Elevated Concentration of Microvesicles Isolated from Peripheral Blood in Breast Cancer Patients

Galindo-Hernández, Octavio; Villegas-Comonfort, Sócrates; Candanedo, Fernando; González-Vázquez, María Cristina; Chávez-Ocaña, Sonia; Jiménez-Villanueva, Xicotencatl; Sierra-Martínez, Mónica; Salazar, Eduardo Pérez

doi:10.1016/j.arcmed.2013.03.002

Cited by 133 publications

(117 citation statements)

References 38 publications

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“…Levels of MVs expressing tumor-associated proteins such as MUC1, EGFR or FAK seem to be elevated in the blood of breast cancer patients 15,16 . Also for Exos, recent studies have shown that blood-derived Exos carrying tumor-specific antigens such as Glypican-1 for pancreatic cancer or Del-1 for breast cancer allow early disease detection with high specificity and sensitivity 17,18 .…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Microvesicles from Peripheral Blood

Menck

Bleckmann

Schulz

et al. 2017

JoVE

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The release of extracellular vesicles (EVs) including small endosomal-derived exosomes (Exos, diameter < 100 nm) and large plasma membrane-derived microvesicles (MVs, diameter > 100 nm) is a fundamental cellular process that occurs in all living cells. These vesicles transport proteins, lipids and nucleic acids specific for their cell of origin and in vitro studies have highlighted their importance as mediators of intercellular communication. EVs have been successfully isolated from various body fluids and especially EVs in blood have been identified as promising biomarkers for cancer or infectious diseases. In order to allow the study of MV subpopulations in blood, we present a protocol for the standardized isolation and characterization of MVs from peripheral blood samples. MVs are pelleted from EDTA-anticoagulated plasma samples by differential centrifugation and typically possess a diameter of 100 -600 nm. Due to their larger size, they can easily be studied by flow cytometry, a technique that is routinely used in clinical diagnostics and available in most laboratories. Several examples for quality control assays of the isolated MVs will be given and markers that can be used for the discrimination of different MV subpopulations in blood will be presented.

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Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Microvesicles from Peripheral Blood

Menck

Bleckmann

Schulz

et al. 2017

JoVE

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“…RAB27A loss of function in 4T1 breast cancer cells inhibited both primary tumor growth and lung metastasis (12). Elevated concentrations of MVs have been reported in the peripheral blood of patients with breast cancer (5).…”

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confidence: 99%

Hypoxia-inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis

Wang

Gilkes²,

Takano³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

402

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Extracellular vesicles such as exosomes and microvesicles (MVs) are shed by cancer cells, are detected in the plasma of cancer patients, and promote cancer progression, but the molecular mechanisms regulating their production are not well understood. Intratumoral hypoxia is common in advanced breast cancers and is associated with an increased risk of metastasis and patient mortality that is mediated in part by the activation of hypoxiainducible factors (HIFs). In this paper, we report that exposure of human breast cancer cells to hypoxia augments MV shedding that is mediated by the HIF-dependent expression of the small GTPase RAB22A, which colocalizes with budding MVs at the cell surface. Incubation of naïve breast cancer cells with MVs shed by hypoxic breast cancer cells promotes focal adhesion formation, invasion, and metastasis. In breast cancer patients, RAB22A mRNA overexpression in the primary tumor is associated with decreased overall and metastasis-free survival and, in an orthotopic mouse model, RAB22A knockdown impairs breast cancer metastasis.orthotopic transplantation | triple negative breast cancer | oxygen | tumor microenvironment | mammary fat pad implantation

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“…EVs can thus be recovered from biological fluids and cell cultures for monitoring of specific pathophysiological processes. Of note, elevated levels of EVs have been found in the circulation of patients with cancer, including those with liver, breast and ovarian cancer [58][59][60][61][62][63]; this may be related to the increased proliferative activity of malignant cells as well as the abnormal, leaky tumour vasculature which allows escape of EVs to the circulation. These findings, together with the notion that the RNA and protein profiles of EVs closely reflect the cell or tissue of origin, have stimulated efforts to establish EVs as biomarkers of prognosis and treatment response monitoring in cancer.…”

Section: Evs For Liquid Biopsy In Cancermentioning

confidence: 99%