Elevated level of 17β-estradiol is associated with overexpression of FSHR, CYP19A1, and CTNNB1 genes in porcine ovarian follicles after prenatal and neonatal flutamide exposure

Grzesiak, Małgorzata; Knapczyk-Stwora, Katarzyna; Duda, Małgorzata; Słomczyńska, Maria

doi:10.1016/j.theriogenology.2012.07.026

Cited by 25 publications

(17 citation statements)

References 39 publications

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“…CYP19A1 and HSD17B1 are responsible for estrogen biosynthesis. After prenatal and neonatal flutamide exposure in porcine ovarian follicles, the elevated level of 17-estradiol is associated with overexpression of the follicle stimulating hormone receptor (FSHR), CYP19A1, and CTNNB1 genes [57]. However, despite the role of the key enzymes of the steroid synthesis pathway, steroid hormones have a negative feedback effect on steroidogenic enzyme expression and regulate their own production in ovarian tissue [58], [59].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling Identifies Differentially Expressed Genes in Huoyan Goose Ovaries between the Laying Period and Ceased Period

Liu

Cao

et al. 2014

PLoS ONE

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The Huoyan goose is famous for its high egg-laying performance and is listed as a nationally protected domestic animal by the Chinese government. To elucidate the key regulatory genes involved in Huoyan goose egg laying, RNA from ovarian tissue during the ceased and laying periods was sequenced using the Illumina HiSeq 2000 sequencing platform. More than 12 million reads were produced in ceased and laying libraries that included 11,896,423 and 12,534,799 clean reads, respectively. More than 20% of the reads were matched to the reference genome, and 23% of the reads were matched to reference genes. Genes with a false discovery rate (FDR) ≤0.001 and log2ratio ≧1 or ≤−1 were characterized as differentially expressed, and 344 up-regulated and 344 down-regulated genes were classified into functional categories. Twelve genes that are mainly involved in pathways for reproduction regulation, such as steroid hormone biosynthesis, GnRH signaling pathways, oocyte meiosis, progesterone-mediated oocyte maturation, steroid biosynthesis, calcium signaling pathways, and G-protein coupled receptor signaling pathway were selected for validation by a quantitative real-time polymerase chain reaction (qRT-PCR) analysis, the qRT-PCR results are consistent with the general expression patterns of those genes from the Illumina sequencing. These data provide comprehensive gene expression information at the transcriptional level that might increase our understanding of the Huoyan goose's reproductive biology.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling Identifies Differentially Expressed Genes in Huoyan Goose Ovaries between the Laying Period and Ceased Period

Liu

Cao

et al. 2014

PLoS ONE

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“…; Grzesiak et al. ). Therefore, foetal androgen deficiency has striking effects on adult ovarian function.…”

Section: Introductionmentioning

confidence: 98%

“…, ; Grzesiak et al. ), it is notable that disturbed androgens action during early folliculogenesis affects female fertility in adulthood. In pigs, we have shown that administration of the anti‐androgen flutamide within critical windows of foetal ovarian development led to altered follicular steroidogenesis as well as luteinization and corpus luteum function after reaching sexual maturation (Durlej et al.…”

Section: Introductionmentioning

confidence: 99%

Effect of Prenatal and Neonatal Anti‐Androgen Flutamide Treatment on Aquaporin 5 Expression in the Adult Porcine Ovary

