Elevated Circulating hsa-miR-106b, hsa-miR-26a, and hsa-miR-29b in Type 2 Diabetes Mellitus with Diarrhea-Predominant Irritable Bowel Syndrome

Wenhua, Tao; Dong, Xiaoyun; Kong, Guimei; Fang, Penghua; Huang, Xiaoli; Bo, Ping

doi:10.1155/2016/9256209

Cited by 22 publications

(16 citation statements)

References 38 publications

(43 reference statements)

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“…Furthermore, elevated urinary miR-29a expression was demonstrated to be significantly correlated with albuminuria and carotid intima-media thickness in patients with T2DM (16). Circulating miR-29b in patients also increases with T2DM and diarrhea-predominant irritable bowel syndrome (17). Taken together, these data indicate the crucial role of miR-29 in IR and T2DM.…”

Section: Microrna-29a Is Involved Lipid Metabolism Dysfunction and Inmentioning

confidence: 74%

MicroRNA‑29a is involved lipid metabolism dysfunction and insulin resistance in C2C12 myotubes by targeting PPARδ

Wang

Jiang

et al. 2018

Mol Med Report

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MicroRNA‑29a (miR‑29a) expression has been reported to be closely associated with skeletal muscle insulin resistance and type 2 diabetes. The present study investigated the effect of miR‑29a on palmitic acid (PA)‑induced lipid metabolism dysfunction and insulin resistance in C2C12 myotubes via overexpressing or silencing of miR‑29a expression. Mouse C2C12 myoblasts were cultured, differentiated and transfected with miR‑29a or miR‑29a inhibitor lentiviral with or without subsequent palmitic acid (PA) treatment. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blot analysis were performed to assess the mRNA and protein levels of related genes, respectively. PA treatment increased the expression of miR‑29a in a time‑ and dose‑ dependent manner. miR‑29a silencing improved insulin‑induced glucose uptake and increased glucose transporter‑4 (GLUT4) transportation to the plasma membrane by upregulating its target peroxisome proliferator‑activated receptor δ (PPARδ). Furthermore, it was observed that miR‑29a regulated the expression of genes associated with lipid metabolism, including pyruvate dehydrogenase kinase isoform, mitochondrial uncoupling protein (UCP)2, UCP3, long chain specific acyl‑CoA dehydrogenase, mitochondrial and fatty acid transport protein 2. The results confirmed that silencing miR‑29a induced a decrease in glucose transport and affected lipid metabolism in PA‑treated C2C12 cells, and therefore may be involved in insulin resistance by targeting PPARδ in skeletal muscle. Therefore, the inhibition of miR‑29a may be a potential novel strategy for treating insulin resistance and type 2 diabetes.

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Section: Microrna-29a Is Involved Lipid Metabolism Dysfunction and Inmentioning

confidence: 74%

MicroRNA‑29a is involved lipid metabolism dysfunction and insulin resistance in C2C12 myotubes by targeting PPARδ

Wang

Jiang

et al. 2018

Mol Med Report

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“…Growing evidence indicates that miRNAs are involved in the pathogenesis of T2DM, resulting in increased glucagon secretion and reduced incretin response (26)(27)(28). miRNAs have been implicated in the epigenetic regulation of key metabolic, inflammatory, and antiangiogenic pathways in T2DM.…”

Section: Discussionmentioning

confidence: 99%

miR‑34a and miR‑125b are upregulated in peripheral blood mononuclear cells from patients with type 2 diabetes mellitus

