Elevated ammonium reduces the negative effect of heat stress on the stony coral Pocillopora damicornis

Zhou, Zhi; Zhang, Guoqing; Chen, Guangmei; Ni, Xingzhen; Guo, Liping; Yu, Xiaopeng; Xiao, Chunlin; Xu, Yanlai; Shi, Xiaowei; Huang, Bo

doi:10.1016/j.marpolbul.2017.03.018

Cited by 33 publications

(17 citation statements)

References 43 publications

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“…Recently, Zhou et al. () demonstrated that five gene ontology (GO) terms overrepresented in heat stressed P. damicornis were related to TNF signaling pathway, apoptosis and cell death (Zhou et al., ). Similar results were also reported in Acropora aspera (Rosic et al., ), A. hyacinthus (Seneca & Palumbi, ) and Orbicella faveolata (Pinzón et al., ).…”

Section: Resultsmentioning

confidence: 99%

Dynamics of coral‐associated microbiomes during a thermal bleaching event

et al. 2018

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Coral‐associated microorganisms play an important role in their host fitness and survival. A number of studies have demonstrated connections between thermal tolerance in corals and the type/relative abundance of Symbiodinium they harbor. More recently, the shifts in coral‐associated bacterial profiles were also shown to be linked to the patterns of coral heat tolerance. Here, we investigated the dynamics of Porites lutea‐associated bacterial and algal communities throughout a natural bleaching event, using full‐length 16S rRNA and internal transcribed spacer sequences (ITS) obtained from PacBio circular consensus sequencing. We provided evidence of significant changes in the structure and diversity of coral‐associated microbiomes during thermal stress. The balance of the symbiosis shifted from a predominant association between corals and Gammaproteobacteria to a predominance of Alphaproteobacteria and to a lesser extent Betaproteobacteria following the bleaching event. On the contrary, the composition and diversity of Symbiodinium communities remained unaltered throughout the bleaching event. It appears that the switching and/or shuffling of Symbiodinium types may not be the primary mechanism used by P. lutea to cope with increasing seawater temperature. The shifts in the structure and diversity of associated bacterial communities may contribute more to the survival of the coral holobiont under heat stress.

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Section: Resultsmentioning

confidence: 99%

Dynamics of coral‐associated microbiomes during a thermal bleaching event

et al. 2018

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“…NH 4 Cl was added to each N-treatment aquaria to increase the ammonium concentration by 20 µM, with water changes and aquarium cleaning every 2-3 days (including control aquariums). Ammonium concentrations were selected based on existing literature and the goals of our study (Muscatine et al, 1998;Zhou et al, 2017).…”

Section: Ammonium Enrichmentmentioning

confidence: 99%

Increased Algal Symbiont Density Reduces Host Immunity in a Threatened Caribbean Coral Species, Orbicella faveolata

et al. 2020

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Scleractinian corals are the principal builders of coral reefs. These megadiverse ecosystems are declining due to coral mortality from a variety of stressors, including disease. Corals are dependent upon symbiotic dinoflagellates in the family Symbiodiniaceae for phototrophic contributions to their energy budgets. However, suppression of host immunity may be necessary to maintain these intracellular symbioses. To explore the consequences of symbiosis on host immunity, we manipulated symbiont density by increasing nitrogen availability. Replicate cores from four colonies of the Caribbean coral, Orbicella faveolata, were reared in seawater treated with ammonium for 1 month to increase symbiont density. Corals were then immunestimulated using lipopolysaccharide and poly I:C. Gene expression was analyzed using RNAseq and symbiont density was quantified (as symbiont:host cell ratio) using quantitative PCR (qPCR). Ammonium treatment had limited positive effects on host immunity. In contrast, increases in symbiont density had large negative effects on host expression of immune-related transcripts. These results suggest links between nutrient enrichment and coral disease may be the result of the effect of increased symbiont density on host immunity, rather than the direct effect of the nutrients. Further study of the trade-offs between symbiont density and immunity may help understand how decreasing water quality and increasing disease will shape future reef communities.

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“…The transcriptomes of stony coral P. damicornis were sequenced under high temperature and elevated ammonium, and the differentially expressed genes responsive to heat and ammonium stresses were also obtained after read mapping and abundance estimation (Zhou et al 2017). The expression levels of these coral genes whose coded proteins contained the HSP70 domain were retrieved in the Control (without any treatment), Heat (under heat stress), and Heat_NH4 (under the combined stress of heat and ammonium) groups, and meanwhile, the significant difference of their expression changes was analyzed further.…”

Section: Transcriptome Analysismentioning

confidence: 99%

“…Secondly, PdHSP70 might inhibit directly the apoptosis under heat stress. Because apoptosis has been observed as a main cellular process during coral bleaching (Zhou et al 2017;Kvitt et al 2015;Tchernov et al 2011), PdHSP70 could play a crucial role in preventing the bleaching development of the stony coral P. damicornis. It suggested that PdHSP70 could sustain its refolding activity under high temperature, and it might reduce the bleaching induction of the stony coral P. damicornis under high temperature.…”

Section: Controlmentioning

confidence: 99%

Transcriptome, expression, and activity analyses reveal a vital heat shock protein 70 in the stress response of stony coral Pocillopora damicornis

Zhang

Zhou

Wang

et al. 2018

Cell Stress and Chaperones

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Coral bleaching occurs worldwide with increasing frequencies and intensities, which is caused by the stress response of stony coral to environmental change, especially increased sea surface temperature. In the present study, transcriptome, expression, and activity analyses were employed to illustrate the underlying molecular mechanisms of heat shock protein 70 (HSP70) in the stress response of coral to environmental changes. The domain analyses of assembled transcripts revealed 30 HSP70 gene contigs in stony coral Pocillopora damicornis. One crucial HSP70 (PdHSP70) was observed, whose expressions were induced by both elevated temperature and ammonium after expression difference analysis. The complete complementary DNA (cDNA) sequence of PdHSP70 was identified, which encoded a polypeptide of 650 amino acids with a molecular weight of 71.93 kDa. The deduced amino acid sequence of PdHSP70 contained a HSP70 domain (from Pro8 to Gly616), and it shared the highest similarity (95%) with HSP70 from Stylophora pistillata. The expression level of PdHSP70 gene increased significantly at 12 h, and returned to the initial level at 24 h after the stress of high temperature (32 °C). The cDNA fragment encoding the mature peptide of PdHSP70 was recombined and expressed in the prokaryotic expression system. The ATPase activity of recombinant PdHSP70 protein was determined, and it did not change significantly in a wide range of temperature from 25 to 40 °C. These results collectively suggested that PdHSP70 was a vital heat shock protein 70 in the stony coral P. damicornis, whose mRNA expression could be induced by diverse environmental stress and whose activity could remain stable under heat stress. PdHSP70 might be involved in the regulation of the bleaching owing to heat stress in the stony coral P. damicornis.

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Elevated ammonium reduces the negative effect of heat stress on the stony coral Pocillopora damicornis

Cited by 33 publications

References 43 publications

Dynamics of coral‐associated microbiomes during a thermal bleaching event

Dynamics of coral‐associated microbiomes during a thermal bleaching event

Increased Algal Symbiont Density Reduces Host Immunity in a Threatened Caribbean Coral Species, Orbicella faveolata

Transcriptome, expression, and activity analyses reveal a vital heat shock protein 70 in the stress response of stony coral Pocillopora damicornis

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