Elevated Interleukin‐8 in Cervical Mucus as an Indicator for Treatment to Prevent Premature Birth and Preterm, Pre‐labor Rupture of Membranes: a Prospective Study

Sakai, Masatoshi; Sasaki, Yasushi; Yoneda, Satoshi; Kimura, Tadashi; Arai, Takashi; Okada, Masatoshi; Hosokawa, Hitoshi; Kato, Kiyoshi; Y, Soeda; Saito, Shigeru

doi:10.1111/j.1600-0897.2004.00145.x

Cited by 49 publications

(40 citation statements)

References 22 publications

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“…15 The concentration of IL-8 used here was relevant physiologically and in the range that was reported recently in vivo in cervical mucus. 16 We previously showed that distension causes an increase in the expression of both PBEF and IL-8 and that PBEF is able to induce the transcription of IL-8. 5 Thus, although PBEF has no effect on chemotaxis, its induction of IL-8 would cause this.…”

Section: Commentmentioning

confidence: 95%

Pre–B-cell colony–enhancing factor is a secreted cytokine-like protein from the human amniotic epithelium

Ognjanovic

Bryant‐Greenwood

2005

American Journal of Obstetrics and Gynecology

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Objective-The purpose of this study was to determine whether pre-B-cell colony-enhancing factor is a secreted cytokine in the human amnion and to study its chemotaxic and antiapoptotic properties.Study design-Pre-B-cell colony-enhancing factor secretion was studied from amniotic epithelial-like WISH cells and primary amniotic epithelial cells that were seeded on squares of immobilon-P membrane and stimulated with lipopolysaccharide or tumor necrosis factor-α , respectively. The pre-B-cell colony-enhancing factor protein was detected both intracellularly and after secretion, as bound to the membrane, by immunostaining and densitometry. Medium and cell lysates that were obtained from WISH cells that were treated with lipopolysaccharide alone or together with a pre-B-cell colony-enhancing factor antisense oligonucleotide to block pre-B-cell colony-enhancing factor translation were also analyzed for secreted pre-B-cell colony-enhancing factor by Western blotting and densitometry. A chemotaxic effect of pre-B-cell colony-enhancing factor on human neutrophils was compared with the chemoattractants interleukin-8 and N-FormylMet-Leu-Phe methyl ester in a rapid fluorescence-based neutrophil migration assay. Apoptosis was induced in primary amniotic epithelial cells and fibroblasts by actinomycin D (1 μg/mL); the antiapoptotic effects of pre-B-cell colony-enhancing factor on early apoptosis were measured by the annexin V assay, and the late effects were determined by measurement of nuclear matrix protein in the media.Results-Treatment of amnion cells that adhered to immobilon-P membrane to induce the secretion of pre-B-cell colony-enhancing factor showed significantly (P < .05) more pre-B-cell colonyenhancing factor protein surrounding the cells compared with the controls. Although the addition of lipopolysaccharide to cultured WISH cells caused the secretion of pre-B-cell colony-enhancing factor into the medium, co-treatment with an antisense oligonucleotide to pre-B-cell colonyenhancing factor obliterated it. Analysis of the cell lysates showed no significant change, which suggests that most of the pre-B-cell colony-enhancing factor protein had been secreted. No significant chemotaxic effects of pre-B-cell colony-enhancing factor were observed; however, pre-B-cell colony-enhancing factor treatment (100 ng/mL), together with actinomycin D, cancelled the early induction of apoptosis, although there was a dose-dependent and significant late antiapoptotic effect on primary amnion epithelial cells (P < .001) and fibroblasts (P < .01).Conclusion-Pre-B-cell colony-enhancing factor is a secreted protein from amniotic epithelial cells. Although it had no chemotaxic effects, it was antiapoptotic for both amniotic epithelial cells and fibroblasts and may protect these cells against apoptosis that is induced by chronic distension, labor, or infection. KeywordsPre-B-cell colony-enhancing factor; Cytokine; Secretion; Chemotaxis; Apoptosis Pre-B-cell colony-enhancing factor (PBEF) was first identified from activated periphera...

