Elevated IL‐6 in Midtrimester Amniotic Fluid Is Involved with the Onset of Preeclampsia

Nakabayashi, Masao; Sakura, Mari; Takeda, Yoshihiko; Sato, Kana

doi:10.1111/j.1600-0897.1998.tb00526.x

Cited by 28 publications

(23 citation statements)

References 23 publications

(1 reference statement)

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“…These results are consistent with reports of increased IL-8 production in trophoblasts (Bowen et al, 2005) and elevated IL-8 levels in maternal and umbilical cord serum as well as amniotic fluid of preeclamptic women (Nakabayashi et al, 1998;Laskowska et al, 2007). In contrast, Wang et al (1999) found a decrease in placental IL-8 production in preeclampsia.…”

Section: Transporter Expression and Pregnancy Conditionsupporting

confidence: 92%

ATP-Binding Cassette Transporter Expression in Human Placenta as a Function of Pregnancy Condition

Mason

Buhimschi²,

Buhimschi³

et al. 2011

Drug Metab Dispos

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ABSTRACT:Fetal drug exposure is determined by the type and concentration of placental transporters, and their regulation is central to the development of new treatments and delivery strategies for pregnant women and their fetuses. We tested the expression of several clinically important transporters in the human placenta associated with various pregnancy conditions (i.e., labor, preeclampsia, and preterm labor-inflammation). Placentas were obtained from five groups of women at the time of primary cesarean section: 1) term no labor; 2) term labor; 3) preterm no labor (delivered for severe preeclampsia); 4) preterm labor without inflammation (PTLNI); and 5) preterm labor with inflammation (PTLI). Samples were analyzed by Western blot and immunohistochemistry to identify changes in protein expression. Relative mRNA expression was determined by quantitative real-time polymerase chain reaction. A functional genomic approach was used to identify placental gene expression and elucidate molecular events that underlie the given condition. Placental expression of ATP-binding cassette transporters from women in labor and women with preeclampsia was unaltered. Multidrug resistance protein 1 (MDR1) and breast cancer resistance protein (BCRP) and mRNA expression increased in placentas of women with preterm labor with inflammation. Molecular pathways of genes up-regulated in PTLI samples included cytokinecytokine receptor interactions and inflammatory response compared with those in the PTLNI group. The mRNA expression of MDR1 and BCRP was correlated with that of interleukin-8, which also increased significantly in PTLI samples. These data suggest that the transfer of drugs across the placenta may be altered in preterm pregnancy conditions associated with inflammation through changes in MDR1 and BCRP.

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Section: Transporter Expression and Pregnancy Conditionsupporting

confidence: 92%

ATP-Binding Cassette Transporter Expression in Human Placenta as a Function of Pregnancy Condition

Mason

Buhimschi²,

Buhimschi³

et al. 2011

Drug Metab Dispos

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“…This corroborates the report that significantly elevated levels of IL-6 and IL-8 were found in mid-trimester amniotic fluid of the future preeclamptic group compared with the normal pregnancies (19).…”

Section: Discussionsupporting

confidence: 81%

Aberrations in Placental Cytokine mRNA Related to Intrauterine Growth Retardation

et al. 2002

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“…Previous reports suggested that human placenta syncytiotrophoblast could produce IL-6, and IL-6 acts as a pleiotropic protein in stimulating hematopoiesis (Caracciolo et al, 1989;Kameda et al, 1990). However, substantially enhanced IL-6 has been reported to be associated with the pathophysiology of intrauterine infection or infection-induced preterm labor and delivery or pre-eclampsia (Nakabayashi et al, 1998;Hsu et al, 2000;Figueroa et al, 2005;Suzuki et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Immunological aspects of human amniotic fluid cells: Implication for normal pregnancy

Yan

Lin

Chen

et al. 2008

Cell Biology International

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Amniotic fluid cells (AFCs) are routinely obtained and expanded in vitro for prenatal diagnosis; nevertheless current knowledge about their properties is limited. The detailed mechanisms underlying normal pregnancies are yet to be discovered. The goal of this study was to identify the immunological aspects of AFCs including cytokine production and human leukocyte antigen (HLA) expression, and to discuss its implication for pregnancy. Eighty-six samples of AFCs were determined for HLA expression before and after culture. Cytokine production was measured with flow cytometry in AFC culture supernatants. Treatment of interferon (IFN)-gamma on induction of HLA-DR expression in cultured AFCs was also investigated. Data indicated that both fresh and cultured AFCs express HLA-I, HLA-G, but not HLA-DR, and the cultured AFCs predominately produce the cytokine interleukin (IL)-6. Importantly, we observed that IFN-gamma could induce HLA-DR expression on cultured AFCs in a dose-dependent manner. Taken together, our results indicated that AFCs are functionally active cells and are significant in pregnancy.

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Elevated IL‐6 in Midtrimester Amniotic Fluid Is Involved with the Onset of Preeclampsia

Abstract: Elevated cytokines in midtrimester amniotic fluid suggest an abnormal fetomaternal immune response occurring before the clinical manifestation of preeclampsia. Cytokine-induced suppression of thrombomodulin in trophoblasts may be directly involved in the pathogenesis of preeclampsia.

Cited by 28 publications

References 23 publications

ATP-Binding Cassette Transporter Expression in Human Placenta as a Function of Pregnancy Condition

ATP-Binding Cassette Transporter Expression in Human Placenta as a Function of Pregnancy Condition

Aberrations in Placental Cytokine mRNA Related to Intrauterine Growth Retardation

Immunological aspects of human amniotic fluid cells: Implication for normal pregnancy

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