Elevated expression of stemness genes in adipose-derived mesenchymal stem cells cultured on fibrin scaffold

Mousavifard, Arezoo; Najafabadi, Elahe Pourazizi; Rahnama, Mahshid Akhavan; Anbarlou, Azadeh; Atashi, Amir

doi:10.1007/s12038-020-00050-5

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…Our results showed that HWJSC cultured in FIBRIAGAR-3D significantly overexpressed CD105 at all concentrations, and NANOG and OCT4 at the highest concentration, as compared to cells cultured using 2D methods, and kept the expression of CD73, CD90 and SOX2. These results are in agreement with previous reports demonstrating that 3D culturing is able to enhance pluripotency and differentiation capability of HWJSC and other types of MSC as compared to 2D methods ( Hess et al, 2017 ; Mousavifard et al, 2020 ; Thakur et al, 2022 ). The fact that FIBRIAGAR-3D succeeded in improving these markers on cultured HWJSC could contribute to obtaining cell populations with increased differentiation potential and, therefore, with expanded clinical applications.…”

Section: Discussionsupporting

confidence: 93%

“…Although these systems allow cell expansion, development of more efficient culture protocols providing higher cell expansion efficiency is needed. In fact, it has been demonstrated that MSC tend to diminish their proliferation potential and their differentiation capability when cultured on 2D systems ( Yan et al, 2014 ; McKee and Chaudhry, 2017 ), with a significant reduction in the expression of some of the typical markers of pluripotency expressed by MSC ( Hess et al, 2017 ; Mousavifard et al, 2020 ; Thakur et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…In the human tissue, cell adhesion, proliferation, survival and differentiation are directly controlled and regulated by three-dimensional (3D) cell-ECM interactions mediated by transmembrane cell receptors and ECM integrins ( Brizzi et al, 2012 ). The lack of these important clues and interactions in traditional 2D culture methods could contribute to explain why MSC cultured in culture plates tend to lose proliferation and differentiation potential ( Mousavifard et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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A novel 3D biofabrication strategy to improve cell proliferation and differentiation of human Wharton’s jelly mesenchymal stromal cells for cell therapy and tissue engineering

Blanco-Elices,

Oruezabal,

Sánchez-Porras

et al. 2023

Front. Bioeng. Biotechnol.

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Purpose: Obtaining sufficient numbers of cells in a short time is a major goal of cell culturing in cell therapy and tissue engineering. However, current bidimensional (2D) culture methods are associated to several limitations, including low efficiency and the loss of key cell differentiation markers on cultured cells.Methods: In the present work, we have designed a novel biofabrication method based on a three-dimensional (3D) culture system (FIBRIAGAR-3D). Human Wharton’s jelly mesenchymal stromal cells (HWJSC) were cultured in 3D using 100%, 75%, 50%, and 25% concentrations of fibrin-agarose biomaterials (FA100, FA75, FA50 and FA25 group) and compared with control cells cultured using classical 2D systems (CTR-2D).Results: Our results showed a significant increase in the number of cells generated after 7 days of culture, with cells displaying numerous expansions towards the biomaterial, and a significant overexpression of the cell proliferation marker KI67 was found for the FA75 and FA100 groups. TUNEL and qRT-PCR analyses demonstrated that the use of FIBRIAGAR-3D was not associated with an induction of apoptosis by cultured cells. Instead, the 3D system retained the expression of typical phenotypic markers of HWJSC, including CD73, CD90, CD105, NANOG and OCT4, and biosynthesis markers such as types-I and IV collagens, with significant increase of some of these markers, especially in the FA100 group. Finally, our analysis of 8 cell signaling molecules revealed a significant decrease of GM-CSF, IFN-g, IL2, IL4, IL6, IL8, and TNFα, suggesting that the 3D culture system did not induce the expression of pro-inflammatory molecules.Conclusion: These results confirm the usefulness of FIBRIAGAR-3D culture systems to increase cell proliferation without altering cell phenotype of immunogenicity and opens the door to the possibility of using this novel biofabrication method in cell therapy and tissue engineering of the human cornea, oral mucosa, skin, urethra, among other structures.

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Section: Discussionsupporting

confidence: 93%