Elementary processes of the magnesium ion-dependent adenosine triphosphatase activity of heavy meromysin. A transient kinetic approach to the study of kinases and adenosine triphosphatases and a colorimetric inorganic phosphate assay in situ

Abstract

Transient kinetic studies of Mg2+-dependent heavy-meromyosin ATPase (adenosine triphosphatase) were done by monitoring the release of both ADP and Pi into the reaction medium by using linked assay systems. The release of Pi was monitored by its quantitative transfer to ADP, with concomitant reduction of NAD+ in the presence of D-glyceraldehyde 3-phosphate, D-glyceraldehyde 3-phosphate dehydrogenase and phosphoglycerate kinase. The dissociation rates of the products, ADP and Pi, from heavy meromyosin were shown to be faster than the rate-controlling process, which occurs after the initial bond cleavage of ATP. The chromophoric ATP analogue, 6-mercapto-9-f-D-ribofuranosylpurine 5'-triphosphate (thioATP) was used as a substrate and spectral changes associated with a single turnover of heavy meromyosin could be assigned to elementary processes ofthe mechanism. It was shown that the dissociation rate ofthioADP was not the rate-controlling process of the thioATPase, whose catalytic-centre activity was 7.6 times that of the ATPase at pH8. The dissociation rate of ADP from heavy meromyosin was measured by using thioATP as displacing agent and was found to be 2.3 s-1, which is about 50 times the catalytic-centre activity of the ATPase at pH8. Transient kinetic studies with chromophoric adenosine phosphate analogues have general application for kinases and ATPases both in characterizing the chemical states of the intermediates and in delineating the elementary processes of the enzyme mechanism.

A Specific 1:1 G‐actin: DNAase I complex formed by the action of DNAase I on F‐actin

“…ATPase activity was measured using an Aminco spectrophotometer using the optical method as described by Trentham et al [8]. Fluorescence of eATP-G-actin (1,N6-ethenoadenosine triphosphate) was measured at 410 nm in a Perkin-Elmer MPF-3 Fluorospectrophotometer exciting at 340 nm.…”

Myosin-10 produces its power-stroke in two phases and moves processively along a single actin filament under low load

“…Steady-state ATPase measurements were measured using an NADH-coupled assay as previously described (72)(73)(74). Stopped flow experiments were performed using a SF-2001 stopped-flow apparatus (KinTek Corp.) at 25°C.…”

The Binding Constant of ATP to Myosin S1 Fragment

“…The application of rapid kinetic techniques to the study of myosin ATPase established the basic characteristic features of the ATP hydrolysis, namely the relatively rapid cleavage of ATP on the enzyme, followed by the very slow release of products [1,2]. It was seen that the rate constant for ATP dissociation was much slower than for the cleavage step [3] and in fact much slower than the steady-state rate constant [4].…”