Elevated expression patterns and tight correlation of the PLCE1 and NF-κB signaling in Kazakh patients with esophageal carcinoma

Abstract

This study investigated the expression of the phospholipase C epsilon 1 (PLCE1) and nuclear factor-kappaB (NF-κB)-related proteins in Kazakh patients with esophageal squamous cell carcinoma (ESCC). Tissue microarrays of 90 ethnic Kazakh patients with ESCC and exhibiting clinical characteristics were analyzed for protein expression of PLCE1, IKKβ, IKBα, p50, and p65 by immunohistochemistry. Correlations between histoscores of PLCE1 and NF-κB-related proteins were determined using Spearman's rank correlation tests. Expression of PLCE1 and NF-κB-related proteins significantly increased in tumor tissues compared with normal esophageal tissues (P = 9.48 × 10(-7), 1.24 × 10(-5), 0.004, 0.003, and 2.83 × 10(-5), respectively). Upregulation of PLCE1 was significantly correlated with advanced tumor-node-metastasis stages (P = 0.018) and lymph node metastasis (P = 0.003). Overexpression of IKKβ and IKBα was associated with ESCC stages I/II (P = 3.36 × 10(-4) and 0.022, respectively). Increased expression of p50 was significantly higher in patients with lymph node metastasis than without lymph node metastasis (P = 0.048). Elevated expression of p65 protein was significantly correlated with poor and moderately differentiated ESCC and depth of tumor invasion (P = 0.026 and 0.010, respectively). Significant positive correlations were observed between the expression of PLCE1 and NF-κB-related proteins, especially IKKβ (r = 0.246 and P = 0.025) and p50 (r = 0.244 and P = 0.024). These results suggest, for the first time, that upregulation of PLCE1 is correlated with increased expression of NF-κB-related proteins in Kazakh patients with ESCC, suggesting that interaction between PLCE1 with the NF-κB signal pathway may be responsible for the carcinogenesis of ESCC, such as ESCC-related inflammation.

Prognostic Value of PLCE1 Expression in Upper Gastrointestinal Cancer: a Systematic Review and Meta-analysis

“…Our study also showed that no significant correlation between PLCE1 and clinicopathological parameters, such as invasion depth, histologic grade, and lymph node metastasis in esophageal cancer. However, PLCE1 expression was associated with tumor progression in two Kazakh groups in the study of Chen and Cui et al (2013;2014b), but not in another Kazakh group in the study of Zhu et al (2012). This result suggests that ethnicity population heterogeneity may influence gene expression.…”

A Genetic Variant in miRNA-219-1 Is Associated with Risk of Esophageal Squamous Cell Carcinoma in Chinese Kazakhs

“…Carcinogenesis and development of ESCC are complex biological processes involving multiple factors, including environmental risk factors. ESCC is a polygenic hereditary disease that can also be influenced by genetic factors [ 5 , 14 , 30 , 31 ]. Along with other researchers, we have long carried on the etiological and epidemiological investigation of numerous aspects of the high Kazakh ESCC incidence rate, which may include traditional habits and customs, such as eating hot meals, hard foods, yogurt knots, and tea; smoking; lack of folic acid; natural geographical environment [ 31 ]; genetic factors, such as PLCE1, hRFT1, and ECRG2 [ 14 , 32 , 33 ]; and microbial factors, such as HPV [ 34 ].…”

PLCE1 polymorphisms and expression combined with serum AFP level predicts survival of HBV-related hepatocellular carcinoma patients after hepatectomy

“…Gene knockdown studies in ESCC cell lines suggested that PLCE1 has an oncogenic function in ESCC [49, 50]. In addition, PLCE1 expression was positively correlated with that of nuclear factor κB-related proteins in Kazakh ESCC patients [51], and negatively correlated with TP53 in HCC and ESCC cells and lung cancer [32, 52, 53]. Distinct microRNAs were shown to suppress PLCE1 expression and thereby affect cancer development and patient prognosis [44, 49, 54].…”