Elevated Innate Peripheral Blood Eosinophilia Fails to Augment Irradiated Cercarial Vaccine-Induced Resistance to Schistosoma mansoni in IL-5 Transgenic Mice

Gl, Freeman; Tominaga, Akira; Takatsu, Kiyoshi; We, Secor; Colley, Daniel G.

doi:10.2307/3284059

Cited by 17 publications

(7 citation statements)

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“…Meanwhile, worm recovery of Mesocestoides corti from IL-5-Tg mice was not signi®cantly dierent from that from nontransgenic mice (Strath et al 1992). Similarly, IL-5-Tg mice vaccinated with irradiated S. mansoni cercariae failed to produce protective immunity to a challenge infection with normal cercariae, as found in C3H/HeN control mice (Freeman et al 1995). More recently, similar ®ndings were obtained from IL-5-Tg mice primarily infected with T. canis or from those vaccinated with excretory and secretory antigens and then challenged with the same parasite (Sugane et al 1996).…”

Section: Discussionsupporting

confidence: 58%

Eosinophilia and intracranial worm recovery in interleukin-5 transgenic and interleukin-5 receptor α chain-knockout mice infected with Angiostrongylus cantonensis

Sugaya

Aoki

Yoshida

et al. 1997

Parasitology Research

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We infected interleukin-5 (IL-5)-transgenic (IL-5-Tg) and IL-5 receptor alpha knockout (IL-5R alpha -/-) mice with Angiostrongylus cantonensis to determine the possible roles of IL-5 and eosinophils in A. cantonensis infection in mice. IL-5-Tg mice demonstrated significantly higher eosinophilia in bone marrow, blood and cerebrospinal fluid (CSF), lower intracranial worm recovery and smaller female worms than naive C3H/HeN mice. Both IL-5-Tg and C3H/HeN mice evoked antigen-specific serum and CSF IgA antibody responses as early as days 5 and 7 postinfection, respectively. Prominent eosinophil infiltration was noted around intracranial worms in the subarachnoid spaces of the mouse brains; eosinophils adhering to the worm surface were degranulated. In contrast, IL-5R alpha -/- mice yielded a higher worm recovery than wild-type or heterozygous mice at day 20 postinfection and failed to provoke CSF eosinophilia. These findings indicate that A. cantonensis infection in the mouse causes IL-5 production and subsequent CSF eosinophilia, the latter probably being involved in the killing of intracranial worms.

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Section: Discussionsupporting

confidence: 58%

Eosinophilia and intracranial worm recovery in interleukin-5 transgenic and interleukin-5 receptor α chain-knockout mice infected with Angiostrongylus cantonensis

Sugaya

Aoki

Yoshida

et al. 1997

Parasitology Research

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“…Nevertheless, only a few transgenic animal models have been used to investigate the involvement of cytokines in immunity to parasites. Considering the helminth S. mansoni , the role of eosinophils as effector cells during infection has been analysed using IL‐5 transgenic mice (30) as has the consequence of IL‐4‐overexpression in the granulomatous reaction (31).…”

Section: Discussionmentioning

confidence: 99%

Cutaneous interleukin‐7 transgenic mice display a propitious environment to Schistosoma mansoni infection

Roye

Delacre

Williams

et al. 2001

Parasite Immunology

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Interleukin (IL)-7 is produced early in Schistosoma mansoni-infected human and murine skin and was recently shown to favour parasite development. In the present work, we investigated the participation of keratinocyte-derived IL-7 in this process. Keratinocytes are the predominant cellular constituents of the epidermis and the first tissue encountered by the parasite when it infects the vertebrate host. We therefore infected IL-7 cutaneous transgenic mice and compared several parasitological and immunological parameters to those of infected littermate controls. In transgenic mice, an increased number of total adult worms was observed while egg number and female fecundity remained unchanged. Additionally, transgenic animals displayed a more intensive hepatic fibrosis. In parallel, infected IL-7 transgenic animals showed a dominant Th2-type humoral response towards egg antigens. The results presented here confirm and reinforce the key role play by IL-7 in S. mansoni-vertebrate host interplay, beginning with keratinocyte-derived IL-7.

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“…25]. However, the role of eosin ophils as an anti-parasite killer cell in vivo remains con troversial [10,11,17,[26][27][28], in spite of the many demon strations that these cells can be potently cytotoxic [1,3,4,8,29], N. brasiliensis has been widely used as a laboratory model in studies of Th2-type immune responses against intestinal helminths. Infection with this parasite is char acterized by high eosinophilia, and experiments using anti-IL-5 antibody demonstrated that IL-5 plays an im portant role in the induction of eosinophilia in mice para sitized with N. brasiliensis [6,7], However, a possible pro tective role of eosinophils in hosts infected with N. brasi liensis has not yet been confirmed.…”

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confidence: 99%

Protective Roles of Eosinophils in <i>Nippostrongylus brasiliensis</i> Infection

Shin¹,

Osada²,

Chai

et al. 1997

Int Arch Allergy Immunol

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Nippostrongylus brasiliensis infection is characterized by blood and tissue eosinophilia induced by interleukin (IL)-5 secreted from CD4+ T cells. However, it is still obscure whether eosinophils play an important role in the protection against N. brasiliensis infection. In this study we attempted to determine whether the in vivo environment of IL-5 transgenic mice, characterized by high eosinophil production, could affect the worm burden after N. brasiliensis infection. Kinetic studies on the infection demonstrated a significantly lower worm recovery from the intestine of IL-5 transgenic mice compared to age-matched background controls. This tendency was also observed at the lung stage of the infection. Furthermore, with respect to elevation of the serum IgE concentration, the peak level was observed at 2 weeks after infection in infected background control mice with four times higher concentrations than those of uninfected mice. In contrast, the increase of IgE concentration in IL-5 transgenic mice was very limited and low. The adoptive transfer of eosinophils from IL-5 transgenic mice into background control animals resulted in the reduction of worm recovery from the lungs, suggesting that eosinophils play a key role in the protection against migrating larvae of N. brasiliensis. These results indicate that the innate high level of eosinophils due to constitutive production of IL-5 augments immunity against N. brasiliensis infection.

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Elevated Innate Peripheral Blood Eosinophilia Fails to Augment Irradiated Cercarial Vaccine-Induced Resistance to Schistosoma mansoni in IL-5 Transgenic Mice

Cited by 17 publications

References 0 publications

Eosinophilia and intracranial worm recovery in interleukin-5 transgenic and interleukin-5 receptor α chain-knockout mice infected with Angiostrongylus cantonensis

Eosinophilia and intracranial worm recovery in interleukin-5 transgenic and interleukin-5 receptor α chain-knockout mice infected with Angiostrongylus cantonensis

Cutaneous interleukin‐7 transgenic mice display a propitious environment to Schistosoma mansoni infection

Protective Roles of Eosinophils in <i>Nippostrongylus brasiliensis</i> Infection

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