Grzesiak

Knapczyk-Stwora

Mobasheri

et al. 2015

Reprod Domestic Animals

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The growth of ovarian follicles is accompanied by fluid-filled antrum formation. Water movement within the follicular wall is predominantly transcellular via membranous water channels named aquaporins (AQPs). Androgens are important regulators of mammalian folliculogenesis, and their prenatal and/or neonatal deficiency affects female fertility in adulthood. Therefore, this study was performed to determine whether gestational or neonatal exposure to the anti-androgen flutamide influences androgen-dependent AQP5 expression in pre-antral and large antral follicles of adult pigs. Flutamide was injected into pregnant gilts between days 80 and 88 of gestation and into female piglets between days 2 and 10 post-natally. The ovaries were collected from flutamide-treated and non-treated (control) sexually mature pigs. In pre-antral follicles, AQP5 mRNA and protein levels were both downregulated following maternal (p < 0.01 and p < 0.01, respectively) and neonatal (p < 0.01 and p < 0.01, respectively) flutamide exposure. Likewise, the expression of mRNA (p < 0.01 and p < 0.001, respectively) and protein (p < 0.05 and p < 0.01, respectively) for AQP5 were diminished in large antral follicles in both groups. Immunohistochemistry showed decreased intensity of AQP5 immunoreaction in pre-antral (p < 0.01) and large antral (p < 0.001) follicles following flutamide treatment. Moreover, radioimmunological analysis revealed that changes observed in AQP5 expression corresponded with diminished follicular androgens production after both maternal (p < 0.05 and p < 0.05, respectively) and neonatal (p < 0.05 and p < 0.01, respectively) flutamide administration. Therefore, AQP5 appears to be a potential regulator of follicular fluid accumulation, under androgen control, and may be a key factor in antral follicle growth.

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“…Abnormal folliculogenesis was also seen in prepubertal pigs, following prenatal period of anti-androgen exposure [60], whereas in adult animals, only neonatal time of flutamide treatment exerted disturbances marked by altered luteinization and corpus luteum cyst formation [61]. Apart from morphological disturbances, the altered follicular functioning in adulthood was manifested by changes in production of steroids (estradiol and androgens) and gonadotropins (FSH and LH), as well as in expression of genes involved in proper ovarian functioning, including AR, FSHR, aromatase, connexin 43, β-catenin, and aquaporin 5 [61][62][63]. Furthermore, gestational or neonatal exposure to flutamide affected proliferation and apoptosis rates in large antral follicles of adult porcine ovary, which might influence the normal development of the follicles and pigs fertility as a consequence [64].…”

Section: Eacs With Androgenic and Anti-androgenic Propertiesmentioning

confidence: 99%

Endocrine Active Compounds Actions during Neonatal Period: Effect on the Ovary

Słomczyńska¹,

Grzesiak²,

Stwora³

2018

Selected Topics in Neonatal Care

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Many female reproductive disorders observed during adulthood originate from the neonatal period, which is a critical stage toward the reproductive potency. Human and animal fertility are determined by the pool of primordial follicles that are established during fetal or neonatal life. The earliest stages of follicle development are under control of a variety of factors, including sex steroids. Neonatal period is a "critical developmental window" in which organisms are susceptible to the environmental chemicals that may affect the reproductive health. Endocrine active compounds (EACs) are found abundantly in the environment and interfere with sex steroids (predominantly androgens and estrogens) by either mimicking or blocking their functions. This review covers the current knowledge about the effects of selected EACs with androgenic (testosterone propionate), anti-androgenic (flutamide), estrogenic (diethylstilbestrol, bisphenol A, 4-tert-octylphenol, phtalates and genistein), anti-estrogenic (ICI 182,780 and parabens), or mixed activity (methoxychlor) on the ovary of neonates, focusing on their effects on the early stages of folliculogenesis. These chemicals have been shown to affect oocyte survival, follicle formation, and growth, as well as steroidogenic functions. The better cognition of mechanisms underlying the long-term consequences of the neonatal EACs exposure may in future lead to an understanding of human health risks and developing prevention strategies.

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Elevated level of 17β-estradiol is associated with overexpression of FSHR, CYP19A1, and CTNNB1 genes in porcine ovarian follicles after prenatal and neonatal flutamide exposure

Cited by 25 publications

References 39 publications

Transcriptome Profiling Identifies Differentially Expressed Genes in Huoyan Goose Ovaries between the Laying Period and Ceased Period

Transcriptome Profiling Identifies Differentially Expressed Genes in Huoyan Goose Ovaries between the Laying Period and Ceased Period

Effect of Prenatal and Neonatal Anti‐Androgen Flutamide Treatment on Aquaporin 5 Expression in the Adult Porcine Ovary

Endocrine Active Compounds Actions during Neonatal Period: Effect on the Ovary

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