Elevated Cytomegalovirus and Epstein-Barr virus burden in rheumatoid arthritis: A true pathogenic role or just a coincidence

Bassyouni, Rasha H.; Dwedar, Reham Ali; Ezzat, Eman; Marzaban, Raghda; Nassr, Maha H.; Rashid, Laila

doi:10.1016/j.ejr.2019.03.003

Cited by 9 publications

(7 citation statements)

References 31 publications

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“…This result was related to another study showing that helps T (Th) cells participate in RA pathogenesis, especially Th17 cells, which produce IL-21, IL-22, and TNFα [16] . Similarly, we found that DCs are regulated through human cytomegalovirus (CMV) infection (Figure 3e), a nding consistent with research showing that RA patients usually have CMV [17] .…”

Section: Differential Chromosome Accessibility Analysis In Rheumatoid...supporting

confidence: 90%

Gene regulatory network study of rheumatoid arthritis in single-cell chromatin landscapes of peripheral blood mononuclear cells

Zhang

Hong

et al. 2022

Modern Rheumatology

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Objectives Assays for transposase-accessible chromatin with single-cell sequencing (scATAC-seq) contribute to the progress in epigenetic studies. The purpose of our project was to discover the transcription factors (TFs) that were involved in the pathogenesis of RA at single-cell resolution using epigenetic technology. Methods Peripheral blood mononuclear cells (PBMCs) of 7 RA patients and 7 natural controls were extracted nuclei suspensions for library construction. Subsequently, scATAC-seq was performed to generate a high-resolution map of active regulatory DNA for bioinformatics analysis. Results We obtained 22 accessible chromatin patterns. Then, 10 key TFs were involved in the RA pathogenesis by regulating the activity of MAP kinase. Consequently, two genes (PTPRC, SPAG9) regulated by 10 key TFs were found that may be associated with RA disease pathogenesis and these TFs were obviously enriched in RA patients (p<0.05, FC>1.2). With further qPCR validation on PTPRC and SPAG9 in monocytes, we found differential expression of these two genes, which were regulated by eight TFs (ZNF384, HNF1B, DMRTA2, MEF2A, NFE2L1, CREB3L4 (var. 2), FOSL2::JUNB (var. 2), MEF2B), showing highly accessible binding sites in RA patients. Conclusions These findings demonstrate the value of using scATAC-seq to reveal transcriptional regulatory variation in RA-derived PBMCs, providing insights on therapy from an epigenetic perspective.

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Section: Differential Chromosome Accessibility Analysis In Rheumatoid...supporting

confidence: 90%

Gene regulatory network study of rheumatoid arthritis in single-cell chromatin landscapes of peripheral blood mononuclear cells

Zhang

Hong

et al. 2022

Modern Rheumatology

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“…Another limitation of the study is that we could not recruit any treatment‐naive patients; being a tertiary referral center, all patients had received some immunosuppression treatment. Although previous studies on SLE 22,26 and RA 27 patients have reported that the frequency of EBV‐infected cells is independent of treatment with immunosuppressive medications, we cannot exclude the possible role of immunosuppression on EBV reactivation in this study.…”

Section: Discussionmentioning

confidence: 84%

Detection of Epstein–Barr virus in systemic sclerosis patients: A molecular and serological based study

et al. 2022

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Systemic sclerosis (SSc) is a rare chronic connective tissue disease of unknown etiology, characterized by microvasculopathy, immunological abnormalities, and fibrosis of the skin and visceral organs. 1,2 SSc is more common in women than men (ratio between 4:1 and 14:1), usually affecting middle-aged adults. 3,4 It has a higher mortality rate than any other rheumatic disease. 5,6 Epstein-Barr virus (EBV), a lymphotropic virus that chronically affects 90% of the human population, has been proposed as a possible environmental trigger of SSc. [7][8][9][10][11][12] Topoisomerase autoantibodies, a specific marker for SSc, are shown to be produced by EBV-transformed B cells from healthy

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“…Quantitation of the EBV and CMV -DNA was conducted using CMV-and EBV specific primers sequences aiming, the conserved 105-bp region of the major immediate-early antigen in the CMV genome and 97 bp region of the conserved Epstein-Barr nuclear antigen 1 (EBNA-1) region in the EBV genome as designated by the Artus® CMV TM PCR Kit and the Artus® EBV TM PCR Kit (QIAGEN,Valencia, CA), respectively 18 .…”

Section: Quantitation Of Cytomegalovirus (Cmv) and Ebestein Barr Viru...mentioning

confidence: 99%

Cytomegalovirus and Epstein–Barr Virus Infection: Two Triggers flaring up Systemic Lupus Erythematosus Patients

hamed

Bassyouni²,

fathi³

et al. 2022

Egyptian Journal of Medical Microbiology

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Background: Systemic lupus erythematosus (SLE) is an autoimmune, multi-system, chronic inflammatory disease The effect of Cytomegalovirus (CMV) and Epstein-Barr virus(EBV) in triggering SLE has been investigated for many years. Objectives: To study the association of viral load of CMV-EBV in the serum of SLE patients' with SLE disease parameters. Methodology: 48SLE patients and 40controls were enrolled. Disease activity was assessed using SLE disease activity index (SLEDAI). Quantitation of CMV and EBV-DNA in serum were detected by real-time polymerase chain reaction. Results: Patients were 91.8% females and 8.2%males. Mean age was 26.6±8.0 years, mean disease duration 4.5±3.1 years, and age at onset 22.1±7.8 years. 41.7% of SLE patients had CMV-DNA, 54.2% patients had EBV-DNA. Neither EBV-DNA nor CMV-DNA were found in the healthy controls. Copy numbers of CMV and EBV found in the serum of SLE patients were 26827±25879copies/μl, and 25309±22852copies/μl, respectively. Regarding SLEDAI; 83.3% showed high disease activity. Renal biopsy revealed that, 66.7% of the patients had lupus nephritis; 50% with CMV-DNA, and 56.25% with EBV-DNA. Regarding the association between CMV and EBV with different disease parameters in SLE patients; we found significant associations with: photosensitivity, Raynaud 's and thrombocytopenia (pvalue<0.05). EBV-DNA were significantly associated with: pyuria, oral ulcers, photosensitivity, vasculitis and involvement of nervous system (r=0.43, p=0.002), (r=0.36, p=0.01), (r=0.42, p=0.003), (r=-0.33, p=0.023), (r=0.32, p=0.029) respectively. Conclusions: A high incidence of CMV and EBV was detected in SLE patients with increased viral load. Disease activity of SLE patients is significantly higher in patients infected with CMV and EBV compared to non-infected.

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Elevated Cytomegalovirus and Epstein-Barr virus burden in rheumatoid arthritis: A true pathogenic role or just a coincidence

Cited by 9 publications

References 31 publications

Gene regulatory network study of rheumatoid arthritis in single-cell chromatin landscapes of peripheral blood mononuclear cells

Gene regulatory network study of rheumatoid arthritis in single-cell chromatin landscapes of peripheral blood mononuclear cells

Detection of Epstein–Barr virus in systemic sclerosis patients: A molecular and serological based study

Cytomegalovirus and Epstein–Barr Virus Infection: Two Triggers flaring up Systemic Lupus Erythematosus Patients

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