Elevated hCG and a high proportion of hCGβ in serum preceding the diagnosis of trophoblastic disease by seven months

Vartiainen, Juhani; Alfthan, Henrik; Lehtovirta, Pentti; Stenman, Ulf‐Hǎkan

doi:10.1111/j.1471-0528.2002.00195.x

Cited by 18 publications

(2 citation statements)

References 11 publications

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“…In retrospect, the application of multiply charged redox cations (e.g., [Ru(NH 3 ) 6 ] 3+ ) for the quantitation of DNA probes on electrode surfaces has been developed as a routine practice [ 29 , 30 ]. The validity of this method relies on the following assumptions: (1) the electrostatic interaction is the only binding force between redox cations and DNA strands; (2) the determination of the amount of trapped redox cations is accurate; and (3) other cations such as Na + and K + adsorbed onto DNA phosphate backbones can be completely replaced by [Ru(NH 3 ) 6 ] 3+ , which means the charge compensation of the DNA phosphate backbones is exclusively provided by redox cations [ 26 ]. Because of the high binding affinity to the DNA monolayer, a low concentration of [Ru(NH 3 ) 6 ] 3+ (<5.0 μM) is suitable for ensuring that the saturation of the DNA-modified surface with redox-active cations produces negligible background current (of the diffused species) [ 31 , 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…In human fluids, hCG is present in many forms such as hCGα subunit, hCGβ subunit, intact hCG, and hCG β fragment. Beyond the early pregnancy tests (done qualitatively), the quantitative determination of the hCG amount in urine can be used to monitor abnormal pregnancy conditions and to diagnose other complications such as testicular cancer and gestational trophoblastic diseases [ 24 , 25 , 26 ]. The conventional quantitative hCG detection method is ELISA, which uses a 96-well microtiter plate for preparing the assay and a spectrophotometer-type plate reader to read the signal.…”

Section: Introductionmentioning

confidence: 99%

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DNA-Redox Cation Interaction Improves the Sensitivity of an Electrochemical Immunosensor for Protein Detection

et al. 2015

Sensors

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A simple DNA-redox cation interaction enhancement strategy has been developed to improve the sensitivity of electrochemical immunosensors for protein detection. Instead of labeling with fluorophores or redox-active groups, the detection antibodies were tethered with DNA single strands. Based on the electrostatic interaction between redox cations ([Ru(NH3)6]3+) and negatively charged DNA backbone, enhanced electrochemical signals were obtained. Human chorionic gonadotropin (hCG) detection has been performed as a trial analysis. A linear response range up to the concentration of 25 mIU/mL and a detection limit of 1.25 mIU/mL have been achieved, both are comparable with the ultrasensitive enzyme-linked immunosorbent assay (ELISA) tests. The method also shows great selectivity towards hCG over other hormones such as thyroid stimulating hormone (TSH) and follicle stimulating hormone (FSH). By and large, our approach bears the merits of cost effectiveness and simplicity of instrumentation in comparison with conventional optical detection methods.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

DNA-Redox Cation Interaction Improves the Sensitivity of an Electrochemical Immunosensor for Protein Detection

et al. 2015

Sensors

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A Simple, Selective and Sensitive Immunoassay for Determination of Human Chorionic Gonadotrophin Based on Chemiluminescence Resonance Energy Transfer

Lei

Mei

Zhou

et al. 2014

J Chinese Chemical Soc

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A simple, selective and sensitive immunoassay was developed for the determination of human chorionic gonadotrophin (HCG) based on the chemiluminescence resonance energy transfer (CRET). Two kinds of antibodies were used to form a sandwich-type immunocomplex to ensure the selectivity of immunoassay. Based on the simple magnetic separation, amplification feature of gold nanoparticles and CRET phenomenon, this method could be used for highly sensitive determination of HCG without tedious washing steps. Under the optimized conditions, a linear range was obtained when the concentrations of HCG were changed from 0.15 to 5 mIU mL -1 (R = 0.992). This immunoassay could be used for the determination of HCG in serum samples, which provided a promising potential in clinical diagnosis.

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Electrochemical immunoassay for human chorionic gonadotrophin based on Pt hollow nanospheres and silver/titanium dioxide nanocomposite matrix

Yang

Yuan

Zhuo

et al. 2010

J of Chemical Tech & Biotech

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BACKGROUND: A new electrochemical immunosensor for human chorionic gonadotropin (HCG) assay was developed based on Pt hollow nanospheres and silver/titanium dioxide nanocomposites. Silver nanoparticles were initially doped into TiO 2 and chitosan (Cs) colloids to form silver/titanium dioxide nanocomposites (Ag-TiO 2 -Cs), which had good redox electrochemical activity and excellent film-forming ability. Then, the free amino groups of chitosan were used to attach Pt hollow nanospheres for immobilization of human chorionic gonadotropin antibody (anti-HCG) to construct an immunosensor.

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Elevated hCG and a high proportion of hCGβ in serum preceding the diagnosis of trophoblastic disease by seven months

Cited by 18 publications

References 11 publications

DNA-Redox Cation Interaction Improves the Sensitivity of an Electrochemical Immunosensor for Protein Detection

DNA-Redox Cation Interaction Improves the Sensitivity of an Electrochemical Immunosensor for Protein Detection

A Simple, Selective and Sensitive Immunoassay for Determination of Human Chorionic Gonadotrophin Based on Chemiluminescence Resonance Energy Transfer

Electrochemical immunoassay for human chorionic gonadotrophin based on Pt hollow nanospheres and silver/titanium dioxide nanocomposite matrix

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