Elevated atmospheric CO<sub>2</sub> concentration triggers redistribution of nitrogen to promote tillering in rice

Zhou, Juan; Gao, Yujiao; Wang, Junpeng; Liu, Chang; Zi, Wang; Lv, Minjia; Zhang, Xiaoxiang; Zhou, Yong; Dong, Guichun; Wang, Yulong; Huang, Jianye; Hui, Dafeng; Yang, Zefeng; Yao, Youli

doi:10.1002/pei3.10046

Cited by 5 publications

(2 citation statements)

References 53 publications

(86 reference statements)

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“…In our quest for more universally applicable candidate reference genes, we screened our existing RNA-seq datasets, which comprise responses of two japonica rice varieties to salt stress, one japonica and one indica rice variety to three nitrogen application rate [32], one japonica and one indica rice variety to γ-irradiation dosage [33], and a japonica rice variety to carbon dioxide concentration and nitrogen rate [34] treatments. These datasets include samples from root, shoot, 7-day-young whole plants, filling grain at seven days post anthesis, and shoot tip growth points of rice, containing RNA-seq results from a total of 72 biological samples of various tissues.…”

Section: Screening For Stably Expressed Reference Genes From Rna-seq ...mentioning

confidence: 99%

Validation of Novel Reference Genes in Different Rice Plant Tissues through Mining RNA-Seq Datasets

Liu,

Gao,

Zhao

et al. 2023

Plants

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Reverse transcription quantitative real-time PCR (RT-qPCR) is arguably the most prevalent and accurate quantitative gene expression analysis. However, selection of reliable reference genes for RT-qPCR in rice (Oryza sativa) is still limited, especially for a specific tissue type or growth condition. In this study, we took the advantage of our RNA-seq datasets encompassing data from five rice varieties with diverse treatment conditions, identified 12 novel candidate reference genes, and conducted rigorous evaluations of their suitability across typical rice tissues. Comprehensive analysis of the leaves, shoots, and roots of two rice seedlings subjected to salt (30 mmol/L NaCl) and drought (air-dry) stresses have revealed that OsMED7, OsACT1, and OsOS-9 were the robust reference genes for leaf samples, while OsACT1, OsZOS3-23, and OsGDCP were recommended for shoots and OsMED7, OsOS-9, and OsGDCP were the most reliable reference genes for roots. Comparison results produced by different sets of reference genes revealed that all these newly recommended reference genes displayed less variation than previous commonly used references genes under the experiment conditions. Thus, selecting appropriate reference genes from RNA-seq datasets leads to identification of reference genes suitable for respective rice tissues under drought and salt stress. The findings offer valuable insights for refining the screening of candidate reference genes under diverse conditions through the RNA-seq database. This refinement serves to improve the accuracy of gene expression in rice under similar conditions.

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Section: Screening For Stably Expressed Reference Genes From Rna-seq ...mentioning

confidence: 99%

Validation of Novel Reference Genes in Different Rice Plant Tissues through Mining RNA-Seq Datasets

Liu,

Gao,

Zhao

et al. 2023

Plants

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“…However, in addition to genetic factors, it is clear that environmental factors can also influence tiller development (Wang and Li 2005;Assuero and Tognetti 2010;Saad 2014). These include the circadian clock (Wang et al 2020), temperature (Xu et al 2020), CO 2 (Seneweera 2011;Zhou et al 2021;Liu et al 2021) and the availability of nutrients (Wang et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Elevated CO2 Priming as a Sustainable Approach to Increasing Rice Tiller Number and Yield Potential

Sloan

Mujab

Mashitah

et al. 2023

Rice

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Tillering and yield are linked in rice, with significant efforts being invested to understand the genetic basis of this phenomenon. However, in addition to genetic factors, tillering is also influenced by the environment. Exploiting experiments in which seedlings were first grown in elevated CO2 (eCO2) before transfer and further growth under ambient CO2 (aCO2) levels, we found that even moderate exposure times to eCO2 were sufficient to induce tillering in seedlings, which was maintained in plants grown to maturity plants in controlled environment chambers. We then explored whether brief exposure to eCO2 (eCO2 priming) could be implemented to regulate tiller number and yield in the field. We designed a cost-effective growth system, using yeast to increase the CO2 level for the first 24 days of growth, and grew these seedlings to maturity in semi-field conditions in Malaysia. The increased growth caused by eCO2 priming translated into larger mature plants with increased tillering, panicle number, and improved grain filling and 1000 grain weight. In order to make the process more appealing to conventional rice farmers, we then developed a system in which fungal mycelium was used to generate the eCO2 via respiration of sugars derived by growing the fungus on lignocellulosic waste. Not only does this provide a sustainable source of CO2, it also has the added financial benefit to farmers of generating economically valuable oyster mushrooms as an end-product of mycelium growth. Our experiments show that the system is capable of generating sufficient CO2 to induce increased tillering in rice seedlings, leading eventually to 18% more tillers and panicles in mature paddy-grown crop. We discuss the potential of eCO2 priming as a rapidly implementable, broadly applicable and sustainable system to increase tillering, and thus yield potential in rice.

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Mutation at Grassy tiller 1 increases rice yield production and resistance to sheath blight

Yang,

Kumar,

Jia

et al. 2024

Crop Health

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Elevated atmospheric CO₂ concentration triggers redistribution of nitrogen to promote tillering in rice

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 5 publications

References 53 publications

Validation of Novel Reference Genes in Different Rice Plant Tissues through Mining RNA-Seq Datasets

Validation of Novel Reference Genes in Different Rice Plant Tissues through Mining RNA-Seq Datasets

Elevated CO2 Priming as a Sustainable Approach to Increasing Rice Tiller Number and Yield Potential

Mutation at Grassy tiller 1 increases rice yield production and resistance to sheath blight

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Elevated atmospheric CO2 concentration triggers redistribution of nitrogen to promote tillering in rice

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 5 publications

References 53 publications

Validation of Novel Reference Genes in Different Rice Plant Tissues through Mining RNA-Seq Datasets

Validation of Novel Reference Genes in Different Rice Plant Tissues through Mining RNA-Seq Datasets

Elevated CO2 Priming as a Sustainable Approach to Increasing Rice Tiller Number and Yield Potential

Mutation at Grassy tiller 1 increases rice yield production and resistance to sheath blight

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Product

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Elevated atmospheric CO₂ concentration triggers redistribution of nitrogen to promote tillering in rice