Elevated chemokine levels during adult but not pediatric Crimean–Congo hemorrhagic fever

Araslı, Mehmet; Özsürekçi, Yasemin; Elaldı, Nazif; McAuley, Alexander J.; Öncel, Eda Karadağ; Tekin, İshak Özel; Gözel, Mustafa Gökhan; Kaya, Ali; İçağasıoğlu, Füsun Dilara; Çağlayık, Dilek Yağcı; Korukluoğlu, Gülay; Köktürk, Fürüzan; Bakır, Mehmet; Bente, Dennis A.; Ceyhan, Mehmet

doi:10.1016/j.jcv.2015.03.010

Cited by 12 publications

(15 citation statements)

References 29 publications

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“…Platelets maintain the integrity of the vascular system, and have been shown to be critical for the pathogenesis of viral hemorrhagic fevers [33]. CXCL8, ICAM1 and EDN1, all of which were induced in our experiments, are associated with vascular leakage and with worse outcomes in patients [40,47,50]. Excessive release of cytokines and soluble markers likely dysregulate platelet activation.…”

Section: Discussionmentioning

confidence: 61%

Dual RNA-Seq characterization of host and pathogen gene expression in liver cells infected with Crimean-Congo Hemorrhagic Fever Virus

et al. 2020

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Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne virus that can cause a hemorrhagic fever in humans, with a case fatality rate of up to 40%. Cases of CCHFV have been reported in Africa, Asia, and southern Europe; and recently, due to the expanding range of its vector, autochthonous cases have been reported in Spain. Although it was discovered over 70 years ago, our understanding of the pathogenesis of this virus remains limited. We used RNA-Seq in two human liver cell lines (HepG2 and Huh7) infected with CCHFV (strain IbAr10200), to examine kinetic changes in host expression and viral replication simultaneously at 1 and 3 days post infection. Through this, numerous host pathways were identified that were modulated by the virus including: antiviral response and endothelial cell leakage. Notably, the genes encoding DDX60, a cytosolic component of the RIG-I signalling pathway and OAS2 were both shown to be dysregulated. Interestingly, PTPRR was induced in Huh7 cells but not HepG2 cells. This has been associated with the TLR9 signalling cascade, and polymorphisms in TLR9 have been associated with poor outcomes in patients. Additionally, we performed whole-genome sequencing on CCHFV to assess viral diversity over time, and its relationship to the host response. As a result, we have demonstrated that through next-generation mRNA deep-sequencing it is possible to not only examine mRNA gene expression, but also to examine viral quasispecies and typing of the infecting strain.

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Section: Discussionmentioning

confidence: 61%

Dual RNA-Seq characterization of host and pathogen gene expression in liver cells infected with Crimean-Congo Hemorrhagic Fever Virus

et al. 2020

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“…There were no cases lost in a study by Kızılgün et al including 41 CCHF patients between the ages of 1 and 17 (29). Serum chemokines levels of pediatric patients that received CCHF diagnosis have been found lower than those of the adults in a multicenter study (30). Factors like hasty transfer of the pediatric age group to a health center and their lower chemokines response may explain low mortality in the pediatric age group.…”

Section: Discussionmentioning

confidence: 92%

“…The basis of treatment in this disease consists supportive care. When necessary, the patients should be given thrombocyte suspension, freshly frozen plasma and erythrocyte suspension; respiratory, dialysis, and parenteral nutrition support should be provided; and fluid-electrolyte balance should be monitored (30). In our study, two of the 6 patients receiving CCHF diagnosis were given ribavirin and the remaing 4 were followed with supportive care.…”

Section: Discussionmentioning

confidence: 96%

The Evaluation of Clinical and Laboratory Findings of Pediatric Patients Applying with Tick Exposure

Kömürlüoğlu¹,

Arıkan²,

Öncel³

et al. 2017

J Pediatr Inf

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“…Kızılgün ve arkadaşlarının 1-17 yaş arasındaki KKKA tanısı alan 41 çocuk hastada yaptıkları çalışmada da kaybedilen olgu olmamıştır (29). Çok merkezli bir çalışmada KKKA tanılı çocuk hastalarda serum kemokin düzeyi erişkinlere göre daha düşük bulunmuştur (30). Pediatrik yaş grubunun sağlık kuruluşuna daha hızlı götürülmesi, daha düşük kemokin yanıtı gibi faktörler pediatrik yaş grubundaki düşük mortaliteyi açıklayabilmektedir.…”