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Section: Commentmentioning

confidence: 95%

Pre–B-cell colony–enhancing factor is a secreted cytokine-like protein from the human amniotic epithelium

Ognjanovic

Bryant‐Greenwood

2005

American Journal of Obstetrics and Gynecology

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“…The search for markers of preterm labour has identified increased expression of CXCL8 in cervical mucus, and CCL2 and MIF in maternal serum or amniotic fluid. [120][121][122] The presence of CCL8, CCL5, CCL20, CXCL5 and CCL7 in amniotic fluid is associated with microbial invasion and amniotic cavity inflammation during preterm labour. [123][124][125][126] CXCL11 level is increased in amniotic fluid during second trimester in women who subsequently developed preterm labour.…”

Section: Involvement Of Chemokines In Pregnancy Complicationsmentioning

confidence: 99%

The integrative roles of chemokines at the maternal–fetal interface in early pregnancy

2014

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Embryos express paternal antigens that are foreign to the mother, but the mother provides a special immune milieu at the fetal-maternal interface to permit rather than reject the embryo growth in the uterus until parturition by establishing precise crosstalk between the mother and the fetus. There are unanswered questions in the maintenance of pregnancy, including the poorly understood phenomenon of maternal tolerance to the allogeneic conceptus, and the remarkable biological roles of placental trophoblasts that invade the uterine wall. Chemokines are multifunctional molecules initially described as having a role in leukocyte trafficking and later found to participate in developmental processes such as differentiation and directed migration. It is increasingly evident that the gestational uterine microenvironment is characterized, at least in part, by the differential expression and secretion of chemokines that induce selective trafficking of leukocyte subsets to the maternal-fetal interface and regulate multiple events that are closely associated with normal pregnancy. Here, we review the expression and function of chemokines and their receptors at the maternal-fetal interface, with a special focus on chemokine as a key component in trophoblast invasiveness and placental angiogenesis, recruitment and instruction of immune cells so as to form a fetus-supporting milieu during pregnancy. The chemokine network is also involved in pregnancy complications.

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“…219,240,242 Appendix 5, Table 94 summarises each study's salient features, stratified according to the population of women tested, i.e. asymptomatic antenatal women (two studies) 243,244 and women with symptoms of threatened preterm labour (three studies). 223,232,233 One of the included studies, on the asymptomatic antenatal population, had the test performed two-weekly between 24 and 28 weeks' gestation.…”

Section: Study Characteristics and Qualitymentioning

confidence: 99%

“…223,232,233 One of the included studies, on the asymptomatic antenatal population, had the test performed two-weekly between 24 and 28 weeks' gestation. 244 Except for one study, 223 the studies evaluated IL-8 in cervicovaginal specimens.…”

Section: Study Characteristics and Qualitymentioning

confidence: 99%

Screening to prevent spontaneous preterm birth: systematic reviews of accuracy and effectiveness literature with economic modelling

Honest

Ca²,

Durée³

et al. 2009

Health Technol Assess

185

146

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For primary prevention, an effective, affordable and safe intervention applied to all mothers without preceding testing is likely to be the most cost-effective approach in asymptomatic women in early pregnancy. For secondary prevention among women at risk of preterm labour in later pregnancy, a management strategy based on the results of testing is likely to be more cost-effective. Implementation of a treat-all strategy with simple interventions, such as fish oils, would be premature for asymptomatic women. Universal provision of high-quality ultrasound machines in labour wards is more strongly indicated for predicting spontaneous preterm birth among symptomatic women than direct management, although staffing issues and the feasibility and acceptability to mothers and health providers of such strategies need to be explored. Further research should include investigations of low-cost and effective tests and treatments to reduce and delay spontaneous preterm birth and reduce the risk of perinatal mortality arising from preterm birth.

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Elevated Interleukin‐8 in Cervical Mucus as an Indicator for Treatment to Prevent Premature Birth and Preterm, Pre‐labor Rupture of Membranes: a Prospective Study

Abstract: Successful treatment for women with IL-8 elevations in cervical mucus decreased rates of premature birth or pPROM.

Cited by 49 publications

References 22 publications

Pre–B-cell colony–enhancing factor is a secreted cytokine-like protein from the human amniotic epithelium

Pre–B-cell colony–enhancing factor is a secreted cytokine-like protein from the human amniotic epithelium

The integrative roles of chemokines at the maternal–fetal interface in early pregnancy

Screening to prevent spontaneous preterm birth: systematic reviews of accuracy and effectiveness literature with economic modelling

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