Elevated immune response in Octopus rubescens under ocean acidification and warming conditions

Culler-Juarez, Monica  E.; Onthank, Kirt L.

doi:10.1007/s00227-021-03913-z

Cited by 9 publications

(7 citation statements)

References 47 publications

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“…Elevated CO 2 alters the molluscan immune response, with most research focusing on bivalves (Bibby et al, 2008;Liet al, 2015;Liu et al, 2016;Su et al, 2018;Wu et al, 2016)), though the immune response of an octopus was also affected by elevated CO 2 (Culler-Juarez & Onthank, 2021). Here, we found DE of genes that regulate immune signal transduction pathways and which are also implicated in the molluscan immune response (map4k5/3, syvn1-b, psenen , cbs) (Canesi et al, 2006;De Zoysa et al, 2010;Goodson et al, 2005;Salazar et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic responses in the nervous system and correlated behavioural changes of a cephalopod exposed to ocean acidification

Thomas,

Huerlimann,

Schunter

et al. 2023

Preprint

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The nervous system is central to coordinating behavioural responses to environmental change, likely including ocean acidification (OA). However, a clear understanding of neurobiological responses to OA is lacking, especially for marine invertebrates. We evaluated the transcriptomic response of the central nervous system (CNS) and eyes of the two-toned pygmy squid (Idiosepius pygmaeus) to OA conditions, using a de novo transcriptome assembly created with long read PacBio ISO-sequencing data. We then correlated patterns of gene expression with CO treatment levels and OA-affected behaviours in the same individuals. OA induced transcriptomic responses within the nervous system related to various different types of neurotransmission, neuroplasticity, immune function and oxidative stress. These molecular changes may contribute to OA-induced behavioural changes, as suggested by correlations between gene expression profiles, CO treatment and OA-affected behaviours. This study provides the first molecular insights into the neurobiological effects of OA on a cephalopod and correlates molecular changes with whole animal behavioural responses, helping to bridge the gap in our knowledge between environmental change and animal responses.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic responses in the nervous system and correlated behavioural changes of a cephalopod exposed to ocean acidification

Thomas,

Huerlimann,

Schunter

et al. 2023

Preprint

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“…The temperature of the aquaria was not controlled and allowed to match the room temperature. Independent measurements of pH were taken of each aquarium daily using the m-cresol purple spectrophotometric method ( Dickson et al, 2007 ) as modified by Culler-Juarez and Onthank (2021) . Total pH values were calculated from absorbance values in R using the “specpH” function found the in OTools package version 22.08.01 ( https://github.com/KirtOnthank/Otools ).…”

Section: Methodsmentioning

confidence: 99%

Synergistic and antagonistic interactions of oxybenzone and ocean acidification: new insight into vulnerable cellular processes in non-calcifying anthozoans

Morgan,

Williams,

Breeze

et al. 2024

Front. Physiol.

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Cnidarians face significant threats from ocean acidification (OA) and anthropogenic pollutants such as oxybenzone (BP-3). The convergence of threats from multiple stressors is an important area to investigate because of potential significant synergistic or antagonistic interactions. Real-time quantitative PCR was performed to characterize the expression profiles of twenty-two genes of interest (GOI) in sea anemones (Exaiptasia diaphana) exposed to one of four treatments: 1) 96 h of OA conditions followed by a 4 h exposure to 20 ppb BP-3; 2) Exposure to 4 h 20 ppb BP-3 without 96 h of OA; 3) Exposure to 96 h of OA alone; or 4) laboratory conditions with no exposure to BP-3 and/or OA. These 22 GOIs represent cellular processes associated with proton-dependent transport, sodium-dependent transport, metal cation binding/transport, extracellular matrix, amino acid metabolism/transport, immunity, and/or steroidogenesis. These 22 GOIs provide new insight into vulnerable cellular processes in non-calcifying anthozoans exposed to OA and BP-3. Expression profiles were categorized as synergistic, antagonistic, or additive of BP-3 in the presence of OA. Two GOIs were synergistic. Fifteen GOIs were antagonistic and the remaining five GOIs were additive in response to BP-3 in acidified seawater. A subset of these GOIs appear to be candidate biomarkers for future in situ investigations. In human health, proton-dependent monocarboxylate transporters (MCTs) are promising pharmacological targets and recognized as potential biomarkers. By comparison, these same MCTs appear to be targets of xenobiotic chemical pollutants in cnidarian physiology. In the presence of BP-3, a network of collagen synthesis genes are upregulated and antagonistic in their expression profiles. Cytochrome b561 is a critical protein required for collagen synthesis and in silico modeling demonstrates BP-3 binds in the pocket of cytochrome b561. Understanding the underlying molecular mechanisms of “drug-like” compounds such as BP-3 may lead to a more comprehensive interpretation of transcriptional expression profiles. The collective antagonistic responses of GOIs associated with collagen synthesis strongly suggests these GOIs should be considered candidate biomarkers of effect. GOIs with synergistic and additive responses represent candidate biomarkers of exposure. Results show the effects of OA and BP-3 are interactive with respect to their impact on cnidarians. This investigation offers mechanistic data that supports the expression profiles and underpins higher order physiological responses.

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“…After being held in aquaria for at least 1 day, octopuses were transferred to treatment tanks as previously described ( Culler-Juarez and Onthank, 2021 ). Each tank (113.5 L) was made using an insulated cooler with an overhead window in the lid to allow observation of octopus.…”

Section: Methodsmentioning

confidence: 99%

Bathyal octopus, Muusoctopus leioderma, living in a world of acid: First recordings of routine metabolic rate and critical oxygen partial pressures of a deep water species under elevated pCO2

et al. 2022

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Elevated atmospheric CO2 as a result of human activity is dissolving into the world’s oceans, driving a drop in pH, and making them more acidic. Here we present the first data on the impacts of ocean acidification on a bathyal species of octopus Muusoctopus leioderma. A recent discovery of a shallow living population in the Salish Sea, Washington United States allowed collection via SCUBA and maintenance in the lab. We exposed individual Muusoctopus leioderma to elevated CO2 pressure (pCO2) for 1 day and 7 days, measuring their routine metabolic rate (RMR), critical partial pressure (Pcrit), and oxygen supply capacity (α). At the time of this writing, we believe this is the first aerobic metabolic data recorded for a member of Muusoctopus. Our results showed that there was no change in either RMR, Pcrit or α at 1800 µatm compared to the 1,000 µatm of the habitat where this population was collected. The ability to maintain aerobic physiology at these relatively high levels is discussed and considered against phylogeny and life history.

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Elevated immune response in Octopus rubescens under ocean acidification and warming conditions

Cited by 9 publications

References 47 publications

Transcriptomic responses in the nervous system and correlated behavioural changes of a cephalopod exposed to ocean acidification

Transcriptomic responses in the nervous system and correlated behavioural changes of a cephalopod exposed to ocean acidification

Synergistic and antagonistic interactions of oxybenzone and ocean acidification: new insight into vulnerable cellular processes in non-calcifying anthozoans

Bathyal octopus, Muusoctopus leioderma, living in a world of acid: First recordings of routine metabolic rate and critical oxygen partial pressures of a deep water species under elevated pCO2

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