Section: Gereç Ve Yöntemlerunclassified

“…Gerektiğinde hastalara trombosit süspansiyonu, taze donmuş plazma ve eritrosit süspansiyonu verilmeli; solunum, dolaşım, diyaliz ve parenteral beslenme desteği sağlanmalı; sıvı-elektrolit dengesi takip edilmelidir(30). Çalışmamızda KKKA tanısıyla takip edilen altı hastanın ikisine ribavirin verilmiş, geri kalan dördü ise destek tedavileri ile izlenmiştir.…”

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Kene Tutunması ile Başvuran Çocuk Hastaların Klinik ve Laboratuvar Bulgularının Değerlendirilmesi

Kömürlüoğlu¹,

Arıkan²,

Öncel³

et al. 2017

J Pediatr Inf

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Objective: Crimean-Congo hemorrhagic fever (CCHF) is the most widespread, tick-borne viral disease affecting humans. CCHF first emerged in Turkey in 2002, and the prevalence of the disease has been found to be increased. Central Anatolia Region is endemic for the disease where sporadic cases or even outbreaks are being observed every year. The study was aimed to evaluate sociodemographic, clinical and laboratory parameters of children admitted due to tick bite and prediagnosis of CCHF and to discuss necessity of repeated blood sampling in asymptomatic cases. Material and Methods: Between January 2014 and December 2016, hospitalized or outpatient pediatric cases (< 18 years) whom had tick bite in the Infectious Diseases Unit of Hacettepe University İhsan Doğramacı Children's Hospital were enrolled in the present study. Gender, age, contact region with tick, physical examination and laboratory findings of cases and occurence of CCHF were evaluated. Results: In this study, 74 female (45.4%), 89 male (54.6%), a total of 163 cases were evaluated. The mean age of our cases was 7.49 ± 3.9 years. The most common admission was seen in the month of August. Tick was frequently removed from the region of the head and neck (56.1%). Among patients 6 of whom diagnosed CCHF and discharged without any complication. In patients without CCHF (90.4%) had any symptoms during follow-up and most of them (92.3%) had normal laboratory findings on admission, 3 and 7-10 days. Symptoms such as fever, malaise, vomiting, abdominal pain, headache and rash was significantly common in patients with CCHF (all of them p< 0.001) and they had at least one abnormal laboratory parameter on admission. Özet Giriş: Kırım Kongo kanamalı ateşi (KKKA), insanlarda görülen en sık kene ilişkili hastalıktır. KKKA Türkiye'de ilk olarak 2002 yılında görülmüş ve prevalansı giderek artmıştır. Orta Anadolu hastalık için endemiktir ve her yıl sporadik olgular ya da salgınlar görülebilmektedir. Bu çalışmada kene tutunması şikayetiyle hastanemize başvuran çocuk hastaların demografik özelliklerinin belirlenmesi, klinik bulgularının incelenmesi ve laboratuvar sonuçlarının değerlendirilmesi, asemptomatik olgularda erken dönemde laboratuvar tetkiklerinin gerekliliğinin tartışılması amaçlanmıştır. Gereç ve Yöntemler: Ocak 2014-Aralık 2016 tarihleri arasında Hacettepe Üniversitesi İhsan Doğramacı Çocuk Hastanesi'ne kene ısırığı şikayetiyle ayaktan başvuran çocuk hastalar (< 18 yaş) bu çalışmaya dahil edildi. Cinsiyet, yaş, kenenin tutunduğu bölge, fizik muayene ve laboratuvar bulguları ve KKKA meydana gelme durumu değerlendirildi. Bulgular: Bu çalışmada 74 (%45.4)'ü kız, 89 (%54.6)'u erkek olmak üzere toplam 163 olgu değerlendirildi. Olguların ortalama yaşı 7.49 ± 3.9 yıldı. En sık başvuru Ağustos ayında görüldü (%19). Kene sıklıkla baş-boyun bölgesinden çıkarılmıştı (%56.1). Bu hastalardan altısı KKKA tanısı aldı ve hepsi komplikasyonsuz olarak taburcu edildi. KKKA gelişmeyen hastaların %90.4'ünde takip boyunca herhangi bir semptom görülmedi ve bunların büyük kısmının (%92....

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Elevated chemokine levels during adult but not pediatric Crimean–Congo hemorrhagic fever

Cited by 12 publications

References 29 publications

Dual RNA-Seq characterization of host and pathogen gene expression in liver cells infected with Crimean-Congo Hemorrhagic Fever Virus

Dual RNA-Seq characterization of host and pathogen gene expression in liver cells infected with Crimean-Congo Hemorrhagic Fever Virus

The Evaluation of Clinical and Laboratory Findings of Pediatric Patients Applying with Tick Exposure

Kene Tutunması ile Başvuran Çocuk Hastaların Klinik ve Laboratuvar Bulgularının Değerlendirilmesi

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