Yan-xin

Pan

et al. 2017

Exp Ther Med

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Abstract. Type 2 diabetes mellitus (T2DM) is a leading cause of blindness, non-traumatic amputation and end-stage renal disease, as well as a major cardiovascular risk factor. To determine whether miR-125b and miR-34a serve an important role in the development of T2DM, the current study investigated the expression profile of two microRNAs (miR-34a and miR-125b) and their relative genes in peripheral blood mononuclear cells from 73 patients with T2DM and 52 healthy donors by reverse transcription-quantitative polymerase chain reaction In addition, the association between miR-34a, miR-125b and their relevant genes expression profile were analyzed with respect to the pathogenesis of T2DM. The present study demonstrated that the expression levels of miR-125b and miR-34a were elevated in peripheral blood mononuclear cell samples from patients with T2DM. Furthermore, miR-34a and miR-125b were positively correlated with low-density lipoprotein/high-density lipoprotein (HDL) and Foxp3 and negatively related to triglyceride/HDL. However, no correlation among miR-34a, miR-125b and the value of homeostasis model assessment of insulin resistance, homeostasis model assessment of β-cell function and the genes of B lymphocyte-induced maturation protein-1, interferon regulatory factor-4, P53 and retinoid-related orphan receptor γt were observed. These results indicate that the alteration of miR-34a and miR-125b exists in patients with T2DM, which may be involved in the pathogenesis of T2DM, and could be a potential novel biomarker of T2DM. IntroductionType 2 diabetes mellitus (T2DM), formerly termed non-insulin-dependent diabetes mellitus or adult-onset diabetes, is a multifactor disease that involves complex interactions between genes (1-3), abnormalities of the immune system (4-6), environmental factors (7-10) and health-impacting behavior (11,12), and represents a serious public health problem in numerous developed countries (13). Current investigations have revealed a definite global increase in the incidence and prevalence of diabetes. In 2013, 382 million people worldwide were estimated to be diabetic by the International Diabetes Federation, which is expected to rise to 592 million cases in the year 2035 (14). As a result of the increasing rate of diabetes and its widespread societal and economic consequences, prevention of diabetes among people at high risk is an important public health issue in clinic practice. However, the extent to which multiple defects in the regulation of lipids, insulin secretion and action, and the immune system contribute to the pathogenesis of T2DM has yet to be elucidated.MicroRNAs (miRNAs) are a class of small, single-stranded non-coding RNAs (~22 nucleotides) that are transcribed from the DNA of a gene, and modulate the expression of a network of mRNAs through binding to the 3'-untranslated region (3'-UTR), 5'-UTR or to the open reading frame of target mRNAs (15). Notably, each miRNA is able to target multiple mRNAs. Growing evidence indicates that miRNAs are involved in T2DM (16). However,...

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“…The involvement of the mir-218-5p, mir-98-5p, and mir-3135b with T2D and bone disease, however, has not been reported in the literature. However, overexpression of mir-29b-3p in T2D and in diarrhea-predominant irritable bowel syndrome is seen [68], and it has been shown that diabetes affects tissue expression of mir-29c-3p [69].…”

Section: Discussionmentioning

confidence: 99%

Network-Based Computational Approach to Identify Delineating Common Cell Pathways Influencing Type 2 Diabetes and Diseases of Bone and Joints

Moni

Islam

Rahman³

et al. 2020

IEEE Access

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Developing type 2 diabetes (T2D) can increase patient risk of developing other common diseases and exacerbate their severity, including diseases that affect bone and joints. Such comorbidity interactions are hard to study in detail by traditional endocrinological methods. Thus, we developed tissue transcript analytical approaches to identify common pathways through which these diseases can interact. We examined RNAseq and microarray transcript datasets from studies of T2D and chronic bone and joint diseases, namely rheumatoid arthritis (RA), osteoarthritis (OA), juvenile idiopathic arthritis (JIA) and low peak bone density, a key osteoporosis (OP) determinant. These datasets contained data from affected individuals and matched controls. Differentially expressed genes (DEGs) for each condition were compared with T2D DEG. Overlapping DEGs (i.e., those common to T2D and a bone or joint condition) were subjected to gene enrichment by pathway analyses and by gene ontology methods, and the results were evaluated by using SNP-disease linkage (dbGaP) and gene-disease association (OMIM) databases that indicate gene involvement in pathologies. By examining gene targets of transcription factors (TFs) and microRNA (miRNAs), we also constructed DEG-TF and DEG-miRNA interactions networks for analysis. We identified strong candidate genes in common pathways, notably including SYK, UCP3, ROR1, PPARG, BUB1, AKT2, ADCY2 and CCR5. The DEG-TF network and DEG-miRNA interactions network analyses revealed a number of TFs (GATA2, FOXC1, USF2, YY1, E2F1, JUN, RELA, CREB1, TFAP2A, NFB1) and miRNAs (mir-335-5p, mir-16-5p, mir-26b-5p, mir-124-3p, mir-218-5p, mir-98-5p, mir-29b-3p, mir-3135b, mir-29c-3p, mir-1-1) that can regulate the identified DEGs at the transcriptional and post-transcriptional levels. Thus this data-driven approach has enabled identification and validation of regulatory factors and cell pathways by which T2D may influence bone and joint conditions, which may suggest new ways to interfere with the pathogenic processes involved.INDEX TERMS T2 diabetes, network-based approach, joint diseases, bone diseases.

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Elevated Circulating hsa-miR-106b, hsa-miR-26a, and hsa-miR-29b in Type 2 Diabetes Mellitus with Diarrhea-Predominant Irritable Bowel Syndrome

Cited by 22 publications

References 38 publications

MicroRNA‑29a is involved lipid metabolism dysfunction and insulin resistance in C2C12 myotubes by targeting PPARδ

MicroRNA‑29a is involved lipid metabolism dysfunction and insulin resistance in C2C12 myotubes by targeting PPARδ

miR‑34a and miR‑125b are upregulated in peripheral blood mononuclear cells from patients with type 2 diabetes mellitus

Network-Based Computational Approach to Identify Delineating Common Cell Pathways Influencing Type 2 Diabetes and Diseases of Bone and Joints

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