levels are elevated in gingival crevicular fluid of patients with periodontitis . Similarly, the levels of the other two members of the metzincin superfamily, including A Disintegrin and Metalloproteinase (ADAM) 17 or tumor necrosis factor (TNF)‐α‐converting enzyme and ADAM8 , are also shown to be elevated in gingival crevicular fluid collected from periodontitis sites, indicating the importance of these two ADAMs in the pathogenesis of periodontitis.…”

Classification and diagnosis of aggressive periodontitis

“…Twelve current studies related to local host responses in AgP were examined (Table 3). 30,[41][42][43][44][45][46][47][48][49][50][51] Of these, 9 studies 41,42,44-46,49-52 looked at multiple crevice sites within a patient population. Of these, 5 manuscripts 46,[48][49][50]52 reported multiple mediators at the local site.…”