Elevated expression levels of the 14-3-3 family of proteins in lung cancer tissues

Nakanishi, Kozo; Hashizume, Shuichi; Kato, Masaaki; Honjoh, Tsutomu; Setoguchi, Yuko; Yasumoto, Kosei

doi:10.3233/hab-1997-8404

Cited by 53 publications

(38 citation statements)

References 14 publications

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“…These data were consistent with previous study that indicated 14-3-3 family of proteins were elevated in human lung cancer tissues. 15 For comparison we also examined three cultured non-small cell lung carcinoma cell lines, A549, H358 and H322. Similar to the data obtained from biopsies, 14-3-3␤, ␥ (except in H322), , and , in addition to 14-3-3⑀ and , were present in abundance (Fig.…”

Section: Different 14-3-3 Isoforms Exist In Normal and Cancerous Tissuesmentioning

confidence: 99%

“…14 Lung cancer is the number one cancer killer in the United States and a previous report indicates that 14-3-3 proteins are highly expressed in lung cancers and that this expression is only restricted to the cancer tissues. 15 This suggests that 14-3-3 proteins may play an important role in lung tumorigenesis. There is at present, however, no information on whether the elevated levels of 14-3-3 protein are due to a specific isoform(s) in lung cancers.…”

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confidence: 99%

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Isoform‐specific expression of 14‐3‐3 proteins in human lung cancer tissues

Liu

Qiao

et al. 2004

Intl Journal of Cancer

158

137

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14-3-3 Proteins play important roles in a wide range of vital regulatory processes, including signal transduction, apoptosis, cell cycle progression and DNA replication. In mammalian cells, 7 14-3-3 isoforms (␤, ␥, ⑀, , , and ) have been identified and each of these seems to have distinct tissue localizations and isoform-specific functions. Previous studies have shown that 14-3-3 protein levels are higher in human lung cancers as compared to normal tissues. It is unclear, however, which of the 14-3-3 isoform(s) are overexpressed in these cancers. In our study, the levels of all seven 14-3-3 isoforms were examined by RT-PCR and Western blotting. We show that the message for only two isoforms, 14-3-3⑀ and , could be detected in normal tissues. In lung cancer biopsies, however, four isoforms, 14-3-3␤, ␥, , and , in addition to 14-3-3⑀ and , were present in abundance. The expression frequency of 14-3-3␤, ␥, and isoforms was 11, 10, 13 and 8 of the 14 biopsies examined, respectively. The data from immunohistochemical staining and Western blotting were consistent with the RT-PCR results. Given the prevalence of elevated 14-3-3 expression in human lung cancers we propose that these proteins may be involved in lung cancer tumorigenesis and that specific 14-3-3 proteins may be useful as markers for lung cancer diagnosis and targets for therapy.

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Section: Different 14-3-3 Isoforms Exist In Normal and Cancerous Tissuesmentioning

confidence: 99%

mentioning

confidence: 99%

Isoform‐specific expression of 14‐3‐3 proteins in human lung cancer tissues

Liu

Qiao

et al. 2004

Intl Journal of Cancer

158

137

View full text Add to dashboard Cite

show abstract

“…enzyme and substrate) together, and to stabilize the conformation of the associated proteins. Expression of the 14-3-3 was increased signi®cantly in lung cancer tissues compared with the neighboring normal part of lung (Nakanishi et al, 1997) and in head and neck squamous carcinoma (Villaret et al, 2000). 14-3-3g expression was increased in response to vessel damage and proliferative signals (Autieri et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel interaction between integrin β1 and 14-3-3β

2001

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“…[7][8][9][10] Previous studies showed that 14-3-3 might be a potential oncogene. 11 Together, these findings enhance our perceptions of 14-3-3 as a potential oncogene.…”

Section: Introductionmentioning

confidence: 99%