Eleutheroside E decreases oxidative stress and NF-κB activation and reprograms the metabolic response against hypoxia-reoxygenation injury in H9c2 cells

Wang, Shanyue; Yang, Xinwei

doi:10.1016/j.intimp.2020.106513

Cited by 31 publications

(16 citation statements)

References 45 publications

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“…NF‐κB was a recognized transcription factor in modulation of inflammation, oxidative stress and cell apoptosis in the context of MIRI 17‐19 . The expression level of phospho‐NF‐κB p65 was significantly upregulated after H/R compared with the control cells.…”

Section: Resultsmentioning

confidence: 99%

“…NF-κB was a recognized transcription factor in modulation of inflammation, oxidative stress and cell apoptosis in the context of MIRI. [17][18][19] The expression level of phospho-NF-κB p65 was significantly upregulated after H/R compared with the control cells. While H9c2 cells were transfected with Ad-shRNA-PCAF, the protein level of The results of Co-IP showed that there was a direct interaction between PCAF and NF-κB p65, and after H/R induction, the interaction between PCAF and NF-κB became stronger (Figure 6C).…”

Section: The Protective Effect Of Pcaf Downregulation In Miri Depends On the Inactivation Of The Nf-κb Pathwaymentioning

confidence: 98%

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Downregulation of p300/CBP‐associated factor inhibits cardiomyocyte apoptosis via suppression of NF‐κB pathway in ischaemia/reperfusion injury rats

Qiu

Liu

et al. 2021

J Cellular Molecular Medi

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Section: Resultsmentioning

confidence: 99%

Section: The Protective Effect Of Pcaf Downregulation In Miri Depends On the Inactivation Of The Nf-κb Pathwaymentioning

confidence: 98%

Downregulation of p300/CBP‐associated factor inhibits cardiomyocyte apoptosis via suppression of NF‐κB pathway in ischaemia/reperfusion injury rats

Qiu

Liu

et al. 2021

J Cellular Molecular Medi

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“…23 It has been validated that the NF-κB subunit p65 is activated during MI/R. 24,25 Activated NF-κB subunit p65 is activated by phosphorylation, transfers from the cytoplasm into the nucleus. It then mediates the expression of genes related to a wide variety of biological functions, including inflammatory cytokines, such as, TNF-α, IL-1β, and IL-6.…”

Section: Discussionmentioning

confidence: 99%

Amelioratory effect of neoandrographolide on myocardial ischemic‐reperfusion injury by its anti‐inflammatory and anti‐apoptotic activities

Liu

Zhang

et al. 2021

Environmental Toxicology

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In the present study, we aimed to evaluate the cardioprotective effect of neoandrographolide (Neo) on myocardial ischemia/reperfusion injury (I/R) models and explore its possible mechanism. We randomly and equally divided male mice into sham-operation, I/R, and I/R + Neo groups. H9C2 cell line and primary neonatal rat cardiomyocytes were induced into the simulated I/R's status and used to further validate the Neo's role in vitro. Heart systolic function, indexes of myocardial injury (IMI), infarct size, pathological change, cell apoptosis, inflammatory cytokines, and indexes related to apoptotic and NF-κB signaling pathways were analyzed in vivo or in vitro after the Neo treatment. Compared to the I/R group, Neo significantly suppressed IMI, infarct size, inflammatory cell infiltration, cell apoptosis, inflammatory cytokines, bax, cleaved caspase-3, P-IKBa, and P-NF-κB protein expressions, and the translocation of NF-kB subunit p65 from the cytoplasm to the nucleus in vivo or in vitro. Still, ejected fraction, fractional shortening, and the bcl-2 protein expression were notably increased after the Neo treatment. Neo could be developed into a new drug for treating myocardial I/R by inhibiting myocardial inflammation and apoptosis, which was closely related to suppressing the activation of bax/bcl-2 and NF-κB signaling pathways.

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“…Sucrose is an energy carrier, which provides plants with the energy and carbon needed for growth and development and also improves their antioxidant activity [32,33]. Previous studies report that eleutherosides E and B have significant antioxidant and neuroprotective effects and can delay the progression of AD [34,35]. Acanthoside B can improve the symptoms caused by scopolamine in amnestic mice by regulating cholinergic function and restoring antioxidant status [36].…”

Section: Biological Significance Of Related Metabolitesmentioning

confidence: 99%

Different Metabolites in the Roots, Seeds, and Leaves of Acanthopanax senticosus and Their Role in Alleviating Oxidative Stress

Wang²,

et al. 2021

Journal of Analytical Methods in Chemistry

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In this study, we examined the metabolites from different parts of Acanthopanax senticosus and their role in alleviating damage caused by oxidative stress. We used UHPLC-QTOF-MS to analyze the chemical components in the root, seed, and leaf extracts of A. senticosus. Two multivariate statistical analysis methods—namely, principal component analysis and partial least square discriminant analysis—were used to distinguish the samples obtained from different parts of the plant. Using univariate statistics, 130 different metabolites were screened out. Among these, the relative content of flavonoids and terpenoids was found to be highest in the leaves, the lignin and phenolic acid content was highest in the roots, and the amino acid and phenolic acid levels were highest in seeds. An MTT assay was used to test the anti-H2O2 oxidative damage to PC12 cells in different parts of the sample. Lastly, using Pearson’s correlation analysis, various metabolites from different parts of A. senticosus were correlated with their antioxidant effects from the corresponding parts. Fifty-two related different metabolites were found, of which 20 metabolites that were positively correlated to oxidative stress were present at a relatively higher level in the roots, whereas 32 metabolites that were negatively correlated were present at relatively higher levels in the seeds and leaves. The results of this study reveal the distribution characteristics and the antioxidant activity of different metabolites of A. senticosus and provide a reference for the rational development of its medicinal parts.

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Eleutheroside E decreases oxidative stress and NF-κB activation and reprograms the metabolic response against hypoxia-reoxygenation injury in H9c2 cells

Cited by 31 publications

References 45 publications

Downregulation of p300/CBP‐associated factor inhibits cardiomyocyte apoptosis via suppression of NF‐κB pathway in ischaemia/reperfusion injury rats

Downregulation of p300/CBP‐associated factor inhibits cardiomyocyte apoptosis via suppression of NF‐κB pathway in ischaemia/reperfusion injury rats

Amelioratory effect of neoandrographolide on myocardial ischemic‐reperfusion injury by its anti‐inflammatory and anti‐apoptotic activities

Different Metabolites in the Roots, Seeds, and Leaves of Acanthopanax senticosus and Their Role in Alleviating Oxidative Stress

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