Elevated faecal ovotransferrin concentrations are indicative for intestinal barrier failure in broiler chickens

Goossens, Evy; Debyser, Griet; Callens, Chana; Gussem, M. de; Dedeurwaerder, Annelike; Devreese, Bart; Haesebrouck, Freddy; Flügel, Monika; Pelzer, Stefan; Thiemann, Frank; Ducatelle, Richard; Immerseel, Filip Van

doi:10.1186/s13567-018-0548-4

Cited by 23 publications

(41 citation statements)

References 33 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result suggests a possible loss of intestinal integrity as a result of feeding high levels of rye and consequences on systemic inflammation. The elevated ovotransferrin in faecal material has recently been shown in response to necrotic enteritis [10] making it a worthwhile candidate for future validation studies. It is noted that activity of ovotransferrin is decreased by increasing the temperature [10].…”

Section: Plos Onementioning

confidence: 99%

“…Intestinal fatty acid binding proteins is cytoplasmic protein exclusive to the intestinal enterocytes and can be regarded as a biomarker [8]. Other potential biomarkers include lipocalin 2 [9], ovotransferrin [10], superoxide dismutase [11], fibronectin [4] and intestinal alkaline phosphatase [12]. In most cases, it is unknown how useful excreta biomarkers can be in poultry and even the reference values are lacking.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens

et al. 2020

View full text Add to dashboard Cite

Increased intestinal permeability (IP) and inflammation are both linked with functionality of the intestinal barrier and in particular enterocytes. Currently, almost all assessment methods of the intestinal barrier function are invasive. The present study aimed to quantify selected proteins as novel biomarkers in excreta of broiler chickens to facilitate non-invasive assessment of gut barrier function using enzyme-linked immunosorbent assays (ELISA). It was further hypothesised that probiotics as feed additives may counteract gut barrier dysfunction. A 3 × 2 factorial arrangement of treatments was used with the main factors being gut barrier dysfunction models (control, rye-based diet, and dexamethasone-DEX) with and without probiotic supplementation (a three-strain Bacillus) using 72 male Ross 308 day-old chickens. Each of the 6 experimental treatments was replicated 12 times. On d 21 of age, fluorescein isothiocyanate dextran (FITC-d) uptake into serum was examined to test IP. Fresh excreta samples were collected on d 20. The biomarkers included alpha-1 antitrypsin (A1AT), intestinal fatty acid binding protein (I-FABP), lipocalin-2 (LCN2), fibronectin (FN), intestinal alkaline phosphatase (IAP), ovotransferrin (OVT) and superoxide dismutase [Cu-Zn] (SOD1). Only DEX increased (P<0.001) FITC-d passage to the blood on d 21 of age, indicating a greater IP. The excreta concentrations of A1AT, I-FABP and SOD1 were unaltered by the experimental treatments. DEX increased (P<0.05) FN concentration in excreta compared with control birds. Conversely, inclusion of rye in the diet reduced (P<0.05) FN but increased (P<0.001) OVT in excreta. Independently, DEX decreased IAP (P<0.05) in excreta compared with control and rye-fed birds. The excreta concentration of LCN2 tended (P = 0.086) to increase in birds injected by DEX. There was no demonstrable effect of probiotic addition on any of the studied parameters. Among the tested biomarkers, FN, IAP, and LCN2 revealed promise as biomarkers of intestinal barrier function quantified by ELISA kits.

show abstract

Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Until now, few seem to be ready for practical use in the field. Recently, ovotransferrin has been proposed as fecal biomarker for intestinal leakage caused by coccidiosis and necrotic enteritis in broilers [22].…”

Section: Introductionmentioning

confidence: 99%

Host intestinal biomarker identification in a gut leakage model in broilers

Meyer

Eeckhaut

Ducatelle

et al. 2019

Vet Res

Self Cite

View full text Add to dashboard Cite

Intestinal health problems are a major issue in the poultry industry. Quantifiable easy-to-measure biomarkers for intestinal health would be of great value to monitor subclinical intestinal entities that cause performance problems and to evaluate control methods for intestinal health. The aim of the study was to identify host protein biomarkers for intestinal inflammation and intestinal barrier damage. Proteomic analysis was conducted on ileal and colonic content samples of broilers under an experimental gut damage and inflammation model. Effects of the challenge treatment resulted in a worse gut condition based on macroscopic gut appearance ( p < 0.0001). Also microscopic changes such as shortening of the villi and increased crypt depth ( p < 0.0001) as well as higher infiltration of T-lymphocytes ( p < 0.0001) were seen in the duodenal tissue of challenged animals. Several candidate proteins associated with inflammation, serum leakage and/or tissue damage were identified with an increased abundance in intestinal content of challenged animals ( p < 0.05). Conversely, brush border enzymes were less abundant in intestinal content of challenged animals ( p < 0.05). These candidate biomarkers have potential to be used in the field for detection of gut barrier failure in broilers.

show abstract

“…The reduced growth rate observed in diseased birds might be explained by a reduced intestinal barrier function and inflammation in broilers which develop NE. Indeed, our research group has recently shown that broilers with severe necrotic enteritis suffer from intestinal barrier failure, whereas no intestinal leakage was measured in birds that did not develop disease or showed mild NE (Goossens et al, 2018). This is in accordance with the current study where, despite the large effect of C. perfringens challenge on both BW and BWG for birds that developed moderate to severe NE, no differences could be observed between birds without lesions and birds that developed only mild necrotic lesions.…”

Section: Discussionmentioning

confidence: 99%

Rapid growth predisposes broilers to necrotic enteritis

et al. 2019

View full text Add to dashboard Cite

Over the past 50 years, intentional genetic selection within the broiler industry has led to major improvements in both body weight gain (BWG) and feed conversion efficiency. Next to its economic advantages, enhancing BWG can increase the risk for metabolic and skeletal disorders. The aim of this study was to examine whether higher BWG is a predisposing factor for broiler necrotic enteritis. In this study, 300 broilers were challenged with Clostridium perfringens using a well-established, previously described challenge model. It was found that birds with a higher body weight (BW) and BWG before challenge were predisposed to develop more severe necrotic enteritis lesions. After challenge, the average BWG of the birds developing mild to severe lesions dropped significantly, negatively affecting animal welfare and performance. These results show a significant interplay between BWG and the development of necrotic enteritis lesions. This raises the question whether there is a limit to broiler performance with respect to maintaining intestinal health, and whether decreasing BWG (at certain stages of the growth cycle) can be part of a plan to prevent intestinal pathology. Research highlights Higher body weight is a predisposing factor to necrotic enteritis in broilers.

show abstract

Elevated faecal ovotransferrin concentrations are indicative for intestinal barrier failure in broiler chickens

Cited by 23 publications

References 33 publications

Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens

Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens

Host intestinal biomarker identification in a gut leakage model in broilers

Rapid growth predisposes broilers to necrotic enteritis

Contact Info

Product

Resources

About